桂皮醛对高糖环境下MC3T3-E1成骨细胞增殖与分化的影响

doi:10.13701/j.cnki.kqyxyj.2019.07.022

口腔医学研究 ›› 2019, Vol. 35 ›› Issue (7): 712-715.DOI: 10.13701/j.cnki.kqyxyj.2019.07.022

桂皮醛对高糖环境下MC3T3-E1成骨细胞增殖与分化的影响

王松松, 马向瑞, 张云涛^*

滨州医学院附属医院口腔科山东滨州 256600

收稿日期:2018-12-10 出版日期:2019-07-25 发布日期:2019-07-24
通讯作者: 张云涛,E-mail:zytao999@163.com
作者简介:王松松(1993~),男,山东滨州人,硕士在读,研究方向为口腔种植修复
基金资助:
山东省医药卫生科技计划项目(编号:2017WS231)

Effects of Cinnamaldehyde on Proliferation and Differentiation of MC3T3-E1 Osteoblasts Induced by High Glucose

WANG Songsong, MA Xiangrui, ZHANG Yuntao^*

Department of Prosthodontics, Affiliated Hospital to Binzhou Medical University, Binzhou 256600, China.

Received:2018-12-10 Online:2019-07-25 Published:2019-07-24

摘要/Abstract

摘要： 目的:观察桂皮醛对高糖作用下MC3T3-E1成骨细胞增殖与分化的影响。方法:用 0(对照组)、10、20、40 mg/L的桂皮醛分别在葡萄糖(25 mmol/L)的环境下培养MC3T3-E1细胞,培养 1、4、7 d后检测细胞增殖、碱性磷酸酶活性,茜素红染色观察钙化结节,鬼笔环肽染色观察细胞结构框架以及成骨相关基因骨钙蛋白(osteocalcin,OCN) mRNA表达水平。结果:桂皮醛可促进高糖环境下成骨细胞增殖及ALP活性(P<0.05);矿化结节数量增多且体积增大;实验组OCN mRNA表达水平明显上调(P<0.05);细胞骨架染色结果示:当桂皮醛浓度为10、20 mg/L时细胞铺展面积增大且细胞骨架更加清晰。结论:桂皮醛可促进高糖作用下MC3T3-E1成骨细胞的增殖与分化

关键词: 桂皮醛, MC3T3-E1, 骨钙蛋白, 增殖

Abstract: Objective: To observe the effects of cinnamaldehyde (CA) on the proliferation and differentiation of MC3T3-E1 osteoblasts induced by high glucose. Methods: MC3T3-E1 cells were cultured in glucose (25 mmol/L) with 0 (control), 10, 20, and 40 mg/L CA. Cell proliferation, ALP activity, and alizarin red staining was measured. Morphology of the cytoskeleton was observed by confocal laser scanning microscope. Expression levels of osteogenesis related genes OCN mRNA were detected by real-time PCR. Results: CA could promote the proliferation and ALP activity of osteoblasts in high glucose environment (P<0.05). The number and the volume of mineralized nodules increased. The expression of OCN mRNA in the experimental group was significantly up-regulated (P<0.05). When the concentration of CA was 10mg/L and 20mg/L, the cell spreading area was increased and the cytoskeleton was clearer. Conclusion: CA can promote the proliferation and differentiation of MC3T3-E1.

Key words: Cinnamaldehyde, MC3T3-E1 , OCN, Proliferation

王松松, 马向瑞, 张云涛. 桂皮醛对高糖环境下MC3T3-E1成骨细胞增殖与分化的影响[J]. 口腔医学研究, 2019, 35(7): 712-715.

WANG Songsong, MA Xiangrui, ZHANG Yuntao. Effects of Cinnamaldehyde on Proliferation and Differentiation of MC3T3-E1 Osteoblasts Induced by High Glucose[J]. Journal of Oral Science Research, 2019, 35(7): 712-715.

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桂皮醛对高糖环境下MC3T3-E1成骨细胞增殖与分化的影响

Effects of Cinnamaldehyde on Proliferation and Differentiation of MC3T3-E1 Osteoblasts Induced by High Glucose

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