载阿霉素介孔二氧化硅纳米粒对人舌癌Tca-8113细胞增殖及凋亡的影响

doi:10.13701/j.cnki.kqyxyj.2017.03.004

口腔医学研究 ›› 2017, Vol. 33 ›› Issue (3): 249-253.DOI: 10.13701/j.cnki.kqyxyj.2017.03.004

载阿霉素介孔二氧化硅纳米粒对人舌癌Tca-8113细胞增殖及凋亡的影响

李海琴¹，范增杰^1,2，刘斌^1*，余占海^1*

1. 兰州大学口腔医学院口腔修复科甘肃兰州 730000;
2. 中国科学院兰州化学物理研究所固体润滑国家重点实验室甘肃兰州 730000

收稿日期:2016-10-28 出版日期:2017-03-20 发布日期:2017-03-22
通讯作者: 刘斌,E-mail: liubkq@lzu.edu.cn;余占海,E-mail: yuzhanhai@lzu.edu.cn
作者简介:李海琴(1990~ ),女,甘肃省古浪县人,硕士,主要从事口腔修复学研究。
基金资助:
中央高校自由探索-面上项目(编号:lzujbky-2015-293)甘肃省卫生行业科研计划管理项目(编号:GWGL2014-06)

Effects of Doxorubicin-loaded Mesoporous Silica Nanoparticles on Proliferation and Apoptosis of Human Tongue Cancer Tca-8113 Cells.

LI Hai-qin¹, FAN Zeng-jie^1,2, LIU Bin^1*, YU Zhan-hai^1*.

1. School of Stomatology, Lanzhou University, Lanzhou 730000, China;
2. State Key Laboratory of Solid Lubrication, Lanzhou Insitute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China.

Received:2016-10-28 Online:2017-03-20 Published:2017-03-22

摘要/Abstract

摘要： 目的:探讨载阿霉素介孔二氧化硅纳米粒(MSNs@DOX)对体外培养的人舌癌Tca-8113 细胞增殖及凋亡的作用。方法:透射电镜(TEM)和氮吸附法对介孔二氧化硅纳米粒(MSNs)进行表征;紫外分光光度计检测阿霉素(DOX)释放行为;MTT法检测细胞增殖抑制作用;Hochest33342染色、流式细胞术检测细胞凋亡和周期分布;Western blot检测P53、Bcl-2、Bax 和Caspase-3 蛋白表达。结果:MSNs形态规则,分布均一,平均粒径、介孔孔径、比表面积和孔容分别为约30 nm,19.59682 nm、193.4296 m²/g、0.947625 cm³/g;MSNs细胞相容性良好;与单独DOX相比,载药组的细胞增殖抑制率及凋亡率更显著,且呈一定MSNs浓度依赖性(P<0.05);细胞明显阻滞于G0/G1期; P53、Bax、Caspase-3 表达显著升高,Bcl-2表达显著降低。结论:该载药纳米传输系统有望用于舌癌治疗。

关键词: 阿霉素, 介孔二氧化硅纳米粒, 舌癌, Tca-8113细胞

Abstract: Objective: To establish a drug delivery system based on mesoporous silica nanoparticles (MSNs) for its potential role in promoting the anticancer efficacy of doxorubicin (DOX) on Tca-8113 cells. Methods: MSNs were characterized by transmission electron microscope (TEM) and N2 adsorption/desorption method. The drug release profiles of DOX from MSNs were monitored by UV-Vis. The anticancer effect of MSNs@DOX was demonstrated by MTT assay, Hoechst 33342 staining, and flow cytometry. The related mechanism was explored by Western blot. Results: The particle size of MSNs was about 30 nm and its average pore size, surface area and pore volume were 19.59682 nm, 193.4296 m²/g and 0.947625 cm³/g, respectively. MSNs displayed good cytocompatibility. MSNs@DOX inhibited the proliferation of Tca-8113 cells, induced apoptosis remarkably and arrested cell cycle at G0/G1 checkpoints. Western blot revealed remarkable up-regulated expression of Bax, p53 and caspase-3 and down-regulated expression of Bcl-2. Conclusion: Such a doxorubicin delivery strategy might be promising in tongue cancer therapy.

Key words: Doxorubicin , Mesoporous silica nanoparticles , Tongue cancer, Tca-8113 cells

中图分类号:

R739.8

李海琴，范增杰，刘斌，余占海. 载阿霉素介孔二氧化硅纳米粒对人舌癌Tca-8113细胞增殖及凋亡的影响[J]. 口腔医学研究, 2017, 33(3): 249-253.

LI Hai-qin, FAN Zeng-jie, LIU Bin, YU Zhan-hai.. Effects of Doxorubicin-loaded Mesoporous Silica Nanoparticles on Proliferation and Apoptosis of Human Tongue Cancer Tca-8113 Cells.[J]. Journal of Oral Science Research, 2017, 33(3): 249-253.

