口腔医学研究

口腔医学研究 ›› 2019, Vol. 35 ›› Issue (10): 979-983.DOI: 10.13701/j.cnki.kqyxyj.2019.10.017

• 口腔肿瘤学研究 • 上一篇    下一篇

Beclin1与凋亡相关蛋白在口腔鳞癌中的表达及意义

孙维克, 王培源, 刘伟, 王霞*   

  1. 滨州医学院 山东 烟台 264003
  • 收稿日期:2019-06-09 出版日期:2019-10-28 发布日期:2019-10-22
  • 通讯作者: 王霞,E-mail:wangxia7512@163.com
  • 作者简介:孙维克(1979~ ),女,山东人,讲师,硕士,研究方向:口腔癌、口腔黏膜病。

Expression and Significance of Beclin1 and Apoptosis Related Proteins in Oral Carcinogenesis

SUN Weike, WANG Peiyuan, LIU Wei, WANG Xia*   

  1. Binzhou Medical University, Yantai 264000, China
  • Received:2019-06-09 Online:2019-10-28 Published:2019-10-22

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摘要： 目的:探讨Beclin1及凋亡相关蛋白bcl-2、survivin及bax在口腔鳞癌中的表达及临床意义。方法:采用免疫组化SP法检测口腔黏膜癌变组织中(包括鳞癌60例,异常增生48例,癌旁正常口腔黏膜60例)Beclin1、bcl-2、survivin及bax的表达,分析其相关性及临床意义。结果:Beclin1在鳞癌和正常黏膜呈高水平表达(表达阳性率分别为65%,75%),在异常增生组的表达显著性降低(54.2%,P＜0.05);bcl-2及survivin在鳞癌组织中表达水平(70%,73.33%)显著高于异常增生和正常黏膜(P<0.05),bax则在异常增生组表达显著高于鳞癌和正常黏膜(P<0.05)。口腔癌组织Beclin1的表达与bcl-2、survivin、bax的表达均密切相关(P＜0.05;相关系数分别为r=0.421,r=0.367,r=0.542),四者均与肿瘤大小及颈部淋巴结转移密切相关。结论:Beclin1与凋亡相关蛋白在口腔鳞癌的癌变过程中密切相关,自噬与凋亡可能协同参与口腔癌的进展。

关键词: Beclin1, 自噬, 凋亡, 口腔癌

Abstract: Objective: To study the clinical significance of Beclin1 and its relationship with bcl-2, survivin, and bax in oral carcinogenesis. Methods: Immunohistochemical SP method was used to detect the expression of Beclin1, bcl-2, survivin, and bax in oral carcinogenesis tissues including oral cancer (60 cases), oral dysplasia (48 cases), and normal oral mucosa (60 cases), and to study the clinical significance of Beclin 1 in oral squamous cell carcinoma (OSCC) and its relationship with bcl-2, survivin, and bax expression in oral carcinogenesis. Results: The expression of Beclin1 was significantly increased in OSCC and normal mucosa (65% and 75%, respectively), and the positive expression rate in dysplasia significantly decreased (54.2%, P<0.05). Survivin and bcl-2 expression were higher in OSCC (73.33% and 70%) than in dysplasia and normal mucosa (P<0.05); the expression rate of bax increased in dysplasia than OSCC and normal mucosa (P<0.05). Beclin1, bcl-2, survivin, and bax expression was closely related to diameter of tumor and lymph node metastasis (P<0.05). The expression of Beclin1 was closely related to bcl-2, survivin, and bax (P<0.05; r=0.421, 0.367 and 0.542) in OSCC. Conclusion: Autophagy and apoptosis were involved in oral carcinogenesis, and were associated with invasion and metastasis of oral cancer, which synergistically participated in the development of oral cancer

Key words: Beclin1, autophagy, apoptosis, oral carcinoma