Eya4在口腔鳞状细胞癌中的表达及其意义

doi:10.13701/j.cnki.kqyxyj.2019.06.011

口腔医学研究 ›› 2019, Vol. 35 ›› Issue (6): 555-558.DOI: 10.13701/j.cnki.kqyxyj.2019.06.011

Eya4在口腔鳞状细胞癌中的表达及其意义

高旭,周阳,李琛^*,薛晓寒,魏秀峰^*

吉林大学口腔医学院口腔黏膜科,吉林省牙发育及颌骨重塑与再生重点实验室吉林长春 130021

收稿日期:2018-11-01 出版日期:2019-06-28 发布日期:2019-06-27
通讯作者: 李琛,E-mail:lichen@jlu.edu.cn
魏秀峰,E-mail:xfw79@sina.com
作者简介:高旭(1990~ ),男,山东青岛人,硕士在读,主要从事口腔黏膜病基础与临床方面的研究。
基金资助:
国家自然基金青年科学基金(编号:81500820)
吉林省科技厅资助课题(编号:20160101052JC)
吉林大学白求恩医学部青年基金(编号:2015431)

Expression and Significance of Eya4 in Oral Squamous Cell Carcinoma

GAO Xu, ZHOU Yang, LI Chen^*, XUE Xiao-han, WEI Xiu-feng^*

Department of Oral Mucosal Diseases, School of Stomatology, Jilin University, Key Laboratory of Dental Development and Jaw Remodeling and Regeneration in Jilin Province, Changchun 130021, China.

Received:2018-11-01 Online:2019-06-28 Published:2019-06-27

摘要/Abstract

摘要： 目的:研究eya4蛋白在口腔鳞状细胞癌(OSCC)中的表达及其意义。方法:免疫组织化学染色的方法检测eya4在60例OSCC患者的口腔黏膜组织和20例正常口腔黏膜组织(NOM)中的表达情况。结果:eya4在NOM组织以及高分化的OSCC组织中高表达,表达率分别为100%及95%,而在低分化的OSCC组织中表达率仅为5%,这表明eya4可能参与OSCC疾病发生发展的过程;此外通过研究eya4的表达与各项临床病理参数的关系显示,其表达情况与患者的年龄性别无关,而与肿瘤是否伴有淋巴结转移呈负相关即伴有淋巴结转移的病例中eya4表达水平明显低于不伴有淋巴结转移的患者。结论:eya4蛋白可能参与OSCC的疾病发生发展过程及转移过程。

关键词: EYA4, 口腔鳞状细胞癌, 免疫组织化学

Abstract: Objective: To study the expression and significance of eya4 protein in oral squamous cell carcinoma (OSCC). Methods: The expression of eya4 in oral mucosa of 60 patients with OSCC and 20 controls with normal oral mucosa (NOM) were detected by immunohistochemical staining. Results: Statistical analysis showed that eya4 was highly expressed in NOM tissues and highly differentiated OSCC squamous cell carcinoma tissues, with expression rates of 100% and 95%, respectively, while expression rate was only 5% in poorly differentiated OSCC squamous cell carcinoma tissues. This indicated that eya4 might be involved in the development of OSCC disease. In addition, by studying the relationship between the expression of eya4 and various clinicopathological parameters, the expression was not related to the age and sex of the patient. However, the eya4 expression levels were significantly lower in patients with lymph node metastasis than in patients without lymph node metastasis. Conclusion: Eya4 may influence the occurrence, development, and metastasis of OSCC.

Key words: EYA4, Oral squamous cell carcinoma, Immunohistochemistry

高旭,周阳,李琛,薛晓寒,魏秀峰. Eya4在口腔鳞状细胞癌中的表达及其意义[J]. 口腔医学研究, 2019, 35(6): 555-558.

GAO Xu, ZHOU Yang, LI Chen, XUE Xiao-han, WEI Xiu-feng. Expression and Significance of Eya4 in Oral Squamous Cell Carcinoma[J]. Journal of Oral Science Research, 2019, 35(6): 555-558.

