口腔医学研究

口腔医学研究 ›› 2018, Vol. 34 ›› Issue (11): 1208-1211.DOI: 10.13701/j.cnki.kqyxyj.2018.11.015

• 口腔肿瘤学研究 • 上一篇    下一篇

华卟啉钠介导的光动力疗法对人舌鳞癌细胞株HSC-4的增殖和迁移能力的影响

黄宇思1, 张磊1*, 刘乐天2, 马垚1, 刘鑫1   

  1. 1. 哈尔滨医科大学口腔医学院口腔预防保健科 黑龙江 哈尔滨 150001;
    2. 哈尔滨医科大学口腔医学院口腔颌面外科 黑龙江 哈尔滨 150001
  • 收稿日期:2018-06-06 出版日期:2018-11-28 发布日期:2018-11-23
  • 通讯作者: 张磊,E-mail:13845083021@163.com
  • 作者简介:黄宇思(1992~ ),女,黑龙江鹤岗人,硕士在读,主要从事口腔内科的临床工作。
  • 基金资助:
    哈尔滨医科大学附属第一医院科研基金(编号:2014B19)

Effects of DVDMS-PDT on Proliferation and Migration of Human Tongue Squamous Carcinoma Cell Line HSC-4

HUANG Yu-si1, ZHANG Lei1*, LIU Le-tian2, MA Yao1, LIU Xin1   

  1. 1. Department of Oral Health Sciences, The Affiliated Stomatological Hospital of Harbin Medical University, Harbin 150001, China;
    2. Department of Oral and Maxillofacial Surgery, The Affiliated Stomatological Hospital of Harbin Medical University, Harbin 150001, China.
  • Received:2018-06-06 Online:2018-11-28 Published:2018-11-23

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摘要： 目的:探讨华卟啉钠(sinoporphyrin sodium,DVDMS)介导的光动力疗法(photodynamic therapy,PDT)对体外培养的口腔舌鳞状细胞癌(oral tongue squamous cell carcinoma,OTSCC,简称鳞癌)细胞株HSC-4的增殖及迁移能力的影响。方法:检测DVDMS细胞毒性,细胞摄取药物情况,将细胞分为对照组以及不同浓度的DVDMS-PDT处理组(DVDMS孵育浓度分别为0.1、0.5、1.0、2.0、3.0、4.0、5.0、6.0、8.0 mg/L),激光能量密度为0.75、1.50、3.00、6.00 J/cm2,细胞增殖检测试剂盒(cell counting kit-8,CCK-8)法检测DVDMS-PDT对细胞增殖能力的影响,划痕实验和Transwell小室实验观察细胞迁移能力的变化。结果:DVDMS毒性实验显示单纯药物对细胞毒性较小,联合激光照射后,可显著抑制HSC-4细胞增殖(P均<0.05),与对照组比较,划痕愈合率降低,Transwell小室穿膜细胞数减少(P均<0.05)。DVDMS药物浓度2.0 mg/L,体外孵育6 h,光能量密度在3.00 J/cm2以上可有效杀伤HSC-4。结论:DVDMS-PDT可有效杀伤舌鳞癌细胞,抑制HSC-4的迁移。光敏剂浓度、孵育时间、光能量密度是影响疗效的重要因素。

关键词: 华卟啉钠, 光动力疗法, 舌鳞癌, 迁移

Abstract: Objective: To study the proliferation and migration of oral tongue squamous cell carcinoma HSC-4 cells induced by DVDMS-PDT. Methods: To detect the cytotoxicity of DVDMS and the uptake of drugs by cells, the cells were divided into control group and DVDMS-PDT treatment group with different concentrations (0.1, 0.5, 1.0, 2.0, 3.0, 4.0, 5.0, 6.0, and 8.0 mg/L, respectively). The laser energy density were 0.75, 1.50, 3.00, 6.00J/cm2. The effects of different drug concentrations and light energy density on cell viability were analyzed by CCK-8. Scratch assay and Transwell experiment were used to observe the changes of cell migration ability. Results: DVDMS toxicity experiments showed that the single drug had less cytotoxicity, and combined with laser irradiation, it could significantly inhibit the proliferation of HSC-4 cells (P<0.05). Compared with the control group, the healing rate of scratch assay was decreased,and the number of transwell penetrated cells was decreased (P<0.05). DVDMS concentration at 2.0 mg/L, 6 hours incubation, and light energy density above 3.00J/cm2 could effectively kill HSC-4 cells. Conclusion: DVDMS-PDT can effectively kill the tongue squamous cell carcinoma cell line and inhibit the migration of HSC-4 cells. Photosensitizer concentration, incubation time, and light energy density are important factors affecting the therapeutic effect.

Key words: Sinoporphyrin sodium, Photodynamic therapy, Tongue squamous cell carcinoma, Migration