口腔医学研究

口腔医学研究 ›› 2017, Vol. 33 ›› Issue (7): 693-697.DOI: 10.13701/j.cnki.kqyxyj.2017.07.002

• 基础研究论著 • 上一篇    下一篇

钛表面RGD/仿生磷酸钙复合涂层对MC3T3-E1细胞黏附、增殖、分化的影响

曹亚洲1, 程伟2, 胡勤刚1*   

  1. 1. 南京大学医学院附属口腔医院口腔颌面外科 江苏 南京 210008;
    2. 南京大学医学院附属口腔医院种植科 江苏 南京 210008
  • 收稿日期:2016-12-30 出版日期:2017-07-20 发布日期:2017-07-27
  • 通讯作者: 胡勤刚,E-mail: qghu@nju.edu.cn
  • 作者简介:曹亚洲(1990~),男,山东人,硕士,主要从事钛种植体表面改性的研究工作。
  • 基金资助:
    国家自然科学基金(编号:81402238,81271698)

Effect of RGD/biomimetic Calcium Phosphate Coatings on Adhesion, Proliferation and Differentiation of MC3T3-E1 Cells on Titanium.

CAO Ya-zhou1, CHENG Wei2, HU Qin-gang1*.   

  1. 1. Department of Oral and Maxillofacial Surgery, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing 210008, China;
    2. Department of Dental Implantation, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing 210008, China.
  • Received:2016-12-30 Online:2017-07-20 Published:2017-07-27

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被引次数

1

摘要： 目的:研究钛表面RGD/仿生磷酸钙复合涂层对MC3T3-E1细胞黏附、增殖和分化的影响。方法:1)两步法在钛表面制备仿生磷酸钙涂层,扫描电子显微镜(SEM)观察形态,X射线衍射(XRD)、傅立叶转换红外光谱(FTIR)检测表征;2)RGD固定至仿生磷酸钙涂层;3)实验分3组:纯钛组,钛/仿生磷酸钙涂层组,钛/RGD/仿生磷酸钙复合涂层组,细胞分别接种至3组材料表面。4)SEM观察细胞接种4 h和24 h的形态;5)结晶紫染色检测细胞接种4 h和8 h的黏附情况。6)MTT法检测细胞1、3、7 d的增殖活性;7)检测细胞3、7、14 d的ALP活性。结果:SEM细胞形态观察及结晶紫染色结果显示,RGD/仿生磷酸钙复合涂层组的细胞黏附效果优于对照组。MTT结果显示RGD/仿生磷酸钙复合涂层组的细胞增殖活性强于对照组。ALP检测结果显示RGD/仿生磷酸钙复合涂层组的成骨分化活性高于对照组。结论:钛表面RGD/仿生磷酸钙复合涂层对MC3T3-E1细胞的黏附、增殖、分化均有促进作用。

关键词: RGD, 仿生磷酸钙涂层, MC3T3-E1细胞, 黏附, 增殖, 分化

Abstract: Objective: To evaluate the effect of biomimetic calcium phosphate coatings containing RGD on adhesion, proliferation and differentiation of MC3T3-E1 cells. Methods: A 2-step approach was adopted to prepare biomimetic calcium phosphate coatings on the surface of titanium. The characterization of the coatings was analyzed by scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy, and X-ray diffractionometry. The RGD was incorporated into coatings through co-precipitation. MC3T3-E1 cells were seeded on Ti, Ti/ biomimetic calcium phosphate coatings, and Ti/RGD/biomimetic calcium phosphate coatings, respectively. The morphology of MC3T3-E1 cells cultured on samples was observed by SEM. The attachment of MC3T3-E1 cells on various samples was determined by crystal violet staining assay. The proliferation of MC3T3-E1 cells was determined by MTT after cultured for 1, 3 and 7 days. The ALP activity of the MC3T3-E1 cells was measured after cells cultured on various samples for 3, 7 and 14 days. Results: The results of SEM and crystal violet assay showed that the adhesion of cells growing on RGD/biomimetic calcium phosphate coatings was greater than that of control groups. Moreover, MTT test showed that the proliferation of cells growing on RGD/biomimetic calcium phosphate coatings was greater compared with that in control groups. In addition, the ALP activity of MC3T3-E1 cells cultured on RGD/biomimetic calcium phosphate coatings was higher than that in control groups. Conclusion: RGD/biomimetic calcium phosphate coatings can promote adhesion, proliferation, and differentiation of MC3T3-E1 cells.

Key words: RGD , Biomimetic calcium phosphate coatings, MC3T3-E1 cells , Adhesion, Proliferation , Differentiation

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