富血小板纤维蛋白对牙髓干细胞增殖和成骨分化的功能研究

doi:10.13701/j.cnki.kqyxyj.2020.10.019

口腔医学研究 ›› 2020, Vol. 36 ›› Issue (10): 973-977.DOI: 10.13701/j.cnki.kqyxyj.2020.10.019

富血小板纤维蛋白对牙髓干细胞增殖和成骨分化的功能研究

闫娜^*, 黄涛, 张中月, 张淑华, 王丽华, 韩国良

哈励逊国际和平医院口腔科河北衡水 053000

收稿日期:2020-03-23 出版日期:2020-10-28 发布日期:2020-10-19
通讯作者: *闫娜,E-mail:guchengchengpa@163.com
作者简介:闫娜(1985~ ),女,河北衡水人,硕士,主治医师,研究方向:口腔颌面外科。

Functions of Platelet-rich Fibrin on Proliferation and Osteogenic Differentiation of Dental Pulp Stem Cells

YAN Na^*, HUANG Tao, ZHANG Zhongyue, ZHANG Shuhua, WANG Lihua, HAN Guoliang

Department of Stomatology, Halixun International Peace Hospital, Hengshui 053000, China

Received:2020-03-23 Online:2020-10-28 Published:2020-10-19

摘要/Abstract

摘要： 目的: 探究富血小板纤维蛋白对人牙髓干细胞增殖和成骨分化的影响。方法: 使用不同稀释浓度的富血小板纤维蛋白处理人牙髓干细胞,通过MTT法检测人牙髓干细胞的增殖活性;流式细胞术检测人牙髓干细胞细胞周期变化;Western blot检测人牙髓干细胞成骨分化标志物表达情况。结果: 当富血小板纤维蛋白浓度达到50%以上,人牙髓干细胞增殖活性显著增强,更多细胞被诱导进入S期,成骨分化标志物ALP、COL-I和RUNX2表达显著上调,当富血小板纤维蛋白浓度达到80%,OCN表达也显著增加。结论: 富血小板纤维蛋白具有促进人牙髓干细胞增殖和成骨分化的作用。

关键词: 富血小板纤维蛋白, 人牙髓干细胞, 增殖, 成骨分化

Abstract: Objective: To investigate the effects of platelet-rich fibrin on the proliferation and osteogenic differentiation of human dental pulp stem cells. Methods: Human dental pulp stem cells were treated with platelet-rich fibrin of different dilution concentrations, and the proliferative activity of human dental pulp stem cells was detected by MTT assay. Flow cytometry was used to detect the cell cycle. The expression level of bone differentiation markers ALP, COL-I, RUNX2, and OCN were measured by western blot. Results: When the concentration of platelet-rich fibrin reached more than 50%, the proliferation activity of human pulp stem cells was significantly enhanced, and more cells were induced to S phase. The expression of osteogenic differentiation markers ALP, COL-I, and RUNX2 were significantly up-regulated. When the concentration of platelet-rich fibrin reached 80%, the expression of OCN was also significantly increased. Conclusion: Platelet-rich fibrin can promote the proliferation and osteogenic differentiation of human dental pulp stem cells.

Key words: platelet rich fibrin, human dental pulp stem cells, proliferation, osteogenic differentiation

闫娜, 黄涛, 张中月, 张淑华, 王丽华, 韩国良. 富血小板纤维蛋白对牙髓干细胞增殖和成骨分化的功能研究[J]. 口腔医学研究, 2020, 36(10): 973-977.

YAN Na, HUANG Tao, ZHANG Zhongyue, ZHANG Shuhua, WANG Lihua, HAN Guoliang. Functions of Platelet-rich Fibrin on Proliferation and Osteogenic Differentiation of Dental Pulp Stem Cells[J]. Journal of Oral Science Research, 2020, 36(10): 973-977.

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富血小板纤维蛋白对牙髓干细胞增殖和成骨分化的功能研究

Functions of Platelet-rich Fibrin on Proliferation and Osteogenic Differentiation of Dental Pulp Stem Cells

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