参考文献

[1] 戴红良,葛树卿,楚明会,等.染料木黄酮通过抑制VEGF表达抑制人口腔癌TCA8113 细胞增殖[J].中国病理生理杂志, 2016,32(3)∶464-469
[2] Zheng J, Lee, HCM, bin Sattar MM, et al. Cardioprotective effects of epigallocatechin-3-gallate against doxorubicin-induced cardiomyocyte injury [J]. Eur J Pharmacol, 2011, 562(1)∶82-88
[3] S. Granados-Principal, N. El-azem, R. Pamplona, et al. Hydroxytyrosol ameliorates oxidative stress and mitochondrial dysfunction in doxorubicin-induced cardiotoxicity in rats with breast cancer, Biochem. Pharmacol. 90 (2014) 25e33
[4] Yousefpour P, Atyabi F, Farahani EV, et al. Polyanionic carbohydrate doxorubicin-dextran nanocomplex as a delivery system for anticancer drugs: in vitro analysis and evaluations [J]. Int J Nanomedicine, 2011, 6(12)∶1487-1496
[5] Jannesari M, Varshosaz J, Morshed M, et al. Composite poly(vinyl alcohol)/poly(vinyl acetate) electrospun nanofibrous mats as a novel wound dressing matrix for controlled release of drugs [J]. Int J Nanomedicine, 2011, 6(8)∶993-1003
[6] L. Dai, J. Li, B. Zhang, et al. Redox-Responsive Nanocarrier Based on Heparin End-Capped Mesoporous Silica Nanoparticles for Targeted Tumor Therapy in Vitro and in Vivo [J]. Langmuir, 2014, 30(14)∶7867-7877
[7] A. Sinha, A. Chakraborty, N.R. Jana, et al. Multifunctional Mesoporous Nanoparticle for Glucose-Responsive and Targeted Drug Delivery.ACS Appl. Mater [J]. Interfaces, 2014, 6(16)∶22183-22191
[8] B.S. Chang, D. Chen, Y. Wang, et al. Bioresponsive Controlled Drug Release Based on Mesoporous Silica Nanoparticles Coated with Reductively Sheddable Polymer Shell [J]. Chem. Mater, 2013, 25(5)∶574-585
[9] LAVASANIFAR A,SAMUEL J,KWON GS. Poly( ethyleneoxide) block-poly( Lamino acid) micelles or drug delivery [J]. Adv Drug deliv Rev, 2002, 54(2)∶169-190
[10] Lingjie W, Ming W, Yongyi Z, et al. Multifunctional PEG modified DOX loaded mesoporous silica nanoparticle@CuS nanohybrids as photo-thermal agent and thermal-triggered drug release vehicle for hepatocellular carcinoma treatment [J]. Nanotechnology, 2015, 26(2)∶025102
[11] Vallet-Regi M, Rmila A, del Real RP, et al. A new property of MCM-41: drug delivery system [J]. Chem Mater, 2001, 13(2)∶308-311
[12] Maher S, Kumeria T, Wang Y, et al. From The Mine to Cancer Therapy: Natural and Biodegradable Theranostic Silicon Nanocarriers from Diatoms for Sustained Delivery of Chemotherapeutics [J]. Adv Healthc Mater, 2016, 5(20)∶2667-2678
[13] Shen D, Yang J, Li X, et al. Biphase stratification approach to three-dimensional dendritic biodegradable mesoporous silica nanospheres [J]. Nano Lett, 2014, 14(2)∶923-932
[14] Sardan M, Yildirim A, Mumcuoglu D, et al. Noncovalent functionalization of mesoporous silica nanoparticles with amphiphilic peptides [J]. J Mater Chem, 2014, 2(15)∶2168-2174
[15] Rosenholm J M, Mamaeva V, Sahlgren C, et al. Nanoparticles in targeted cancer therapy: mesoporous silica nanoparticles entering preclinical development stage [J]. Nanomedicine(Lond), 2012, 10(1)∶111-120
[16] 孙国文,张笑佛,李田,等.Hedgehog信号通路效应蛋白在舌癌组织中的表达及意义[J].口腔医学研究,2015,31(4)∶393-396
[17] Beilu Zhang, Zhong Luo, Junjie Liu, et al. Cytochrome c end-capped mesoporous silica nanoparticles as redox-responsive drug delivery vehicles for liver tumor-targeted triplex therapy in vitro and in vivo [J]. Journal of Controlled Release, 2014, 192(2)∶192-201

载阿霉素介孔二氧化硅纳米粒对人舌癌Tca-8113细胞增殖及凋亡的影响

Effects of Doxorubicin-loaded Mesoporous Silica Nanoparticles on Proliferation and Apoptosis of Human Tongue Cancer Tca-8113 Cells.

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