参考文献

[1] Sun W, Gaykalova DA, Ochs MF,et al. Activation of the NOTCH pathway in head and neck cancer [J]. Cancer Res, 2014, 74(4):1091-1104.
[2] Takahashi H, Yanamoto S, Yamada S, et al. Effects of postoperative chemotherapy and radiotherapy on patients with squamous cell carcinoma of the oral cavity and multiple regional lymph node metastases [J]. Int J Oral Maxillofac Surg, 2014, 43(6):680-685.
[3] Nobuharu Y, Kazumichi S, Tomohiro Y, et al. A 5-year activity report from the oral cancer center, Tokyo dental college [J]. Bull Tokyo Dent Coll, 2013, 54(4): 265-273.
[4] 何黎明,郭新程,陶山松.CCND1、ORAOV1、ERCC1在舌鳞状细胞癌中的表达和临床意义[J].口腔医学研究,2015,31(6):597-600.
[5] 吕珊珊,魏秀峰,孙鑫.Pinx1在口腔鳞癌中的表达及意义[J].口腔医学研究,2016,32(10):1064-1066.
[6] 王贞超,杨开迆.P53基因的生物信息学分析[J].世界最新医学信息文摘,2017,17(2):34.
[7] Chu EC, Tamawski AS. PTEN regulatory functions in tumor suppression and cell biology [J]. Med Sci Monit, 2014, 10(10):235-241.
[8] Roye GD ,Myers RB, Brown D, et al. CD44 expression in dys-plastic epithelic and squamous-cell carcinoma of the esophagus [J]. Int J Cancer, 2006, 69:254-258.
[9] Clark SW, Fee BE, Cleveland JL. Misexpression of the eyes absent family triggers the apoptotic program [J]. J Biol Chem, 2002, 277 (5) :3560-3567.
[10] Xu PX, Adams J, Peters H, et al. Eya1- deficient mice lack ears and kidneys and show abnormal apoptosis of organ primordia [J]. Nat Genet, 1999, 23(1): 113-117.
[11] Miller SJ, Lan ZD, Hardiman A, et al. Inhibition of Eyes Absent Homolog 4 expression induces malignant peripheral nerve sheath tumor necrosis [J]. Oncogene, 2010, 29 (3): 368-379.
[12] Kim JJ, Grady WM. Epigenomic analysis of aberrantly methylated genes in colorectal cancer identifies genes commonly affected by epigenetic alterations [J]. Ann Surg Oncol, 2011, 18: 2338–2347.
[13] Osborn NK, Zou H, Molina JR, et al. Aberrant methylation of the eyes absent 4 gene in ulcerative colitis-associated dysplasia [J]. Clin Gastroenterol Hepatol, 2006, 4(2):212-218.
[14] Kim SJ, Tae CH, Hong SN, et al. EYA4 acts as a new tumor suppressor gene in colorectal cancer [J]. Mol Carcinog, 2015, 54(12):1748-1757.
[15] Hou X, Peng JX, Hao XY, et al.DNA methylation profiling identifies EYA4 gene as a prognostic molecular marker in hepatocellular carcinoma [J]. Ann Surg Oncol, 2014, 21(12):3891-3899.
[16] Hao XY, Cai JP, Liu X, et al. EYA4 gene functions as a prognostic marker and inhibits the growth of intrahepatic cholangiocarcinoma [J]. Chin J Cancer, 2016, 35(1):70.
[17] Zhang SK, Zheng RS, Chen Q, et al. Oral cancer incidence and mortalityin China,2011 [J]. Chin J Cancer Res, 2015, 27(1):44-51.
[18] Tadjuidje E, Hegde RS. The eyas absent proteins in development and disease [J]. Cell Mol Life Sci, 2013, 70(11):1897-1913.
[19] Towle R, Truong D, Garnis C. Epigenetic mediated silencing of EYA4 contributes to tumorigenesis in oral dysplastic cells [J]. Genes Chromosomes Cancer, 2016, 55(7):568-576.
[20] Zou H, Osborn NK, Harrington JJ, et al. Frequent methylation of eyas absent 4 gene in Barrett’s esophagus and esophageal adenocarcinoma [J]. Cancer Epidemiol Biomarkers Prev, 2005, 14(4):830-834.

