TNF-α对牙周膜成纤维细胞ephrinB2/EphB4表达量的影响

doi:10.13701/j.cnki.kqyxyj.2020.08.009

口腔医学研究 ›› 2020, Vol. 36 ›› Issue (8): 741-744.DOI: 10.13701/j.cnki.kqyxyj.2020.08.009

TNF-α对牙周膜成纤维细胞ephrinB2/EphB4表达量的影响

王琳璇¹, 王琦¹, 赵云¹, 刘一鸣¹, 韩梅^1,2, 米方林^1,2*

1. 川北医学院口腔医学系四川南充 637000;
2. 川北医学院附属医院口腔科四川南充 637000

收稿日期:2019-11-21 出版日期:2020-08-28 发布日期:2020-08-18
通讯作者: *米方林,E-mail:mfl126@126.com
作者简介:王琳璇(1992~ ),女,陕西韩城人,硕士,研究方向:牙体牙髓病学。
基金资助:
四川省教育厅科研项目(编号:15ZA0210);南充市研发资金项目(编号:16YFZJ0123);南充市市校科技战略合作专项(编号:NSMC20170442)

Effect of TNF-α on Expression of ephrinB2/EphB4 in Periodontal Fibroblasts

WANG Linxuan¹, WANG Qi¹, ZHAO Yun¹, LIU Yiming¹, HAN Mei^1,2, MI Fanglin^1,2*

1. Department of Stomatology, Chuanbei Medical College, Nanchong 637000, China;
2. Department of Stomatology, Affiliated Hospital of Chuanbei Medical College, Nanchong 637000, China

Received:2019-11-21 Online:2020-08-28 Published:2020-08-18

摘要/Abstract

摘要： 目的: 检测不同浓度TNF-α对牙周膜成纤维细胞ephrinB2/EphB4表达量的影响。方法: 收集因正畸需要拔除的健康牙齿,培养原代牙周膜成纤维细胞;用不同浓度TNF-α分别刺激24 h,48 h后检测牙周膜细胞ephrinB2/EphB4 mRNA及蛋白表达量。结果: 24 h组ephrinB2与EphB4 mRNA浓度均降低,差异有统计学意义(P<0.05);48 h低浓度组对ephrinB2 mRNA表达量无影响,0.1 μg/L使EphB4 mRNA表达量增加,高浓度组使ephrinB2/EphB4 mRNA表达量下降;24 h组,TNF-α浓度为0.1~1 μg/L时,ephrinB2/EphB4蛋白表达量无明显变化,TNF-α浓度为10~1000 μg/L时ephrinB2/EphB4蛋白表达量下降;48 h组,0.1 μg/L TNF-α作用后使EphB4蛋白表达量上升,ephrinB2蛋白表达量无明显变化,1 μg/L作用后,ephrinB2/EphB4蛋白表达量均无明显变化, 10~1000 μg/L刺激后ephrinB2/ EphB4蛋白表达量均下降。结论: 24 h时TNF-α使ephrinB2/ EphB4 mRNA与蛋白表达量均降低,但在48 h时,低浓度作用下EphB4表达量上升,是否可以为低浓度炎症状态下骨改建的研究提供参考。

关键词: 肿瘤坏死因子-α, 牙周膜成纤维细胞, 促红细胞生成素肝细胞激酶受体膜结合配体B2, 促红细胞生成素肝细胞激酶受体B4, 骨改建, 牙周膜

Abstract: Objective: To investigate the effects of TNF-α on the expression of ephrinB2/EphB4 mRNA and protein levels in periodontal fibroblasts. Methods: Healthy teeth which need to be extracted due to orthodontic treatment were collected and the primary periodontal fibroblasts were cultured. The expressions of ephrinB2/EphB4 mRNA and protein levels were detected after the periodontal ligament fibroblasts were stimulated with different concentrations of TNF-α after 24 h and 48 h. Results: The mRNA concentrations of ephrinB2 and EphB4 were both decreased after 24 h (P<0.05). After 48 h, the low concentration group (0.1 μg/L-1 μg/L) had no effect on ephrinB2 expression. 0.1 μg/L could increase the expression of EphB4. However, the high concentration could decrease the expression of ephrinB2/ EphB4. Conclusion: ephrinB2/EphB4 was decreased by TNF-α after 24 h, however, EphB4 expression was increased after 48 h, which was implied to be a reference to study the bone remodeling in low-concentration inflammatory state.

Key words: tumor necrosis factor alpha, periodontal ligament fibroblasts, ephrinB2, EPHB4, tooth movement, periodontitis

王琳璇, 王琦, 赵云, 刘一鸣, 韩梅, 米方林. TNF-α对牙周膜成纤维细胞ephrinB2/EphB4表达量的影响[J]. 口腔医学研究, 2020, 36(8): 741-744.

WANG Linxuan, WANG Qi, ZHAO Yun, LIU Yiming, HAN Mei, MI Fanglin. Effect of TNF-α on Expression of ephrinB2/EphB4 in Periodontal Fibroblasts[J]. Journal of Oral Science Research, 2020, 36(8): 741-744.

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TNF-α对牙周膜成纤维细胞ephrinB2/EphB4表达量的影响

Effect of TNF-α on Expression of ephrinB2/EphB4 in Periodontal Fibroblasts

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