口腔医学研究

口腔医学研究 ›› 2018, Vol. 34 ›› Issue (2): 148-151.DOI: 10.13701/j.cnki.kqyxyj.2018.02.010

• 口腔肿瘤学研究 • 上一篇    下一篇

MUC1-siRNA对人舌癌Tca细胞迁移、侵袭及PI3K-Akt信号通路的影响

邓轩, 阳芳*   

  1. 南华大学附属第二医院口腔科 湖南 衡阳 421001;
  • 收稿日期:2017-10-09 出版日期:2018-02-28 发布日期:2018-02-26
  • 通讯作者: 阳芳,E-mail:yangfang4203@163.com
  • 作者简介:邓轩(1985~ ),男,湖北荆州人,硕士,主治医师,主要从事舌癌发病机制与预防研究工作。
  • 基金资助:
    湖南省自然科学基金资助项目(编号:15JJ5026)

Influence of MUC1-siRNA on Migration, Invasion, and PI3K-Akt Signaling Pathway of Human Tongue Cancer Tca Cells

DENG Xuan, YANG Fang*   

  1. Department of Stomatology, Second Hospital Affiliated to Nanhua University, Hengyang 421001, China.
  • Received:2017-10-09 Online:2018-02-28 Published:2018-02-26

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摘要： 目的:探讨MUC1对舌癌Tca8113细胞迁移、侵袭及PI3K-Akt信号通路的影响。方法:构建MUC1特异性siRNA质粒,利用脂质体Lipo fectamineTM2000将MUC1-siRNA质粒转入舌癌Tca8113细胞,同时设置阴性对照组及空白组,分别检测3组MUC1 mRNA及蛋白表达水平,Tca8113细胞增殖活性及凋亡情况、迁移和侵袭能力,并检测PI3K-Akt通路关键蛋白PI3K、p-Akt、Akt表达水平。结果:经双酶切、PCR及测序验证,成功构建MUC1-siRNA表达载体;干扰组MUC1 mRNA及蛋白表达水平明显低于阴性对照组及空白组(P＜0.05);MUC1-siRNA可明显对Tca8113细胞抑制增值和促进凋亡,降低Tca8113细胞PI3K、p-Akt、Akt蛋白表达水平和迁移及侵袭能力。结论:MUC1-siRNA可明显抑制舌癌Tca8113细胞增殖、迁移及侵袭能力,促进Tca8113细胞凋亡,推测其机制可能与抑制PI3K-Akt信号通路表达有关。

关键词: 舌癌, RNA干扰, 黏蛋白1, 细胞迁移, 细胞侵袭

Abstract: Objective: To explore the influence of MUC1 on migration, invasion, and PI3K-Akt signaling pathway of human tongue cancer Tca8113 cells. Methods: MUC1 specific siRNA plasmid was established, and liposome Lipo fectamineTM2000 was applied to transfer MUC1-siRNA plasmid into tongue cancer Tca8113 cells. The expression levels of MUC1 mRNA and protein, cell proliferation and apoptosis, migration and invasion ability in three groups were detected. The expression levels of PI3K, p-Akt and Akt in the PI3K-Akt pathway were also detected. Results: After verified by double enzyme digestion, PCR and DNA sequencing, MUC1-siRNA expression vector was successfully constructed. MUC1 mRNA and protein expression levels in the interference group were significantly lower than those in the negative control group and blank control group (P<0.05). MUC1-siRNA could significantly inhibit the proliferation and promote the apoptosis of Tca8113 cells, and reduce the level of PI3K, p-Akt, Akt protein expression, migration, and invasion of cells. Conclusion: MUC1-siRNA can obviously inhibit the proliferation, migration, and invasion of tongue cancer Tca8113 cells, and promote cell apoptosis. It is speculated that the mechanism may relate to the the expression inhibition of PI3K-Akt.

Key words: Tongue cancer, RNA interference, Mucin 1, Cell migration, Cell invasion

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