基质金属蛋白酶诱导因子和亲环素A在人根尖囊肿和根尖肉芽肿中的表达及临床意义

doi:10.13701/j.cnki.kqyxyj.2016.07.015

口腔医学研究 ›› 2016, Vol. 32 ›› Issue (7): 723-728.DOI: 10.13701/j.cnki.kqyxyj.2016.07.015

基质金属蛋白酶诱导因子和亲环素A在人根尖囊肿和根尖肉芽肿中的表达及临床意义

胡淑丽, 王艳青, 董艳, 张红艳^*

安徽医科大学口腔医学院,安徽医科大学附属口腔医院牙体牙髓科,安徽省口腔疾病研究中心实验室安徽合肥 230032

收稿日期:2015-12-09 出版日期:2016-07-26 发布日期:2016-07-25
通讯作者: 张红艳,E-mail:toothyan@126.com
作者简介:胡淑丽(1988~ ),女,安徽安庆人,硕士,主要从事牙体牙髓病学研究。
基金资助:
安徽省自然科学基金(编号:070413141);安徽高校省级自然科学研究项目(编号:KJ2011Z168);安徽省高校教学质量与教学改革工程项目(编号:20100352)

Expression and Clinical Significance of CD147 and CypA in Periapical Granulomas and Radicular Cysts.

HU Shu-li,WANG Yan-qing,DONG Yan,ZHANG Hong-yan.

Stomatologic Hospital & College, Anhui Medical University, Key Lab of Oral Diseases Research of Anhui Province, Hefei 230032,China

Received:2015-12-09 Online:2016-07-26 Published:2016-07-25

摘要/Abstract

摘要： 目的: 检测细胞外基质金属蛋白酶诱导因子(CD147)和亲环素A(CypA)在根尖肉芽肿和根尖囊肿中的表达,探讨CD147和CyPA在人慢性根尖周炎发生发展中的作用。方法: 收集经根尖手术切除所获得的根尖肉芽肿35例和根尖囊肿30例作为实验组,同时收集埋伏阻生智齿拔除术或行牙槽骨修整术凿下的8例健康牙槽骨作为正常对照组。CBCT图像记录病例的病损大小。运用免疫组织化学法检测所有样本中CD147和CypA的蛋白表达,分析CD147和CypA的蛋白表达水平。结果: 根尖囊肿组两种蛋白的表达水平均高于肉芽肿组(P<0.05)。CD147和CypA 的蛋白表达水平在根尖囊肿和根尖肉芽肿中呈正相关(P<0.05);CD147和CypA 的表达水平均与慢性根尖周炎的病变大小呈正相关(P<0.05)。结论: CD147和CypA可能参与根尖周病损的炎性反应和骨质吸收。CD147-CypA相互作用在人慢性根尖周病的发生发展过程中可能发挥了某种协同作用。

关键词: 细胞外基质金属蛋白酶诱导因子, 亲环素A, 根尖囊肿, 根尖肉芽肿

Abstract: Objective: To investigate the expression of extracellular matrix metalloproteinase inducer (CD147) and Cyclophilin A (CypA) in periapical granulomas (PGs) and radicular cysts (RCs). The interaction of CD147 with CypA and their roles in periapical pathogenesis were also speculated. Methods: Thirty PGs and thirty-five RCs were collected after periapical surgery as the experimental samples. Eight alveolar bone samples were obtained from extraction of impacted third molars or alveoloplasty as the control samples. The lesion size was recorded by CBCT. The protein expression of CD147 and CypA was observed by immunohistochemical analysis for all samples. Statistical analysis was performed to evaluate the differences of expression of CD147 and CypA. Results: The expression of CD147 and CypA in RCs were higher than in PGs (P<0.05). Positive correlations between CD147 and CypA protein levels were observed in PGs and RCs (P<0.05), respectively. Furthermore, the expression of CD147 and CypA was positively correlated with the lesion size (P<0.05). Conclusion: These findings demonstrated that CD147 and CypA might be involved in the inflammatory response, bone resorption of periapical lesions tissues. The CD147-CypA interactions may play a synergistic role in the development and progression of periapical lesion.

Key words: CD147, Cyclophilin A , Radicular cyst , Periapical granuloma

中图分类号:

R781.34

胡淑丽, 王艳青, 董艳, 张红艳. 基质金属蛋白酶诱导因子和亲环素A在人根尖囊肿和根尖肉芽肿中的表达及临床意义[J]. 口腔医学研究, 2016, 32(7): 723-728.

HU Shu-li,WANG Yan-qing,DONG Yan,ZHANG Hong-yan.. Expression and Clinical Significance of CD147 and CypA in Periapical Granulomas and Radicular Cysts.[J]. Journal of Oral Science Research, 2016, 32(7): 723-728.