Eya4在口腔鳞状细胞癌中的表达及其意义

Expression and Significance of Eya4 in Oral Squamous Cell Carcinoma

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

[1]	高安天, 林梓桐. 口腔鳞状细胞癌边界精准评价的光学成像研究进展[J]. 口腔医学研究, 2020, 36(5): 413-415.
[2]	杨凯成, 杨蕾, 赵建广, 罗风玉, 陈彦平, 崔子峰, 陈赫, 满莎莎. 口腔鳞状细胞癌患者外周血microRNA的表达及预后意义[J]. 口腔医学研究, 2020, 36(5): 428-432.
[3]	胡勤刚, 赵星星. 口腔鳞状细胞癌肿瘤相关成纤维细胞的研究进展[J]. 口腔医学研究, 2020, 36(4): 309-313.
[4]	郭俊峰, 张纲, 谭颖徽. CYP27B1在口腔鳞状细胞癌中的表达及其与预后的关系[J]. 口腔医学研究, 2020, 36(10): 930-933.
[5]	谢龙, 桑磊, 宋卓英, 江燕军. 唾液中微小RNA-375对口腔鳞状细胞癌的诊断价值及其对人口腔鳞状细胞癌细胞生物学功能的影响[J]. 口腔医学研究, 2019, 35(9): 883-888.
[6]	周童,张桐菲,张泽兵,全海英. 甲基硒酸对口腔癌细胞增殖、迁移、凋亡和周期的作用研究[J]. 口腔医学研究, 2019, 35(6): 568-572.
[7]	熊璟,何元春,刘杰,张英,李永强,邱勇棋,庄瑞. 白藜芦醇通过调控雷帕霉素靶蛋白/M2型丙酮酸激酶轴抑制口腔鳞状细胞癌细胞的糖酵解作用[J]. 口腔医学研究, 2019, 35(6): 573-576.
[8]	陆展翼, 胡勤刚, 陈盛, 杨艳, 丁亮, 泥艳红. 口腔鳞状细胞癌组织TGF-β1表达和临床病理学参数对预后的影响[J]. 口腔医学研究, 2019, 35(5): 433-438.
[9]	刘艳玲, 赵曦, 余丽, 聂敏海. SOX-9在口腔鳞状细胞癌组织中的表达及其临床意义[J]. 口腔医学研究, 2019, 35(5): 439-442.
[10]	白真玉, 王铠, 刘德裕, 黄谢山. 长链非编码RNA FAL1在口腔鳞状细胞癌中的表达及其与预后的关系[J]. 口腔医学研究, 2019, 35(4): 360-363.
[11]	王亚敏, 孟文霞, 周震, 郭薇, 谢宝仪. 口腔扁平苔藓组织中α-平滑肌肌动蛋白的表达及临床意义[J]. 口腔医学研究, 2019, 35(4): 393-396.
[12]	王晓乐, 贾荣. 口腔鳞状细胞癌患者年龄分布与肿瘤分子标记物的关系研究[J]. 口腔医学研究, 2019, 35(3): 238-241.
[13]	伍俊锋, 施斌, 曹裕杰, 陈法, 曹熔锴, 张诗蕾, 黄跃. 福建地区老年口腔鳞状细胞癌预后影响因素研究[J]. 口腔医学研究, 2019, 35(3): 242-245.
[14]	李勇伟,邱勋定,顾明,邓旎,谢莉莉. 同源形成素样蛋白2基因对口腔鳞状细胞癌侵袭、迁移及核因子-κB信号的影响[J]. 口腔医学研究, 2019, 35(2): 137-141.
[15]	孙志军, 武磊. 口腔鳞状细胞癌免疫检查点的研究进展[J]. 口腔医学研究, 2019, 35(12): 1107-1110.