口腔医学研究

口腔医学研究 ›› 2018, Vol. 34 ›› Issue (7): 774-779.DOI: 10.13701/j.cnki.kqyxyj.2018.07.022

• 涎腺疾病研究 • 上一篇    下一篇

水通道蛋白5和细胞程序性死亡分子5在大鼠腮腺主导管结扎再通后表达的实验研究

唐学敏1, 孙长华2, 朱玉红3, 王晶4, 杨勇1, 丁长玲5, 左金华1*   

  1. 1. 滨州医学院附属医院口腔颌面外科 山东 滨州 256603;
    2. 滨州医学院附属医院检验科 山东 滨州 256603;
    3. 滨州医学院附属医院病理科 山东 滨州 256603;
    4. 滨州医学院附属医院口腔内科 山东 滨州 256603;
    5. 滨州医学院附属医院药学部 山东 滨州 256603
  • 收稿日期:2018-01-10 出版日期:2018-07-28 发布日期:2018-07-20
  • 通讯作者: 左金华,E-mail:jinhua256603@163.com
  • 作者简介:唐学敏(1989~ ),女,山东滨州人,硕士在读,主要 从事口腔颌面外科的研究工作。
  • 基金资助:
    山东省医药卫生科技发展计划(编号: 2015WS0487,2011HW004)

Expression of Aquaporin 5 and Programmed Cell Death 5 in Rat Parotid Gland after Ligation and Recanalization

TANG Xue-min1, SUN Chang-hua2, ZHU Yu-hong3, WANG Jing4 , YANG Yong1, DING Chang-ling5, ZUO Jin-hua1*   

  1. 1. Department of Oral and Maxillofacial Surgery, Affiliated Hospital of Binzhou Medical College, Binzhou 256603, China;
    2. Clinical Laboratory, Affiliated Hospital of Binzhou Medical College, Binzhou 256603, China;
    3. Department of Pathology, Affiliated Hospital of Binzhou Medical College, Binzhou 256603, China;
    4. Department of Oral Medicine, Affiliated Hospital of Binzhou Medical College, Binzhou 256603, China;
    5. Department of Pharmacy, Affiliated Hospital of Binzhou Medical College, Binzhou 256603, China.
  • Received:2018-01-10 Online:2018-07-28 Published:2018-07-20

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摘要： 目的:研究大鼠腮腺在不同的导管结扎时间点再通后的再生能力,探讨水通道蛋白5(aquaporin 5,AQP5)和细胞程序性死亡分子5(programmed cell death 5,PDCD5)在腮腺再生过程中的作用。方法:结扎大鼠腮腺主导管,分别在结扎7 d(A组)、结扎14 d(B组)、结扎21 d(C组)再通后取不同时间点(0、1、 3、 5、 7、 10、 14、 21、 28 d)腮腺标本,通过苏木素-伊红(hematoxylin and eosin stain,HE)染色观察腺体组织的形态变化,并通过免疫组织化学法、实时定量聚合酶链反应(real-time quantitative polymerase chain reaction,real-time PCR)等方法研究AQP5和PDCD5在腮腺中的表达规律。结果: 腮腺主导管结扎再通后3组均出现腺体再生现象,结扎7 d和14 d组的腮腺再通后AQP5表达逐渐增多,可分别在再通后第14天和第21天恢复至正常水平,而结扎21 d组,在再通28 d后仍未能完全恢复。PDCD5在腺体再通后随导管的凋亡出现增高趋势,3组分别在再通后第5天、第10天、第14天达到最高水平,B组峰值最大,C组其次,A组峰值最小。结论: 大鼠腮腺主导管结扎时间越久,其再生能力越差,AQP5的表达是腮腺组织功能恢复的重要指标,腺体再生过程中导管样结构发生凋亡。

关键词: 腮腺主导管再通, 水通道蛋白5, 细胞程序性死亡分子5, 免疫组织化学染色, 实时定量聚合酶链反应

Abstract: Objective: To investigate the regenerating ability of parotid gland after different ligated time, and the effect of aquaporin 5 (AQP5) and programmed cell death 5 (PDCD5) on the regeneration of parotid gland in rats. Methods: The parotid gland was ligated and then reopened after 7 days (group A),14 days (group B),and 21 days (group C),respectively.The samples were obtained at different time points (0,1,3,5,7,10,14,21, and 28 days). The morphological changes of glandular tissue were observed by HE staining, and the expression patterns of AQP5 and PDCD5 in acinor cells and glandular cells were explored. Results: Gland regeneration occurred in three groups after the reopen of main duct of parotid gland. The expression of AQP5 could increase to the normal level after reopen 14 days and 21 days for group A and group B, however, not recover to the normal level even after 28 days for group C. Expression of PDCD5 increased with the increase of apoptosis of the duct after the gland release. The three groups reached the highest level respectively after the recanalization of fifth days, the tenth day, and the fourteenth day, the peak value of group B was the highest, group C was the second, and the peak value of group A was the lowest. Conclusions: The longer the time ligation of the main duct of rat parotid gland, the worse the ability of regeneration. The expression of AQP5 is an important marker of functional recovers of the gland. Apoptosis of ductal like structure happened during the gland regeneration.

Key words: Parotid gland duct reopen, AQP5, PDCD5, Immunohistochemical, Real-time quantitative polymerase chain reaction