箭根酮对LPS刺激牙周膜成纤维细胞增殖能力的影响

doi:10.13701/j.cnki.kqyxyj.2018.06.019

口腔医学研究 ›› 2018, Vol. 34 ›› Issue (6): 653-656.DOI: 10.13701/j.cnki.kqyxyj.2018.06.019

箭根酮对LPS刺激牙周膜成纤维细胞增殖能力的影响

周茜雯¹, 曾豪¹, 张嘉昕¹, 刘恭奇², 王飒², 汪烈^1*

1. 武汉科技大学附属普仁医院口腔科湖北武汉 430081;
2. 武汉大学口腔医院湖北武汉 430079

收稿日期:2018-01-29 出版日期:2018-06-20 发布日期:2018-06-21
通讯作者: 汪烈,E-mail:2503723909@qq.com
作者简介:周茜雯(1975~ ),女,湖北武汉人,学士,主治医师,主要从事口腔科临床治疗工作。

Effects of Taccaloniolde on LPS - stimulated Proliferation of Periodontal Ligament Fibroblasts

ZHOU Qian-wen¹, ZENG Hao¹, ZHANG Jia-xin¹, LIU Gong-qi², WANG Sha², WANG Lie^1*

1. Department of Stomatology, Pu Ren Hospital Affiliated to Wuhan University of Science and Technology, Wuhan 430081, China;
2. Hospital of Stomatology, Wuhan University, Wuhan 430079, China

Received:2018-01-29 Online:2018-06-20 Published:2018-06-21

摘要/Abstract

摘要： 目的:研究箭根酮对LPS刺激牙周膜成纤维细胞增殖能力的影响及机制研究。方法:将人牙周膜成纤维细胞培养在含LPS的培养液中,同时加入或不加不同浓度的箭根酮,同时设不含箭根酮的空白对照组,24、48、72 h 后,以CCK-8 法检测细胞的增殖情况。流式检测细胞凋亡情况。结果:加药培养24、48、72 h后,不同浓度箭根酮组细胞的相对活性明显低于对照组和LPS刺激组,结果有统计学意义(P<0.001)。流式结果表明,相比于对照组,箭根酮能够显著促进细胞凋亡。结论:箭根酮对LPS刺激的牙周膜成纤维细胞增殖具有明显抑制作用,且其可能机制为促进细胞凋亡。

关键词: 箭根酮, 成纤维细胞, 细胞增殖, 细胞凋亡

Abstract: Objective: To research the effect and mechanism of taccaloniolde on LPS-stimulated proliferation of human periodontal ligament fibroblasts. Methods: Human periodontal ligament fibroblasts were cultured in LPS-containing medium with or without different concentrations of taccaloniolde. At the same time, the blank control group without taccaloniolde was established. After 24, 48, and 72 hours, the cell proliferation was detected by CCK-8 assay and the cell apoptosis was detected by flow cytometry. Results: After 24, 48, and 72 h of drug addition, the relative cell viability in the different doses of taccaloniolde was significantly lower than that stimulated with the control and LPS. Compared with the control group, taccaloniolde could promote apoptosis significantly. Conclusion: Taccaloniolde can significantly inhibited cell proliferation of human periodontal ligament fibroblasts increased by LPS-stimulated, and its possible mechanism is due to promote apoptosis.

Key words: Taccaloniolde, Fibroblasts, Cell proliferation, Apoptosis

周茜雯, 曾豪, 张嘉昕, 刘恭奇, 王飒, 汪烈. 箭根酮对LPS刺激牙周膜成纤维细胞增殖能力的影响[J]. 口腔医学研究, 2018, 34(6): 653-656.

ZHOU Qian-wen, ZENG Hao, ZHANG Jia-xin, LIU Gong-qi, WANG Sha, WANG Lie. Effects of Taccaloniolde on LPS - stimulated Proliferation of Periodontal Ligament Fibroblasts[J]. Journal of Oral Science Research, 2018, 34(6): 653-656.

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箭根酮对LPS刺激牙周膜成纤维细胞增殖能力的影响

Effects of Taccaloniolde on LPS - stimulated Proliferation of Periodontal Ligament Fibroblasts

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