人脐带间充质干细胞对舌鳞状细胞癌Cal-27细胞侵袭和迁移能力的影响

doi:10.13701/j.cnki.kqyxyj.2019.05.008

口腔医学研究 ›› 2019, Vol. 35 ›› Issue (5): 443-447.DOI: 10.13701/j.cnki.kqyxyj.2019.05.008

人脐带间充质干细胞对舌鳞状细胞癌Cal-27细胞侵袭和迁移能力的影响

张馨予^1#, 吴双燕^2#, 吴鸿¹, 徐竹青¹, 王云英³, 徐晓娜³, 李新¹, 郑建金^1*

1. 青岛大学口腔医学院山东青岛 266003;
2. 青岛市口腔医院多学科山东青岛 266001;
3. 青岛市市立医院中心实验室山东青岛 266000

收稿日期:2018-09-21 出版日期:2019-05-28 发布日期:2019-05-21
通讯作者: 郑建金,E-mail:zhjj19631016@sina.com
作者简介:张馨予(1991~ ),女,山东人,硕士在读,主要从事口腔颌面外科的研究学习。吴双燕(1965~ ),女,山东人,主任医师,硕导,主要从事牙体牙髓病临床、教学、科研工作。
基金资助:
青岛市科技局重点项目(编号:15-9-2-79-nsh)

Effect of Human Umbilical Cord Mesenchymal Stem Cells on Invasion and Migration of Cal-27 Cells from Tongue Squamous Cell Carcinoma.

ZHANG Xin-yu^1#, WU Shuang-yan^2#, WU Hong¹, XU Zhu-qing¹, WANG Yun-ying³, XU Xiao-na³, LI Xin¹, ZHENG Jian-jin^1*

1. School of Stomatology of Qingdao University, Qingdao 266003, China;
2. Qingdao Stomatological Hospital, Qingdao 266001, China;
3. Central Laboratory of Qingdao Municipal Hospital, Qingdao 266000, China.

Received:2018-09-21 Online:2019-05-28 Published:2019-05-21

摘要/Abstract

摘要： 目的: 探讨人脐带间充质干细胞(human umbilical cord blood mesenchymal stem cells,HUC-MSCs)对舌鳞状细胞癌Cal-27细胞侵袭和迁移能力的影响。方法: 细胞划痕实验检测HUC-MSCs分泌的细胞因子对Cal-27细胞迁移能力的影响;Transwell小室比较共培养前后Cal-27细胞的侵袭和迁移能力;逆转录-聚合酶链反应(reverse transcription-polymerase chain reaction,RT-PCR)检测共培养前后Cal-27细胞的侵袭、迁移相关基因的表达水平;Western blot技术检测肿瘤侵袭转移相关蛋白分子基质金属蛋白酶(matrix metalloproteinase,MMP)-2和MMP-9的蛋白表达的情况。结果: 条件培养基培养的Cal-27细胞的迁移距离短于对照组;共培养后Cal-27细胞的侵袭和迁移细胞数目减少;侵袭迁移相关基因、蛋白的表达水平下调。结论: HUC-MSCs与Cal-27细胞共培养可以抑制Cal-27细胞的侵袭和迁移。

关键词: 人脐带间充质干细胞, 舌鳞癌细胞Cal-27, 条件培养基, 侵袭和迁移, 基质金属蛋白酶

Abstract: Objective: To investigate the effect of human umbilical cord mesenchymal stem cells (HUC-MSCs) on the invasion and migration of Cal-27 cells in tongue squamous cell carcinoma. Methods: HUC-MSCs conditioned medium was prepared. Cal-27 cells were cultured and the effect of HUC-MSCs secreted factors on Cal-27 cell migration was detected by cell scratch test. The invasiveness and migration of Cal-27 cells before and after co-culture were compared in Transwell cells. Expression levels of genes related to invasion and migration of Cal-27 cells were detected by RT-PCR before and after co-culture. The changes of MMP2 and MMP9 protein expression in tumor invasion and metastasis-related protein molecules were detected by western blot. Results: The migration distance of Cal-27 cells was shorter than that of the control group. After co-culture, the number of invasion and migration of Cal-27 cells decreased. The expression levels of aggression-related genes and proteins were down-regulated. Conclusion: The co-culture of HUC-MSCs and Cal-27 cells can inhibit the invasion and migration of Cal-27 cells.

Key words: Human umbilical cord mesenchymal stem cells, Tongue squamous cell carcinoma Cal-27, Conditioned medium, Invasion and migration, Matrix metalloproteinases

张馨予, 吴双燕, 吴鸿, 徐竹青, 王云英, 徐晓娜, 李新, 郑建金. 人脐带间充质干细胞对舌鳞状细胞癌Cal-27细胞侵袭和迁移能力的影响[J]. 口腔医学研究, 2019, 35(5): 443-447.

ZHANG Xin-yu, WU Shuang-yan, WU Hong, XU Zhu-qing, WANG Yun-ying, XU Xiao-na, LI Xin, ZHENG Jian-jin. Effect of Human Umbilical Cord Mesenchymal Stem Cells on Invasion and Migration of Cal-27 Cells from Tongue Squamous Cell Carcinoma.[J]. Journal of Oral Science Research, 2019, 35(5): 443-447.

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人脐带间充质干细胞对舌鳞状细胞癌Cal-27细胞侵袭和迁移能力的影响

Effect of Human Umbilical Cord Mesenchymal Stem Cells on Invasion and Migration of Cal-27 Cells from Tongue Squamous Cell Carcinoma.

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