口腔医学研究

口腔医学研究 ›› 2017, Vol. 33 ›› Issue (1): 86-89.DOI: 10.13701/j.cnki.kqyxyj.2017.01.020

• 临床研究论著 • 上一篇    下一篇

白藜芦醇对脱矿牙本质基质影响的相关性研究

赵志香1,刘陆滨1*,袁雪明2   

  1. 1.佳木斯大学附属第二医院口腔医院牙体牙髓病二科 黑龙江 佳木斯 154002;
    2. 苏州市广济医院 江苏 苏州 215008
  • 收稿日期:2016-07-03 出版日期:2017-01-25 发布日期:2017-01-22
  • 通讯作者: 刘陆滨,E-mail: liulubin5873@163.com
  • 作者简介:赵志香(1992~ ),女,贵州人,硕士在读,主要从事口腔牙体牙髓病学的临床及基础研究工作。
  • 基金资助:
    佳木斯大学科学技术校级面上项目(编号:13Z1201558)
    佳木斯大学研究生科技创新项目(编号:LM2015_060)

Correlation Study on the Influence of Resveratrol on the Demineralized of Dentin Matrix.

ZHAO Zhi-xiang1, LIU Lu-bin1*, YUAN Xue-ming2   

  1. 1. Department of Endodontics, The Second Affiliated Hospital of Jiamusi
    University, Jiamusi 154002, China;
    2. Guangji Hospital of Suzhou, Jiangsu 215008, China.
  • Received:2016-07-03 Online:2017-01-25 Published:2017-01-22

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摘要： 目的:探讨白藜芦醇对基质金属蛋白酶-2活性及耐脱矿力的影响,以期为白藜芦醇的临床应用提供实验依据。方法:设置实验组白藜芦醇的浓度梯度:12.5 μmol/L、25 μmol/L、50 μmol/L、100 μmol/L、200 μmol/L、400 μmol/L,阳性对照组0.2%氯己定,阴性对照组去离子水。每组取10 μL与提取出来的牙源性mmp-2 30 μL混合孵育30 min,用体液基质金属蛋白酶-2活性比色法定量检测各组混合20 min后的酶活性,每组重复5次。 牙本质片经pH循环后,用激光共聚焦电子显微镜3D图像观察牙本质脱矿深度。结果:实验组白藜芦醇浓度在50 μmol/L以上时有明显抑制作用,且在100 μmol/L、200 μmol/L、400 μmol/L时的酶活性抑制率与阳性对照组间无显著性差异。激光共聚焦电子显微镜3D图像显示100 μmol/L Res的染色深度明显低于阴性对照组,统计学分析结果显示100 μmol/L Res的平均脱矿深度显著低于阴性对照组(P<0.05),与阳性对照组间并无显著性差异。结论:白藜芦醇可抑制基质金属蛋白酶-2活性及牙本质脱矿。

关键词: 白藜芦醇, 基质金属蛋白酶, 脱矿牙本质

Abstract: Objective: To discuss the effects of resveratrol on matrix metalloproteinase-2 (mmp-2) activity and demineralization resistance and to provide experimental evidence for the clinical application of resveratrol. Methods: The concentration gradients of resveratrol were 12.5 μmol/L, 25 μmol/L, 50 μmol/L, 100 μmol/L, 200 μmol/L, and 400 μmol/L in the experimental group. 0.2% chlorhexidine was selected as the positive control group and deionized water as the negative control group. 10 μL of each group was mixed with 30 μL extracted odontogenic mmp-2 and incubated for 30 min. Metalloproteinase-2 activity was quantitatively detected with body fluid matrix metalloproteinase-2 activity assay after 20 min, and each group was repeated five times. After going through the pH cycle, electron microscopy and laser scanning confocal 3D image were used to observe dentin demineralization depth. Results: Resveratrol with the concentration above 50 μmol/L showed significant inhibitory effect. And metalloproteinase-2 activity inhibition rate in groups with the concentration of 100 μmol/L, 200 μmol/L, and 400 μmol/L showed no significant difference compared to the positive group. Confocal electron microscopy 3D image displayed that Res dyeing depth in the 100 μmol/L group was significantly lower than that in the negative control group. And statistical analysis showed the same results (P<0.05). But there was no significant difference between 100 μmol/L group and the positive control group. Conclusion: Resveratrol can inhibit the activity of matrix metalloproteinase-2 and dentin demineralization.

Key words: Resveratrol, Matrix, metalloproteinases, Demineralized, dentin

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