口腔医学研究

口腔医学研究 ›› 2019, Vol. 35 ›› Issue (6): 559-562.DOI: 10.13701/j.cnki.kqyxyj.2019.06.012

• 口腔肿瘤学研究 • 上一篇    下一篇

慢病毒介导的Med19表达下调提高舌鳞癌细胞顺铂敏感性

王蕊,李立恒,胥爱文,安峰*   

  1. 河北北方学院附属第一医院口腔科 河北 张家口 075000
  • 收稿日期:2018-10-23 出版日期:2019-06-28 发布日期:2019-06-27
  • 通讯作者: 安峰,E-mail:fhwei9@163.com
  • 作者简介:王蕊(1982~ ),女,河北廊坊人,本科,主治医师,主要从事口腔科临床治疗工作。
  • 基金资助:
    河北省科学技术厅重点研发计划自筹项目(编号:172777144)

Lentivirus Mediated Down-regulation of Med19 Expression Enhances Sensitivity of Tongue Squamous Cell Carcinoma to Cisplatin

WANG Rui, LI Li-heng, XU Ai-wen, AN Feng*   

  1. Department of Stomatology, The First Affiliated Hospital of Hebei North University, Zhangjiakou 075000, China.
  • Received:2018-10-23 Online:2019-06-28 Published:2019-06-27

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摘要： 目的:探讨慢病毒介导的中介体19(Med19)表达下调对舌鳞癌细胞顺铂敏感性的影响。方法:qRT-PCR和Western blot检测舌鳞癌细胞和正常口腔上皮细胞中Med19表达变化。在舌鳞癌细胞中转染Med19 shRNA,以顺铂处理下调Med19的舌鳞癌细胞,MTT和流式细胞术测定增殖和凋亡,Western blot检测活化型Caspase-3(C-Caspase-3)、活化型Caspase-9(C-Caspase-9)、Bcl-2相关X蛋白( Bax)蛋白水平,Western blot检测胞浆和线粒体中细胞色素C(Cyt-c)蛋白水平。结果:Med19在舌鳞癌细胞中表达水平升高。Med19 shRNA转染可以下调舌鳞癌细胞中Med19的表达水平。下调Med19或顺铂处理后的舌鳞癌细胞增殖能力均降低,细胞凋亡率升高,细胞中C-Caspase-3、C-Caspase-9、Bax蛋白水平升高,胞浆中Cyt-c蛋白表达水平升高,线粒体中Cyt-c蛋白表达水平降低。下调Med19联合顺铂处理对舌鳞癌细胞增殖、凋亡及C-Caspase-3、C-Caspase-9、Bax和线粒体释放Cyt-c影响作用更大。结论:下调Med19能够提高舌鳞癌细胞顺铂敏感性。

关键词: Med19, 舌鳞癌, 顺铂, 凋亡

Abstract: Objective: To investigate the effect of down-regulation of lentivirus-mediated mediator 19 (Med19) expression on the sensitivity of tongue squamous cell carcinoma cells to cisplatin. Methods: Med19 shRNA was transfected into tongue squamous cell carcinoma cells. The interference effects were measured by qRT-PCR and Western blot. Cisplatin treated tongue squamous cell carcinoma with Med19 downregulation. MTT and flow cytometry were used to detect the cell proliferation and apoptosis. The levels of C-Caspase-3, C-Caspase-9, and Bax were detected by Western blotting, and the levels of Cyt-c in cytoplasm and mitochondria were detected by Western blotting. Results: The expression level of Med19 in tongue squamous cell carcinoma increased. Transfection of Med19 shRNA could reduce the expression level of Med19 in tongue squamous cell carcinoma. The proliferation of tongue squamous cell carcinoma treated with Med19 or cisplatin decreased, and the rate of apoptosis increased, the level of C-Caspase-3, C-Caspase-9 and Bax protein increased in cells. The expression level of Cyt-c protein in the cytoplasm increased, and the expression level of Cyt-c protein in mitochondria decreased. The effect of down-regulation of Med19 combined with cisplatin on the proliferation and apoptosis of tongue squamous cell carcinoma cells and the release of Cyt-c from C-Caspase-3, C-Caspase-9, Bax and mitochondria were greater. Conclusion: Down regulation of Med19 can enhance the sensitivity of tongue squamous cell carcinoma to cisplatin.

Key words: Med19, Tongue squamous cell carcinoma, Cisplatin, Apoptosis