喷砂酸蚀对钛铌锆锡合金表面成骨细胞粘附、增殖和分化的影响

doi:10.13701/j.cnki.kqyxyj.2017.09.006

口腔医学研究 ›› 2017, Vol. 33 ›› Issue (9): 933-937.DOI: 10.13701/j.cnki.kqyxyj.2017.09.006

喷砂酸蚀对钛铌锆锡合金表面成骨细胞粘附、增殖和分化的影响

冯帅儒，刘鑫，潘爽，刘会梅，牛玉梅^*

哈尔滨医科大学口腔医学院牙体牙髓病科黑龙江哈尔滨 150001

收稿日期:2017-02-15 出版日期:2017-09-20 发布日期:2017-09-27
通讯作者: 牛玉梅,E-mail:yumeiniu@163.com
作者简介:冯帅儒(1990~ ),男,吉林省东丰县人,硕士,医师,主要从事牙体牙髓病的临床治疗工作。
基金资助:
黑龙江省卫生厅科研课题资助项目(编号:2011-051)

Effect of Sandblast and Acid Etching on Attachment, Proliferation, and Differentiation of Osteoblasts on Ti-24Nb-4Zr-7.9Sn.

FENG Shuai-ru, LIU Xin, PAN Shuang, LIU Hui-mei, NIU Yu-Mei^*

Department of Endodontics, Stomatology College of Harbin Medical University, Harbin 150001, China.

Received:2017-02-15 Online:2017-09-20 Published:2017-09-27

摘要/Abstract

摘要： 目的:研究喷砂酸蚀(SLA)对钛及钛铌锆锡合金(Ti-24Nb-4Zr-7.9Sn,TNZS)表面形貌的影响,观察合金的形貌学特征,评价其生物相容性。方法:将试样分为钛机械打磨并抛光组(Ti组),钛铌锆锡机械打磨并抛光组(TNZS组),钛喷砂酸蚀组(Ti-SLA组)和钛铌锆锡喷砂酸蚀组(TNZS-SLA组),共4组。通过扫描电镜观察各组试样的表面形貌,3D激光共聚焦显微镜和接触角测量仪测量各组试样表面的粗糙度与亲水性。接种MC3T3-E1小鼠前成骨细胞于各组试样表面,检测细胞在试样表面的粘附、增殖与矿化的能力,评估其生物相容性。结果:SLA处理后在材料表面形成纳米级及微米级的凹坑,产生均匀分布的粗糙结构,经过处理后材料仍保持亲水性。细胞在TNZS组上短期粘附明显高于其它组(P<0.05),TNZS-SLA组细胞增殖、分化能力均明显高于其它组(P<0.05)。结论:喷砂酸蚀后材料表面相对于光滑材料表面能更有效的促进成骨细胞在其表面增殖、分化,经喷砂酸蚀的钛铌锆锡合金具有良好的细胞相容性。

关键词: 喷砂酸蚀, MC3T3-E1, T-24Nb-4Zr-7.9Sn, 细胞增殖与分化

Abstract: Objective: To evaluate the effect of sandblast and acid etching treatments on attachment, proliferation, and differentiation of osteoblasts on Ti and Ti-24Nb-4Zr-7.9Sn (TNZS). Methods: Samples were divided into four groups: Ti group, Ti-SLA group, TNZS group, and TNZS-SLA group. Ti and TNZS groups were polished with different rough emery papers, Ti-SLA and TNZS-SLA groups were polished with different rough emery papers and sandblasted by Al2O3 particles and acid etched by mixture of hydrochloric acid and sulfuric acid. Surface morphology of the samples was analyzed by scanning electron microscope (SEM). Contact angles were examined by Tensiometer and Contact Angle Meter. Roughness of samples was examined by 3D laser scanning confocal microscope. MC3T3-E1 were seeded on the surface of four groups of samples and evaluated by different means. Results: Ti-SLA and TNZS-SLA surfaces exhibited micro-topographical and nano-topographical modifications and roughness distributed evenly. After treatment, four groups had similar hydrophilicity. The adhesion of MC3T3-E1 preosteoblasts showed that Ti and TNZS had higher attachment than SLA groups (P<0.05). TNZS-SLA showed the highest proliferation and differentiation (P<0.05). Conclusion: SLA surface treatment generates porous microstructures and nanostructures. It can enhance proliferation and differentiation of osteoblasts. TNZS alloy treated by sandblast and acid etching possesses good cytocompatibility.

Key words: Sandblast and acid etching, MC3T3-E1 , Ti-24Nb-4Zr-7.9Sn, Cell proliferation and differentiation

中图分类号:

R329.2

冯帅儒，刘鑫，潘爽，刘会梅，牛玉梅. 喷砂酸蚀对钛铌锆锡合金表面成骨细胞粘附、增殖和分化的影响[J]. 口腔医学研究, 2017, 33(9): 933-937.

