微小RNA-210-3p在低氧环境中对人牙髓细胞增殖和分化的影响

doi:10.13701/j.cnki.kqyxyj.2019.10.015

口腔医学研究 ›› 2019, Vol. 35 ›› Issue (10): 970-974.DOI: 10.13701/j.cnki.kqyxyj.2019.10.015

微小RNA-210-3p在低氧环境中对人牙髓细胞增殖和分化的影响

袁晓琴^*, 奂忠平, 王洪涛

三峡大学第一临床医学院,宜昌市中心人民医院口腔科湖北宜昌 443000

收稿日期:2019-04-12 出版日期:2019-10-28 发布日期:2019-10-22
通讯作者: 袁晓琴,E-mail:94473737@qq.com
作者简介:袁晓琴(1983~ ),女,湖北五峰人,硕士,主治医师,主要从事口腔修复研究。

Effects of microRNA-210-3p on Proliferation and Differentiation of Human Dental Pulp Cells under Hypoxic Condition.

YUAN Xiaoqin^*, HUAN Zhongping, WANG Hongtao

Department of Stomatology, The First College of Clinical Medical Science, China Three Gorges University, Yichang Central People's Hospital, Yichang 443000, China.

Received:2019-04-12 Online:2019-10-28 Published:2019-10-22

摘要/Abstract

摘要： 目的:探讨微小RNA(microRNA,miR)-210-3p在低氧环境下对人牙髓细胞增殖和分化的影响。方法:分离提取人牙髓细胞,检测miR-210-3p在常氧环境和低氧环境下人牙髓细胞中的表达情况。转染miR-210-3p inhibitors至人牙髓细胞,细胞计数试剂盒(cell counting kit-8,CCK-8)检测miR-210-3p抑制对低氧环境下人牙髓细胞增殖的影响。碱性磷酸酶染色、实时荧光定量聚合酶链反应 (Real-time polymerase chain reaction,Real-time PCR)及茜素红染色检测抑制miR-210-3p表达对低氧环境下人牙髓细胞分化的调控作用。结果:低氧环境培养的人牙髓细胞高表达miR-210-3p,miR-210-3p在低氧环境下可以促进人牙髓细胞的增殖,并且miR-210-3p对低氧环境下的人牙髓细胞的早期分化具有促进作用,而对晚期矿化则起到抑制作用。结论:miR-210-3p参与调控低氧环境下人牙髓细胞的增殖和分化。

关键词: 微小RNA -210-3p, 低氧, 牙髓细胞, 增殖, 分化

Abstract: Objective: To evaluate the effects of miR-210-3p on proliferation and differentiation of human dental pulp cells under hypoxic condition. Methods: Human dental pulp cells were isolated, and the expression of miR-210-3p was detected in human dental pulp cells under normoxia and hypoxic condition. After transfection of miR-210-3p inhibitors into human dental pulp cells, the proliferation effect under hypoxia was measured by CCK-8 assay. Alkaline phosphatase staining, real-time PCR and alizarin red staining were adopted to evaluate the regulatory effects of miR-210-3p inhibition on differentiation of human dental pulp cells under hypoxic condition. Results: Human dental pulp cells cultured under hypoxic condition were highly expressed with miR-210-3p. In addition, miR-210-3p promoted proliferation and early differentiation of human dental pulp cells under hypoxia while the late differentiation was inhibited by miR-210-3p in low oxygen. Conclusion: miR-210-3p involves in the regulation of proliferation and differentiation in human dental pulp cells under hypoxic condition.

Key words: microRNA-210-3p, hypoxia, human dental pulp cells, proliferation, differentiation

袁晓琴, 奂忠平, 王洪涛. 微小RNA-210-3p在低氧环境中对人牙髓细胞增殖和分化的影响[J]. 口腔医学研究, 2019, 35(10): 970-974.

YUAN Xiaoqin, HUAN Zhongping, WANG Hongtao. Effects of microRNA-210-3p on Proliferation and Differentiation of Human Dental Pulp Cells under Hypoxic Condition.[J]. Journal of Oral Science Research, 2019, 35(10): 970-974.

参考文献

[1] 任璇,张志民,李玉娇.人牙髓干细胞低氧培养的研究进展[J].口腔生物医学,2017,8(4): 219-22.
[2] Mohr AM, Mott JL. Overview of microRNA biology [J]. Semin Liver Dis, 2015, 35(1): 3-11.
[3] Ren CX, Leng RX, Fan YG, et al. MicroRNA-210 and its theranostic potential [J]. Expert Opin Ther Targets, 2016, 20(11): 1325-1338.
[4] Bavelloni A, Ramazzotti G, Poli A, et al. MiRNA-210: a current overview [J]. Anticancer Res, 2017, 37(12):6511-6521.
[5] Dai Y, Lin X, Xu W, et al. MiR-210-3p protects endometriotic cells from oxidative stress-induced cell cycle arrest by targeting BARD1 [J]. Cell Death Dis, 2019, 10(2): 144.
[6] 曹铮, 冯林, 冯晓莉. 缺氧相关的miRNA-210在肿瘤中的研究进展[J].肿瘤防治研究,2018,45(7):500-504.
[7] 卢怡, 彭友俭. MicroRNA在口腔疾病中的研究进展[J].临床口腔医学杂志,2018,34(4):247-250.
[8] Ren D, Yang Q, Dai Y, et al. Oncogenic miR-210-3p promotes prostate cancer cell EMT and bone metastasis via NF-kappaB signaling pathway [J]. Mol Cancer, 2017, 16(1): 117.
[9] 韩兴涛,杨凌博,魏澎涛,等.miR-210-3p通过靶向ATG7抑制人膀胱癌细胞J82的自噬和诱导凋亡[J].中国临床解剖学杂志,2018,36(6): 615-620.
[10] Hu B, Li Y, Wang M, et al. Functional reconstruction of critical-sized load-bearing bone defects using a Sclerostin-targeting miR-210-3p-based construct to enhance osteogenic activity [J]. Acta Biomater, 2018, 76: 275-282.
[11] 杜小颖,丁威薇,陈悦,等.IGF-1通过PI3K/AKT信号通路促进牙髓细胞增殖及碱性磷酸酶活性的研究[J].口腔医学研究, 2017, 33(5): 538-541.
[12] Jin R, Song G, Chai J, et al. Effects of concentrated growth factor on proliferation, migration, and differentiation of human dental pulp stem cells in vitro [J]. J Tissue Eng, 2018, 9: 2041731418817505.
[13] 卢皙, 刘上峰, 赵守亮. GLUD1基因对人牙髓干细胞的体外增殖、分化和矿化的影响[J].上海口腔医学,2017,26(4): 358-362.
[14] Kim JY, Kim DS, Auh QS, et al. Role of protein phosphatase 1 in angiogenesis and odontoblastic differentiation of human dental pulp cells [J]. J Endod, 2017, 43(3): 417-424.
[15] 孙艳艳,胡伟平,刘宗响,等.Wnt3a蛋白对牙髓干细胞成骨向分化的影响[J].中华口腔医学杂志,2017,52(7): 427-431.
[16] 任璇.缺氧对人牙髓细胞线粒体及miR-210-3p表达的影响[D].吉林大学,2018.

微小RNA-210-3p在低氧环境中对人牙髓细胞增殖和分化的影响

Effects of microRNA-210-3p on Proliferation and Differentiation of Human Dental Pulp Cells under Hypoxic Condition.

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