口腔医学研究

口腔医学研究 ›› 2019, Vol. 35 ›› Issue (2): 142-146.DOI: 10.13701/j.cnki.kqyxyj.2019.02.010

• 口腔颌面外科学研究 • 上一篇    下一篇

人源性长寿保障基因2在口腔鳞癌组织中表达及对细胞增殖和侵袭能力的影响

汪晓龙1,曹顺顺1,张鹏1,舒传继1,夏林2*   

  1. 1. 鄂东医疗集团市中心医院,湖北理工学院附属医院口腔科 湖北 黄石 435000;
    2. 鄂东医疗集团市中心医院,湖北理工学院附属医院头颈肿瘤内科 湖北 黄石 435000
  • 收稿日期:2018-08-21 出版日期:2019-02-18 发布日期:2019-02-25
  • 通讯作者: 夏林,E-mail:33680406@qq.com
  • 作者简介:汪晓龙(1977~ ),男,湖北鄂州人,主治医师,硕士,主要从事口腔临床和科研工作。

Expression of Homo Sapiens Longevity Assurance Homologue 2 of Yeast LAG1 in Oral Squamous Cell Carcinoma and Its Effect on Cell Proliferation and Invasion.

WANG Xiao-long1, CAO Shun-shun1, ZHANG Peng1, SHU Chuan-ji1, XIA Lin2*   

  1. 1. Department of Stomatology, Edong Medical Group Huangshi Central Hospital, Affiliated Hospital of Hubei Institute of Technology, Huangshi 435000, China;
    2. Department of Head and neck oncology, Edong Medical Group Huangshi Central Hospital, Affiliated Hospital of Hubei Institute of Technology, Huangshi 435000, China.
  • Received:2018-08-21 Online:2019-02-18 Published:2019-02-25

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摘要： 目的: 探讨人源性长寿保障基因2(LASS2)在口腔鳞癌组织中表达,以及沉默人口腔鳞癌CAL-27细胞中LASS2基因表达对细胞增殖和侵袭能力的影响。方法: 选取口腔鳞癌患者64例,利用实时荧光定量PCR技术和Western blot法检测口腔鳞癌和癌旁组织中LASS2基因和蛋白表达,培养CAL-27细胞并分为siRNA-LASS2组、阴性对照组和空白组,实时荧光定量PCR技术检测LASS2基因表达,MTT法检测细胞增殖,划痕实验检测细胞迁移能力,Transwell小室法检测细胞侵袭力。结果: 口腔鳞癌组织中LASS2 mRNA和蛋白相对表达量均低于癌旁组织(P<0.05);口腔鳞癌组织中LASS2 mRNA和蛋白相对表达量与TNM分期、分化程度、临床分期和淋巴结转移有关(P<0.05);siRNA-LASS2组细胞中LASS2 mRNA相对表达量低于阴性对照组和空白组(P<0.05);siRNA-LASS2组1、2、3和4 d时A值均高于阴性对照组和空白组(P<0.05);siRNA-LASS2组24 h和48 h时细胞迁移率高于阴性对照组和空白组(P<0.05);siRNA-LASS2组侵袭细胞数高于阴性对照组和空白组(P<0.05)。结论: LASS2在口腔鳞癌组织中呈低表达,特异性抑制口腔鳞癌细胞中LASS2表达可促进细胞增殖、加速细胞迁移及侵袭。

关键词: 口腔鳞癌, 人源性长寿保障基因2, 细胞增殖, 侵袭力

Abstract: Objective : To investigate the expression of homo sapiens longevity assurance homologue 2 of yeast LAG1 (LASS2) in oral squamous cell carcinoma, and explore the effect of silencing LASS2 gene expression on proliferation and invasion of CAL27 cells. Methods: A total of 64 cases of patients with oral squamous cell carcinoma were selected. The expressions of LASS2 gene and protein in oral squamous cell carcinoma and adjacent tissues were detected by using real-time quantitative PCR and Western blot. CAL-27 cells were cultured and divided into siRNA-LASS2 group, negative control group, and blank group. The expression of LASS2 gene in cells was detected by using real-time quantitative PCR. The cell proliferation was detected by using MTT assay. The cell migration was detected by using scratch test. The cell invasive was detected by using Transwell chamber. Results: The relative expression levels of LASS2 mRNA and protein in the oral squamous cell carcinoma tissues were lower than those in the adjacent tissues (P<0.05). The relative expression levels of LASS2 mRNA and protein in oral squamous cell carcinoma tissues were related to TNM stage, differentiation degree, clinical stage, and lymph node metastasis (P<0.05). The relative expression level of LASS2 mRNA in the siRNA-LASS2 group was lower than those in the negative control group and the blank group (P<0.05). The absorbance A values at 1d, 2d, 3d, and 4d in the siRNA-LASS2 group were higher than those in the negative control group and the blank group (P<0.05). The cell migration rates at 24h and 48h in the siRNA-LASS2 group were higher than those in the negative control group and the blank group (P<0.05). The number of invasive cells in the siRNA-LASS2 group was higher than that in the negative control group and the blank group (P<0.05). Conclusion: LASS2 was lowly expressed in oral squamous cell carcinoma tissues. Specific inhibition of LASS2 expression in oral squamous cell carcinoma cells could promote cell proliferation and accelerate cell migration and invasion.

Key words: Oral squamous cell carcinoma, Homo sapiens longevity assurance homologue 2 of yeast LAG1 Cell proliferation, Invasiveness