FENG Shuai-ru, LIU Xin, PAN Shuang, LIU Hui-mei, NIU Yu-Mei. Effect of Sandblast and Acid Etching on Attachment, Proliferation, and Differentiation of Osteoblasts on Ti-24Nb-4Zr-7.9Sn.[J]. Journal of Oral Science Research, 2017, 33(9): 933-937.

参考文献

[1] Abdel-Hady Gepreel, M. and M. Niinomi, Biocompatibility of Ti-alloys for long-term implantation [J]. J Mech Behav Biomed Mater, 2013, 20(4)∶407-415
[2] Krishna, B.V., S. Bose, and A. Bandyopadhyay, Low stiffness porous Ti structures for load-bearing implants [J]. Acta Biomater, 2007, 3(6)∶997-1006
[3] Hao YL. Elastic deformation behaviour of Ti-24Nb-4Zr-7.9Sn for biomedical applications [J]. Acta Biomater, 2007, 3(2)∶277-286
[4] Bang SM. Osteoblastic and osteoclastic differentiation on SLA and hydrophilic modified SLA titanium surfaces [J]. Clin Oral Implants Res, 2014, 25(7)∶831-837
[5] Guo Y. The bone tissue compatibility of a new Ti35Nb2Ta3Zr alloy with a low Young's modulus [J]. Int J Mol Med, 2013, 31(3)∶689-697
[6] 高勃.铸模温度对牙科用钛铌锆锡合金铸流率影响的研究[J].口腔医学研究,2006,22(02)∶113-115
[7] 陈学林,肖月.贮存条件对喷砂酸蚀钛种植体表面成骨细胞活性的影响[J].口腔医学研究,2016,32(2)∶168-171
[8] Qu Z, et al. The initial attachment and subsequent behavior regulation of osteoblasts by dental implant surface modification [J]. J Biomed Mater Res A, 2007, 82(3)∶658-668
[9] Truong VK, et al. The influence of nano-scale surface roughness on bacterial adhesion to ultrafine-grained titanium [J]. Biomaterials, 2010, 31(13)∶3674-3683
[10] Yin C, et al. Effects of the micro-nano surface topography of titanium alloy on the biological responses of osteoblast [J]. J Biomed Mater Res A, 2017, 105(3)∶757-769
[11] Brett PM, et al. Roughness response genes in osteoblasts [J]. Bone, 2004. 35(1)∶124-133
[12] 陆斌.TiO₂喷砂酸蚀处理对钛片表面人成骨细胞BMP-2表达水平的影响[J].山西医科大学学报,2013(05)∶358-360
[13] Sista S, et al. The influence of surface energy of titanium-zirconium alloy on osteoblast cell functions in vitro [J]. J Biomed Mater Res A, 2011, 97(1)∶27-36

喷砂酸蚀对钛铌锆锡合金表面成骨细胞粘附、增殖和分化的影响

Effect of Sandblast and Acid Etching on Attachment, Proliferation, and Differentiation of Osteoblasts on Ti-24Nb-4Zr-7.9Sn.

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 8

编辑推荐

Metrics

[1]	王松松, 马向瑞, 张云涛. 桂皮醛对高糖环境下MC3T3-E1成骨细胞增殖与分化的影响[J]. 口腔医学研究, 2019, 35(7): 712-715.
[2]	张宇, 侯玉东, 张云涛, 杨忠学, 乔鲁卉. HU-308对高浓度糖皮质激素作用下MC3T3-E1成骨细胞生物学性能的影响[J]. 口腔医学研究, 2019, 35(1): 98-102.
[3]	曾浩, 王敏, 陈冬, 施斌, 白轶. 选择性激光烧结技术制作的双相磷酸钙骨组织工程支架的工艺和生物学性能[J]. 口腔医学研究, 2018, 34(2): 165-168.
[4]	曹亚洲, 程伟, 胡勤刚. 钛表面RGD/仿生磷酸钙复合涂层对MC3T3-E1细胞黏附、增殖、分化的影响[J]. 口腔医学研究, 2017, 33(7): 693-697.
[5]	陈学林,肖月. 贮存条件对喷砂酸蚀钛种植体表面成骨细胞活性的影响[J]. 口腔医学研究, 2016, 32(2): 168-171.
[6]	安佰利,李至睿,刁晓鸥,辛海涛,郭亚洲,吴玉禄. 喷砂酸蚀对超细晶纯钛表面MC3T3-E1细胞粘附与增殖的影响[J]. 口腔医学研究, 2016, 32(1): 26-30.
[7]	张宇, 马肃, 刘培红, 秦春林. 硫酸软骨素蛋白聚糖对MC3T3-E1细胞株矿化的影响[J]. 口腔医学研究, 2015, 31(4): 317-319.
[8]	高媛媛, 周子谦, 柳慧芬, 童昕. RGD多肽修饰的无机小牛骨粉对MC3T3-E1细胞黏附、增殖及分化的影响[J]. 口腔医学研究, 2015, 31(4): 354-358.