口腔医学研究

口腔医学研究 ›› 2018, Vol. 34 ›› Issue (6): 609-613.DOI: 10.13701/j.cnki.kqyxyj.2018.06.009

• 口腔骨生物学研究 • 上一篇    下一篇

纯钛表面载环肽c(RGDfk)对类成骨细胞增殖和信号转导通路的影响

全雨坚, 李德超*, 李慕勤*, 张慧明, 刘洋   

  1. 佳木斯大学 黑龙江省高校生物医学材料重点实验室 黑龙江 佳木斯 154007
  • 收稿日期:2017-12-21 出版日期:2018-06-20 发布日期:2018-06-21
  • 通讯作者: 李慕勤,E-mail: jmsdxlimuqin@163.com; 李德超,E-mail:dechaoli2004@yahoo.com.cn
  • 作者简介:全雨坚(1992~ ),女,广西壮族自治区贺州市人,硕士,主要从事口腔种植学研究工作。
  • 基金资助:
    国家自然科学基金(编号:31370979);佳木斯大学口腔医学预防与治疗协同创新中心(编号:2011xtcx2016-02)

Effect of Pure Titanium Suface-loaded Cyclo (RGDfk) on Proliferation and Signal Transduction Pathway of Osteoblasts

QUAN Yu-jian, LI De-chao*, LI Mu-qin*, ZHANG Hui-ming, LIU Yang   

  1. Key Biomedical Materials Laboratory of Colleges and Universities in Heilongjiang Province, Jiamusi University, Jiamusi 154007, China
  • Received:2017-12-21 Online:2018-06-20 Published:2018-06-21

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摘要： 目的:通过纯钛表面微弧氧化(MAO),以多巴胺为耦联,引入c(RGDfk) 环肽,研究体外类成骨细胞在该涂层上的增殖及细胞活性转导信号通路的影响。方法:通过CCK-8法、ALP法分析MC3T3-E1成骨细胞株在不同涂层上的细胞增殖成骨的影响;应用Western blot法,分析MC3T3-E1在不同涂层培养后的黏着斑激酶信号通路及MAPK信号通路上的相关蛋白量与活性。结果:细胞的增殖和活性在纯钛表面微弧氧化-多巴胺-环肽c(RGDfk)复合涂层影响明显,优于纯钛微弧氧化-多巴胺涂层,更优于纯钛微弧氧化涂层;细胞在该复合涂层培养后上调了黏着斑激酶及MAPK信号通路上的相关蛋白量与活性。结论:MC3T3-E1成骨细胞在纯钛表面微弧氧化-多巴胺-c(RGDfk)环肽复合涂层培养后,增殖明显,黏着斑激酶信号通路与MAPK信号通路相关蛋白表达增加。

关键词: 环肽c(RGDfk), 信号通路, 纯钛, 微弧氧化, 成骨细胞

Abstract: Objective: To study the proliferation and cell viability of osteoblasts in vitro by introducing microencapsulated MAO and dopamine into c(RGDfk) cyclic peptide. Methods: The effects of MC3T3-E1 osteoblasts proliferation and osteogenesis were detected by CCK-8 assay and ALP assay. Western blotting was used to analyze the focal adhesion kinase signal pathway and MAPK signaling pathway related protein content of MC3T3-E1 cells on different coatings. Results: The cell proliferation and activity were significantly affected by microarc oxidation-dopamine-cyclic peptide c (RGDfk) coating on pure titanium surface, which was better than those on pure titanium micro-arc oxidation-dopamine coating. The focal adhesion kinase and MAPK signaling pathway related protein levels and activity of cells in the composite coating were upregulated. Conclusion: The proliferation and signal transduction pathway of MC3T3-E1 osteoblasts were enhanced on the surface of pure titanium after microarc oxidation-dopamine-c (RGDfk) cyclic peptide coating.

Key words: Cyclic peptide c(RGDfk), Signal Pathway, Pure Titanium, Microarc Oxidation, Osteoblast