参考文献

[1] Yang D,Wang J, Ni J, et al. Temporal expression of metalloproteinase-8 and-13 and their relationships with extracellular matrix metalloproteinase inducer in the development of ligature-induced periodontitis in rats [J]. Journal of periodontal research, 2013, 48(4)∶411-419
[2] Xiong L, Edwards C K, Zhou L. The biological function and clinical utilization of CD147 in human diseases: a review of the current scientific literature [J]. International journal of molecular sciences, 2014, 15(10)∶17411-17441
[3] Zhu X, Song Z, Zhang S, et al. CD147: A Novel Modulator of Inflammatory and Immune Disorders [J]. Current medicinal chemistry, 2014, 21(19)∶2138-2145
[4] Wang P,Heitman J. The cyclophilins [J]. Genome Biol, 2005, 6(2)∶226-230
[5] Sun S, Guo M, Zhang JB, et al. Cyclophilin A (CypA) interacts with NF-kappaB subunit,p65/RelA, and contributes to NF-kappaB activation signaling [J]. PLoS One, 2014, 9(8)∶e96211
[6] Obchoei S, Sawanyawisuth K, Wongkham C, et al. Secreted cyclophilin A mediates G1/S phase transition of cholangiocarcinoma cells via CD147/ERK1/2 pathway [J]. Tumor Biology, 2014∶1-11
[7] Seizer P, Gawaz M, May A E. Cyclophilin A and EMMPRIN (CD147) in cardiovascular diseases [J]. Cardiovascular research, 2014, 102(1)∶17-23
[8] Yuan W, Ge H, He B. Pro-inflammatory activities induced by CyPA–EMMPRIN interaction in monocytes [J]. Atherosclerosis, 2010, 213(2)∶415-421
[9] V. Yurchenko, S. Constant, M. Bukrinsky, Dealing with the family: CD147 interactions with cyclophilins [J]. Immunology, 2006, 117(3)∶301-309
[10] Nishioku T, Dohgu S, Koga M, et al. Cyclophilin A secreted from fibroblast-like synoviocytes is involved in the induction of CD147 expression in macrophages of mice with collagen-induced arthritis [J]. J Inflamm (Lond), 2012, 9(1)∶44-44
[11] Liu L, Li C, Cai C, et al. Cyclophilin A (CypA) is associated with the inflammatory infiltration and alveolar bone destruction in an experimental periodontitis [J]. Biochemical and biophysical research communications, 2010, 391(1)∶1000-1006
[12] Seizer P, Ochmann C, Schnberger T, et al. Disrupting the EMMPRIN (CD147)-cyclophilin A interaction reduces infarct size and preserves systolic function after myocardial ischemia and reperfusion [J]. Arteriosclerosis, thrombosis, and vascular biology, 2011, 31(6)∶1377-1386
[13] Seizer P, Schnberger T, Schtt M, et al. EMMPRIN and its ligand cyclophilin A regulate MT1-MMP, MMP-9 and M-CSF during foam cell formation [J]. Atherosclerosis, 2010, 209(1)∶51-57
[14] Seizer P, Geisler T, Bigalke B, et al. EMMPRIN and its ligand Cyclophilin A as novel diagnostic markers in inflammatory cardiomyopathy [J]. International journal of cardiology, 2013, 163(3)∶299-304
[15] Yang Y, Lu N, Zhou J, et al. Cyclophilin A up-regulates MMP-9 expression and adhesion of monocytes/macrophages via CD147 signalling pathway in rheumatoid arthritis [J]. Rheumatology, 2008, 47(9)∶1299-1310
[16] 王艳青,胡菊花,李颂,等.亲环素A在人根尖周炎中的表达与临床表现的相关性研究[J].牙体牙髓牙周病学杂志,2015,25(1)∶73-78
[17] 鄢雪川, 陈德平, 宿玉成.CBCT 和根尖放射线片诊断根尖周炎的对比性研究[J].口腔医学研究,2014,30(5)∶461-464
[18] Li M, Zhai Q, Bharadwaj U, et al. Cyclophilin A is overexpressed in human pancreatic cancer cells and stimulates cell proliferation through CD147 [J]. Cancer, 2006, 106(10)∶2284-2294
[19] Sousa NGK, Cardoso CRB, Silva JS, et al. Association of matrix metalloproteinase inducer (EMMPRIN) with the expression of matrix metalloproteinases-1,-2 and-9 during periapical lesion development [J]. Archives of Oral Biology, 2014∶944-953
[20] Liu L, Li C, Cai X, et al. The temporal expression and localization of extracellular matrix metalloproteinase inducer (EMMPRIN) during the development of periodontitis in an animal model [J]. Journal of periodontal research, 2010, 45(4)∶541-549
[21] Omi Y, Shibata N, Okamoto T, et al. The role of CD147 in the invasiveness of follicular thyroid carcinoma cells [J]. Thyroid, 2012, 22(4)∶383-394
[22] Kim H, Kim WJ, Jeon ST, et al. Cyclophilin A may contribute to the inflammatory processes in rheumatoid arthritis through induction of matrix degrading enzymes and inflammatory cytokines from macrophages [J]. Clinical Immunology, 2005, 116(3)∶217-224

基质金属蛋白酶诱导因子和亲环素A在人根尖囊肿和根尖肉芽肿中的表达及临床意义

Expression and Clinical Significance of CD147 and CypA in Periapical Granulomas and Radicular Cysts.

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