口腔医学研究

口腔医学研究 ›› 2018, Vol. 34 ›› Issue (6): 605-608.DOI: 10.13701/j.cnki.kqyxyj.2018.06.008

• 口腔骨生物学研究 • 上一篇    下一篇

N-Ac-L-Leu-PEI/微小RNA-34a复合物对大鼠颅骨缺损成骨的影响

胡天琦1,2, 申玉芹1, 费鸿博1,2, 李洋洋1,2, 顾中一1,2, 毕雪婷1,2, 王卓然1,2, 李文洁1,2, 林崇韬1*   

  1. 1. 吉林大学口腔医院牙周科 吉林 长春 130021;
    2. 吉林大学口腔医院 吉林省牙发育及颌骨重塑与再生重点实验室 吉林 长春 130021
  • 收稿日期:2017-09-16 出版日期:2018-06-20 发布日期:2018-06-21
  • 通讯作者: 林崇韬,E-mail:linct@jlu.edu.cn
  • 作者简介:胡天琦(1991~ ),女,吉林人,硕士,医师,主要从事牙周病学研究工作。
  • 基金资助:
    国家自然科学基金面上项目(编号:81371153);吉林省教育厅项目(吉教科合字[2016]485号);吉林省科技发展计划自然科学基金项目(编号:20160101335JC);吉林省省级医药健康产业发展专项资金项目(编号:20170311032YY);吉林省科技发展计划自然科学基金项目(编号:20150101173JC)

Effects of N-Ac-L-Leu-PEI/microRNA-34a Complex on Osteogenisis of Rat Cranial Defects

HU Tian-qi1,2, SHEN Yu-qin1, FEI Hong-bo1,2, LI Yang-yang1,2, GU Zhong-yi1,2, BI Xue-ting1,2, WANG Zhuo-ran1,2, LI Wen-jie1,2, LIN Chong-tao1*   

  1. 1. Department of Periodontology, Stomatology Hospital, Jilin University, Changchun 130021, China;
    2. Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling, Department of Periodontology, Stomatology Hospital, Jilin University, Changchun 130021, China
  • Received:2017-09-16 Online:2018-06-20 Published:2018-06-21

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摘要： 目的:将N-Ac-L-Leu-PEI/微小RNA(microRNA,miR)-34a复合物用于大鼠颅骨缺损模型,探讨miR-34a对骨缺损区成骨的影响。方法:Wistar雄性大鼠32只,制备标准大鼠颅骨缺损模型,随机分为4组,分别于缺损区覆盖载有磷酸盐缓冲液 (phosphate buffered salin, PBS)、N-Ac-L-Leu-PEI、N-Ac-L-Leu-PEI/miR-34a复合物、N-Ac-L-Leu-PEI/miR-34a NC复合物的口腔生物膜,饲养10周后处死大鼠,通过锥形束CT (cone beam computerized tomography,CBCT)、微型CT (microcomputed tomography,micro-CT)、反转录-聚合酶链反应(reverse transcription-polymerase chain reaction, RT-PCR)等方法检测大鼠颅骨缺损区新骨形成情况。结果:N-Ac-L-Leu-PEI/miR-34a复合物组缺损区骨新生最为明显,新生骨量与总组织量之比 (bone volume / total volum, BV/TV)、骨小梁厚度 (trabecular thickness, Tb.Th)值均高于其它组,成骨标志性基因Runt相关转录因子2 ( runt-related transcription factor 2, Runx2)、Osterix转录因子 (serine protease 7, SP7)及Ⅰ型胶原 (Collagen Ⅰ, ColⅠ)的mRNA表达显著上调。结论:对实验性大鼠颅骨缺损模型,N-Ac-L-Leu-PEI/miR-34a具有促进缺损区成骨的作用。

关键词: 微小RNA, N-Ac-L-Leu-PEI, N-Ac-L-Leu-PEI/miR-34a复合物, 大鼠颅骨缺损模型

Abstract: Objective: To study the effects of N-Ac-L-Leu-PEI/micro RNA(miR)-34a complex on osteogenisis of rat cranial defects. Methods: Thirty-two Wistar male rats were randomly divided into four groups and standard cranial defects were prepared on each rat. Biofilms loaded with PBS, N-Ac-L-Leu-PEI, N-Ac-L-Leu-PEI/miR-34a complex, and N-Ac-L-Leu-PEI/miR-34a NC complex covered the defect. After ten weeks culture, the rats were sacrificed and the development of osteogenisis was detected by CBCT, micro-CT and RT-PCR. Results: Bone formation was evident in the defects of N-Ac-L-Leu-PEI / miR-34a complex group. The BV/TV values and Tb.Th values of N-Ac-L-Leu-PEI/miR-34a complex group increased significantly, suggesting new bone formation. The expression of Runx2, SP7, and Col I mRNA was up-regulated by N-Ac-L-Leu-PEI/miR-34a complex, which were specific factors of osteogenisis. Conclusion: N-Ac-L-Leu-PEI/miR-34a complex can promote osteogenesis in rat cranial defects.

Key words: miR-34a, N-Ac-L-Leu-PEI, N-Ac-L-Leu-PEI/miR-34a complex, Rat cranial defects