贮存条件对喷砂酸蚀钛种植体表面成骨细胞活性的影响

doi:10.13701/j.cnki.kqyxyj.2016.02.016

口腔医学研究 ›› 2016, Vol. 32 ›› Issue (2): 168-171.DOI: 10.13701/j.cnki.kqyxyj.2016.02.016

贮存条件对喷砂酸蚀钛种植体表面成骨细胞活性的影响

陈学林肖月^*

佳木斯大学附属第二医院口腔医院修复一科,佳木斯大学黑龙江佳木斯 154007

收稿日期:2015-08-04 出版日期:2016-02-28 发布日期:2016-03-10
通讯作者: 肖月,E-mail:15765341826@163.com
作者简介:陈学林(1989~),男,黑龙江人,硕士在读,主要从事口腔修复学临床及基础的研究工作。
基金资助:
佳木斯大学研究生科技创新项目(编号:LM2015_073)

Effect of Storage Condition on the Osteoblasts Activity on Sandblasted and Acid-etched Titanium Surfaces.

CHEN Xue-lin, XIAO Yue.

Department of Prosthodontics, Second Affiliated Hospital of Jiamusi University,Jiamusi 154007, China

Received:2015-08-04 Online:2016-02-28 Published:2016-03-10

摘要/Abstract

摘要： 目的:研究不同贮存条件对与喷砂酸蚀钛种植体表面成骨细胞黏附、增殖等影响。方法:采用喷砂酸蚀技术对钛片进行改性,并将钛片分别放入组A密闭容器、组B双蒸水(ddH₂O)、组C氢氧化钠(NaOH)中保存4周。将成骨细胞接种到钛片表面,通过细胞形态观察,黏附增殖检测、碱性磷酸酶活性检测,分析3种贮存方法对种植体的影响。结果:在3 h、1 d、4 d、7 d,成骨细胞在钛片上黏附增殖具有统计学意义(p<0.05)。在培养7 d后组C的ALP活性大于组A大于组B,差异具有统计学意义(p<0.05)。培养24 h时组C细胞伸展良好,有大量伪足向四周伸展。结论:液体中保存可以减少种植体活性丧失,在NaOH中保存效果最好。

关键词: 纯钛, 喷砂酸蚀, 贮存条件, 成骨细胞

Abstract: Objective: To investigate the effects of different storage methods on adhesion and proliferation rate of osteoblasts on the surface of titanium implant. Methods: The titanium plates were modified with sandblasted and acid-etching technology and divided into three groups according to the storage methods. Group A was in the airtight container, group B was in the steaming water (ddH2O), and group C was in the sodium hydroxide (NaOH) solution for 4 weeks. Adhesion and proliferation of osteoblasts at different time points on the surface of titanium were evaluated by MTT assay, alkaline phosphatase activity assay, and laser scanning confocal microscope. Results: After 1 hour and 3 hours culture, the number of cells on group B and group C surface were higher than that on group A (P<0.05). After 1, 4, 7 days culture, the number of cells on group C was higher than those on other two groups (P<0.05). Under laser scanning confocal microscope, osteoblasts were found to spread better on group C surface. After 7 days culture, alkaline phosphatase activity of cells on group C was higher than those on group B and group A (P<0.05). Conclusion: The titanium plates preserved in the liquid was beneficial to maintain the biological activity. Sodium hydroxide solution might be the optimal way for implant storage.

中图分类号:

R783.1

陈学林,肖月. 贮存条件对喷砂酸蚀钛种植体表面成骨细胞活性的影响[J]. 口腔医学研究, 2016, 32(2): 168-171.

CHEN Xue-lin, XIAO Yue.. Effect of Storage Condition on the Osteoblasts Activity on Sandblasted and Acid-etched Titanium Surfaces.[J]. Journal of Oral Science Research, 2016, 32(2): 168-171.

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贮存条件对喷砂酸蚀钛种植体表面成骨细胞活性的影响

Effect of Storage Condition on the Osteoblasts Activity on Sandblasted and Acid-etched Titanium Surfaces.

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