口腔医学研究

口腔医学研究 ›› 2019, Vol. 35 ›› Issue (4): 401-404.DOI: 10.13701/j.cnki.kqyxyj.2019.04.023

• 口腔生物学研究 • 上一篇    下一篇

骨髓间充质干细胞来源的外泌体对成骨细胞增殖和分化的影响

陈燕*, 姜胜军, 彭友俭   

  1. 武汉大学人民医院口腔科 湖北 武汉 430060
  • 收稿日期:2018-08-31 出版日期:2019-04-28 发布日期:2019-04-23
  • 通讯作者: 陈燕,E-mail:245094583@qq.com
  • 作者简介:陈燕(1983~ ),女,福建福州人,硕士,主治医师,主要研究方向为口腔修复种植学。
  • 基金资助:
    武汉大学青年教师资助项目(编号:2042018kf0145)

Effects of Bone Marrow Mesenchymal Stem Cells Derived Exosomes on Proliferation and Differentiation of Osteoblasts

CHEN Yan*, JIANG Sheng-jun, PENG You-jian   

  1. Department of Stomatology, Renmin Hospital of Wuhan University, Wuhan 430060,China
  • Received:2018-08-31 Online:2019-04-28 Published:2019-04-23

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摘要： 目的: 探讨骨髓间充质干细胞来源的外泌体对成骨细胞增殖和分化的影响。方法: 体外分离培养骨髓间充质干细胞,超速离心法收集外泌体,并对成骨细胞进行刺激,细胞计数试剂盒(cell counting kit-8,CCK-8)法检测骨髓间充质干细胞来源的外泌体对成骨细胞增殖的影响,碱性磷酸酶活性检测及茜素红染色观察骨髓间充质干细胞来源的外泌体对成骨细胞矿化的影响,利用实时荧光定量聚合酶链反应(real-time polymerase chain reaction,Real-time PCR)检测成骨相关基因的表达。结果: 骨髓间充质干细胞来源的外泌体促进成骨细胞的增殖,上调成骨相关基因的表达,提升碱性磷酸酶活性及促进钙化结节的形成。结论: 骨髓间充质干细胞来源的外泌体能够促进成骨细胞的增殖和分化。

关键词: 骨髓间充质干细胞, 外泌体, 成骨细胞, 增殖, 分化

Abstract: Objective: To evaluate the effects of bone marrow mesenchymal stem cells (BMSCs) derived exosomes on the proliferation and differentiation of osteoblasts. Methods: BMSCs were cultured in vitro and exsomes were isolated by ultracentrifugation from supernatants. After treated with BMSCs derived exosomes, the proliferation of osteoblasts was detected by CCK-8 assay. Alkaline phosphatase activity (ALP) analysis and alizarin red staining were adopted to evaluate the effect of BMSCs derived exosomes on mineralization process of osteoblasts. Real-time PCR was utilized to detect the expression of odontogenesis-related genes. Results: BMSCs derived exosomes enhanced the proliferation of osteoblasts and upregulated the expression levels of odontogenesis-related genes. The ALP activity and calcium accumulation of osteoblasts were increased significantly as well. Conclusion: BMSCs derived exosomes could promote the proliferation and differentiation of osteoblasts.

Key words: Bone marrow mesenchymal stem cells, Exosome, Osteoblast, Proliferation, Differentiation