口腔医学研究

口腔医学研究 ›› 2019, Vol. 35 ›› Issue (1): 98-102.DOI: 10.13701/j.cnki.kqyxyj.2019.01.023

• 口腔生物学研究 • 上一篇    

HU-308对高浓度糖皮质激素作用下MC3T3-E1成骨细胞生物学性能的影响

张宇, 侯玉东*, 张云涛, 杨忠学, 乔鲁卉   

  1. 滨州医学院 山东 烟台 264003
  • 收稿日期:2018-07-13 出版日期:2019-01-18 发布日期:2019-01-28
  • 通讯作者: 侯玉东,E-mail:bycgk@126.com
  • 作者简介:张宇(1993~ ),男,山东淄博人,硕士在读,研究方向为口腔种植修复。

Effects of HU-308 on Biological Properties of MC3T3-E1 Osteoblasts at High Glucocorticoids Concentrations

ZHANG Yu, HOU Yu-dong*, ZHANG Yun-tao, YANG Zhong-xue, QIAO Lu-hui   

  1. Binzhou Medical University, Yantai 264003, China
  • Received:2018-07-13 Online:2019-01-18 Published:2019-01-28

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摘要： 目的:观察HU-308对高浓度糖皮质激素作用下成骨细胞生物学性能的影响。方法:将MC3T3-E1细胞在高浓度糖皮质激素(10-6 mmol/L)进行培养,按HU-308浓度分为0(对照组)、10-10、10-9、10-8、10-7、10-6 mol/L(实验组)6组,培养1、4、7 d检测细胞增殖及碱性磷酸酶(alkaline phosphatase,ALP)活性、成骨基因特异性转录因子Runt相关基因2(runt related transcription factor-2,RUNX2)及护骨素(osteoprotegerin,OPG) mRNA的表达,培养24 h观察细胞骨架的形态变化。结果:细胞计数试剂盒(cell counting kit-8,CCK-8)结果显示,对照组与实验组相比差异均有统计学意义(P<0.05),培育4 d,各实验组比较,药物浓度增加对细胞增殖的促进作用越明显(P<0.05)。ALP活性检测结果显示,实验组与对照组比较差异无统计学意义(P>0.05)。细胞骨架染色随HU-308浓度的增高细胞铺展面积增大且内部框架结构更加清晰。低浓度组Runx2、OPG mRNA表达水平明显上调(P<0.05);而高浓度组Runx2、OPG mRNA的表达受到抑制(P<0.05)。结论:在高浓度糖皮质激素作用下,HU-308可促进MC3T3-E1细胞的粘附、伸展与增殖,且低浓度HU-308可促进其Runx2、OPG mRNA的表达。

关键词: HU-308, 糖皮质激素, MC3T3-E1, 增殖

Abstract: Objective: To observe the effects of HU-308 on osteoblast treated with high concentration of glucocorticoid. Methods: MC3T3-E1 cells were cultured in the high concentration of glucocorticoid (10-6 mmol/L) and then added the HU-308. Cells in concentration of 10-10, 10-9, 10-8, 10-7, and 10-6 mol/L of HU-308 were set as the experimental groups, and that with only glucocorticoid was set as the control group. The CCK-8 was used to detect the cell proliferation, ALP activity, and mRNA expression of Runx2 and OPG. The morphology of the cytoskeleton was observed by confocal laser scanning microscope when the MC3T3-E1 cells were cultured for 24h. Results: The results of CCK-8 showed that there was statistically significant between the control group and experimental group (P<0.05). The cell proliferation was effectively promoted in the experimental group (10-9, 10-8, 10-7 and 10-6 mol/L) in 4 d (P<0.05). ALP activity detection showed no significant difference between experimental groups and control group (P>0.05). With the concentration of HU-308 increased, cells spreading area increased and the internal frame structure was clearer. The mRNA expressions of Runx2 and OPG were significantly raised at low concentration of HU-308 (P<0.05), however, inhibited at high concentration (P<0.05). Conclusion: HU-308 promoted the proliferation, adhesion, and extension of MC3T3-E1 cells under the action of high concentration of glucocorticoids, and low concentration of HU-308 could promote the expression of Runx2 and OPG mRNA.

Key words: HU-308, Glucocorticoids, MC3T3-E1, Proliferation