Pik3cb在小鼠下颌第一磨牙牙胚发育不同阶段的表达研究

doi:10.13701/j.cnki.kqyxyj.2017.03.005

口腔医学研究 ›› 2017, Vol. 33 ›› Issue (3): 254-257.DOI: 10.13701/j.cnki.kqyxyj.2017.03.005

Pik3cb在小鼠下颌第一磨牙牙胚发育不同阶段的表达研究

张杨¹，柴纪华¹，任铭新^2*，王革^1*

1. 武汉大学口腔医学院口腔医学生物工程教育部重点实验室湖北武汉 430079;
2. 武汉大学基础医学院湖北武汉 430000

收稿日期:2016-11-10 出版日期:2017-03-20 发布日期:2017-03-22
通讯作者: 任铭新,E-mail:hnlyrmx@163.com
作者简介:张杨(1991~ ),男,黑龙江人,硕士在读,主要从事分子生物学相关研究工作。

Temporal and Spatial Expression of Pik3cb at Different Developmental Stages of Mouse Mandibular First Molar.

ZHANG Yang¹, CHAI Ji-hua¹, REN Ming-xin², WANG Ge^1*

1. Hubei MOST KLOS &KLOBM, School and Hospital of Stomatology, Wuhan University, Wuhan 430079, China;
2. Medical College, Wuhan University, Wuhan 430074, China

Received:2016-11-10 Online:2017-03-20 Published:2017-03-22

摘要/Abstract

摘要： 目的:检测Pik3cb在小鼠下颌第一磨牙牙胚发育不同阶段的表达分布及变化规律,进一步揭示Pik3cb对牙形态发生的影响。方法:制备原位杂交探针,及小鼠牙胚发育标志时间点的牙胚冰冻切片,通过原位杂交方法进一步显示Pik3cb在小鼠下颌第一磨牙牙胚发育不同时期的表达情况。结果:蕾状期Pik3cb明显表达于牙板上皮,帽状期开始大量表达于釉上皮与牙乳头,进入钟状期表达部位趋于集中,尤以内釉上皮及后续新形成硬组织周围的高柱状成釉细胞处最为明显。结论:Pik3cb在牙源性上皮及成釉细胞表达,并可能通过PI3K/PTEN/AKT/mTOR信号通路与成釉细胞瘤行为相关。

关键词: PI3K/PTEN/AKT/mTOR, 磨牙牙胚, 牙发育, 原位杂交(ISH), 成釉细胞瘤

Abstract: Objective: To detect the expression pattern and variation of Pik3cb at different developmental stages of mouse mandibular first molar. Methods: The probe for in situ hybridization was prepared. Mouse embryos at different times were obtained to get cryostat serial sections of tooth germ for ISH. Results: In bud period, Pik3cb obviously expressed in dental lamina epithelium. In bonnet period, the Pik3cb began to express in the enamel epithelium and dental papilla. In bell stage, the expression of Pik3cb was especially within the enamel epithelium and subsequently in the new formation of hard tissues surrounding the columnar cells into glaze. Conclusion: Pik3cb expresses in dental epithelial cells and ameloblasts and it is likely to associate with ameloblastoma through PI3K/PTEN/AKT/mTOR signaling pathway.

Key words: PI3K/PTEN/AKT/mTOR, Molar tooth germ , Tooth development, In situ hybridization(ISH)Ameloblastoma

中图分类号:

Q132

张杨，柴纪华，任铭新，王革. Pik3cb在小鼠下颌第一磨牙牙胚发育不同阶段的表达研究[J]. 口腔医学研究, 2017, 33(3): 254-257.

ZHANG Yang, CHAI Ji-hua, REN Ming-xin, WANG Ge. Temporal and Spatial Expression of Pik3cb at Different Developmental Stages of Mouse Mandibular First Molar.[J]. Journal of Oral Science Research, 2017, 33(3): 254-257.

参考文献

[1] Kumamoto, H. K. Ooya, Immunohistochemical detection of phosphorylated Akt, PI3K, and PTEN in ameloblastic tumors [J]. Oral Dis, 2007, 13(5)∶461-467
[2] Utermark T. The p110alpha and p110beta isoforms of PI3K play divergent roles in mammary gland development and tumorigenesis [J]. Genes Dev, 2012,26(14)∶1573-86
[3] Aberg, T., J. Wozney, I. Thesleff, Expression patterns of bone morphogenetic proteins (Bmps) in the developing mouse tooth suggest roles in morphogenesis and cell differentiation [J]. Dev Dyn, 1997, 210(4)∶383-96
[4] Assaraf-Weill N. Ameloblasts express type I collagen during amelogenesis [J]. J Dent Res, 2014, 93(5)∶502-7
[5] Tucker, A. P. Sharpe, The cutting-edge of mammalian development; how the embryo makes teeth [J]. Nat Rev Genet, 2004, 5(7)∶499-508
[6] Brook AH. Multilevel complex interactions between genetic, epigenetic and environmental factors in the aetiology of anomalies of dental development [J]. Arch Oral Biol, 2009, 54(1)∶S3-17
[7] Li J. BMP-SHH signaling network controls epithelial stem cell fate via regulation of its niche in the developing tooth [J]. Dev Cell, 2015, 33(2)∶125-35
[8] 梁鑫, 王.成纤维细胞生长因子及其受体在牙齿生长发育中的作用[J].口腔医学研究,2016,32(4)∶421-423
[9] Leevers, S.J., B. Vanhaesebroeck, M.D. Waterfield, Signalling through phosphoinositide 3-kinases: the lipids take centre stage [J]. Curr Opin Cell Biol, 1999, 11(2)∶219-225
[10] Carpenter CL. Purification and characterization of phosphoinositide 3-kinase from rat liver [J]. J Biol Chem, 1990, 265(32)∶19704-19711
[11] Papa A. Cancer-associated PTEN mutants act in a dominant-negative manner to suppress PTEN protein function [J]. Cell, 2014, 157(3)∶595-610
[12] Newton AC. L.C. Trotman, Turning off AKT: PHLPP as a drug target [J]. Annu Rev Pharmacol Toxicol, 2014, 54(5)∶537-558
[13] Nakao, Y. Fibroblast growth factors 7 and 10 are involved in ameloblastoma proliferation via the mitogen-activated protein kinase pathway. Int J Oncol, 2013, 43(5)∶1377-1384
[14] Li N. Expression of phosphorylated Akt/mTOR and clinical significance in human ameloblastoma [J]. Int J Clin Exp Med, 2015, 8(4)∶5236-5244
[15] Scheper MA. Expression and alterations of the PTEN / AKT / mTOR pathway in ameloblastomas [J]. Oral Dis, 2008, 14(6)∶561-568
[16] Kaye FJ. Clinical and radiographic response with combined BRAF-targeted therapy in stage 4 ameloblastoma [J]. J Natl Cancer Inst, 2015, 107(1)∶378-383

Pik3cb在小鼠下颌第一磨牙牙胚发育不同阶段的表达研究

Temporal and Spatial Expression of Pik3cb at Different Developmental Stages of Mouse Mandibular First Molar.

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