舌癌移植瘤活体成像的特点及应用

doi:10.11839/kqyxzh.2016.01.010

口腔医学研究 ›› 2016, Vol. 32 ›› Issue (1): 39-42.DOI: 10.11839/kqyxzh.2016.01.010

舌癌移植瘤活体成像的特点及应用

靳志萌¹,王通²,郝伟亮¹,杨立³,沈军^3*

1. 天津医科大学天津 300000;
2. 南开大学天津 300000;
3. 天津市口腔医院天津 300000

收稿日期:2015-07-29 出版日期:2016-01-28 发布日期:2016-03-21
通讯作者: 沈军,E-email:13902143866@163.com
作者简介:靳志萌(1988~),女,河南人,硕士在读,研究方向为舌癌淋巴结转移机制。

Characteristic and Application of Transplanted Tumor Imaging of Tongue Cancer.

JIN Zhi-meng¹, WANG Tong², HAO Wei-liang¹, YANG Li³, SHEN Jun³.

1. Tianjin Medical University, Tianjin 300000, China;
2. Nankai University, Tianjin 300000, China;
3. Tianjin Stomatological Hospital, Tianjin 300000, China

Received:2015-07-29 Online:2016-01-28 Published:2016-03-21

摘要/Abstract

摘要： 目的:建立稳定表达荧光素酶的舌癌细胞株并应用生物发光成像技术监测活体裸鼠舌癌侵袭发展过程。方法:应用脂质体将携带荧光素酶基因的质粒转染到人舌癌Tca83细胞株中,G418筛选出稳定表达荧光素酶的单克隆细胞株,扩增后接种于裸鼠,建立裸鼠舌体及皮下移植瘤模型。活体成像监测肿瘤的生长情况,HE染色病理分析移植瘤细胞的组织学特征。结果:获得了稳定的舌癌单克隆细胞株,该单克隆细胞株能够稳定表达荧光素酶;移植后获得稳定的动物模型,应用小动物活体成像系统能准确监测肿瘤细胞在体内的侵袭过程。结论:本研究成功的构建了稳定表达荧光素酶的单克隆细胞株和稳定的舌癌移植瘤动物模型,用于活体成像检测;为舌癌区域淋巴结转移机制的研究提供了新的研究方法。

关键词: 舌癌, 裸鼠, 荧光素酶, 活体成像, 生物发光

Abstract: Objective: To establish tongue cancer cell line and investigate the development of tongue cancer bioluminescence imaging in nude mice. Methods: Liposome plasmid carring the luciferase gene was transfected into Tca83 cells and G418 was used to select these cells. The cultured cells were transplanted into nude mice. Cancer cells was monitored and characterized by HE staining. Results: The monoclonal cell line expressing luciferase stably was obtained and monitored in vivo by bioluminescent imaging technology. Conclusion: This study provides a new way to investigate the mechanism of cancer development and metastasis.

中图分类号:

R739.8

靳志萌,王通,郝伟亮,杨立,沈军. 舌癌移植瘤活体成像的特点及应用[J]. 口腔医学研究, 2016, 32(1): 39-42.

JIN Zhi-meng, WANG Tong, HAO Wei-liang, YANG Li, SHEN Jun.. Characteristic and Application of Transplanted Tumor Imaging of Tongue Cancer.[J]. Journal of Oral Science Research, 2016, 32(1): 39-42.

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舌癌移植瘤活体成像的特点及应用

Characteristic and Application of Transplanted Tumor Imaging of Tongue Cancer.

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