TNF-α增强牙髓干细胞与脐静脉内皮细胞共培养血管生成能力

doi:10.13701/j.cnki.kqyxyj.2019.10.014

口腔医学研究 ›› 2019, Vol. 35 ›› Issue (10): 966-969.DOI: 10.13701/j.cnki.kqyxyj.2019.10.014

TNF-α增强牙髓干细胞与脐静脉内皮细胞共培养血管生成能力

安莉, 沈帅, 王璐瑶, 李勇, 牛玉梅, 潘爽^*

哈尔滨医科大学口腔医学院黑龙江哈尔滨 150001

收稿日期:2019-01-09 出版日期:2019-10-28 发布日期:2019-10-22
通讯作者: 潘爽,E-mail:panshuang@hrbmu.edu.cn
作者简介:安莉(1992~ ),女,辽宁辽阳人,硕士,主要研究方向为牙髓再生及牙髓血运重建等。
基金资助:
国家自然科学基金(编号:81570963)

TNF-α Increasing the Angiogenic Potential of Dental Pulp Cells and Endothelial Cells in Co-culture System.

AN Li, SHEN Shuai, WANG Luyao, LI Yong, NIU Yumei, PAN Shuang^*

Affiliated Stomatological Hospital of Harbin Medical University, Harbin 150001, China

Received:2019-01-09 Online:2019-10-28 Published:2019-10-22

摘要/Abstract

摘要： 目的:研究TNF-α对人牙髓干细胞和人脐静脉内皮细胞共培养体系体外血管生成能力的影响。方法:体外培养hDPSCs,并与HUVECs按1∶5比例行体外共培养。用0、50 μg/L TNF-α作用于共培养系统并进行体外血管形成实验,分别在3、6、9 h检测各组小管形成的相对长度和节点数目并行细胞活死染色,CCK8法检测50 μg/L TNF-α对共培养系统中两种细胞增殖的影响,采用SPSS 20.0软件对数据进行统计学分析。结果:体外小管形成实验结果显示在3、6、9 h,50 μg/L TNF-α作用的实验组形成的小管相对长度和分支点数明显高于空白对照组(P<0.05)。CCK8结果表明50 μg/L TNF-α对共培养组细胞的增殖与对照组相比,无明显变化(P>0.05)。结论:50 μg/L TNF-α增强人牙髓干细胞与人脐静脉内皮细胞共培养体系体外血管生成能力。

关键词: 牙髓干细胞, 脐静脉内皮细胞, 牙髓再生, 血管生成

Abstract: Objective: To investigate the effects of human dental pulp stem cells (hDPSCs) and human umbilical vein endothelial cells (HUVECs) in the co-culture system on formation of endothelial tubules and vascular networks. Methods: hDPSCs were co-cultured with HUVECs at a ratio of 1∶5. Matrigel assay were performed on the co-culture system to detect the total branching lengths and the number of branches. CCK-8 assay was used to examine the effect of TNF-α on the cell proliferation. LIVE/DEAD viability/cytotoxicity kit was used to detect the mammalian cells. Results: Matrigel assay showed the total branching length. The number of branches formed in the experimental groups treated with 50 μg/L TNF-α was significantly higher than that of the control group. The decomposition time of the tubule structures was significantly prolonged (P<0.05). After treated with 50 μg/L TNF-α, there was no significant changes in the proliferation of co-cultured cells (P>0.05) . Conclusion: TNF-α enhanced the angiogenic ability of hDPSCs and HUVECs in the co-culture system in vitro.

Key words: dental pulp stem cell, human umbilical vein endothelial cells, dental pulp regeneration, angiogenesis

安莉, 沈帅, 王璐瑶, 李勇, 牛玉梅, 潘爽. TNF-α增强牙髓干细胞与脐静脉内皮细胞共培养血管生成能力[J]. 口腔医学研究, 2019, 35(10): 966-969.

AN Li, SHEN Shuai, WANG Luyao, LI Yong, NIU Yumei, PAN Shuang. TNF-α Increasing the Angiogenic Potential of Dental Pulp Cells and Endothelial Cells in Co-culture System.[J]. Journal of Oral Science Research, 2019, 35(10): 966-969.

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TNF-α增强牙髓干细胞与脐静脉内皮细胞共培养血管生成能力

TNF-α Increasing the Angiogenic Potential of Dental Pulp Cells and Endothelial Cells in Co-culture System.

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