口腔医学研究

口腔医学研究 ›› 2017, Vol. 33 ›› Issue (9): 958-961.DOI: 10.13701/j.cnki.kqyxyj.2017.09.012

• 基础研究论著 • 上一篇    下一篇

RNA干扰XBP1基因表达对口腔鳞状细胞癌生物学特性的影响研究

张睿1，杨艳秋2，钟诗语1，楚亚楠3*   

  1. 1. 成都医学院临床医学院 四川 成都 610500；
    2. 成都医学院第一附属医院实验中心 四川 成都 610500；
    3. 成都医学院基础医学院 四川 成都 610500
  • 收稿日期:2017-03-29 出版日期:2017-09-20 发布日期:2017-09-27
  • 通讯作者: 楚亚楠,E-mail:zhangrui198681@163.com
  • 作者简介:张睿(1986~ ),女,四川乐山人,学士,助教,主要从事口腔基础医学研究。
  • 基金资助:
    四川省教育厅科研项目(编号:15ZB0245)

Effects of Inhibition of XBP1 Gene Expression on Biological Characteristics of Oral Squamous Cell Carcinoma by RNA Interference

ZHANG Rui1, YANG Yan-qiu2, ZHONG Shi-yu1, CHU Ya-nan3*   

  1. 1. School of Clinical Medicine, Chengdu Medical College, Chengdu 610500, China;
    2. Experiment Center, the First Affiliated Hospital of Chengdu Medical College, Chengdu 610500, China;
    3. School of Basic Medical Sciences, Chengdu Medical College, Chengdu 610500, China.
  • Received:2017-03-29 Online:2017-09-20 Published:2017-09-27

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摘要： 目的:检测口腔鳞状细胞癌组织中X盒结合蛋白1(XBP1)的表达及观察抑制其表达后对人口腔鳞状细胞癌Tca8113增殖凋亡的影响,并探讨其机制。方法:RT-PCR检测42例口腔鳞状细胞癌及癌旁组织中XBP1基因的mRNA表达;NC-siRNA和XBP1-siRNA转染至Tca8113细胞内,不经任何处理的细胞作为空白对照组,48 h后,CCK8实验、流式细胞仪分别检测XBP1基因转染对细胞增殖、凋亡的影响;Western blot检测Cleaved caspase3、β-catenin、Cyclin D1蛋白表达的变化。结果:口腔鳞状细胞癌中XBP1基因的mRNA表达显著高于癌旁组织(P<0.01);转染XBP1-siRNA能够显著降低XBP1的蛋白表达,降低细胞的存活率,促进细胞的凋亡,上调Cleaved caspase3蛋白表达,下调β-catenin、Cyclin D1蛋白表达(P<0.01)。结论:RNA干扰沉默XBP1基因表达可通过抑制Wnt/β-catenin信号通路降低Tca8113增殖及诱导细胞凋亡。

关键词: RNA干扰, XBP1基因, 口腔鳞状细胞癌, 增殖, 凋亡, Wnt/β-catenin信号通路

Abstract: Objective: To detect the expression of X box binding protein 1 (XBP1) in human oral squamous cell carcinoma (OSCC) and to investigate the effect of inhibition the expression of XBP1 on proliferation and apoptosis of Tca8113. Methods: The expressions of XBP1 gene in the OSCC and paracancerous tissue were detected by RT-PC. NC-siRNA and XBP1-siRNA were transfected into Tca8113 cells, and the cells without any treatment were set the control group. The cell proliferation and apoptosis were detected after XBP1 gene transfection for 48h by CCK8 test and flow cytometry. The cleaved caspase3, β-catenin, and Cyclin D1 protein expression were detected by Western blot. Results: The mRNA expression of XBP1 gene in OSCC was significantly higher than that in paracancerous tissues (P<0.01). The expression of XBP1 protein and the cell viability were significantly decreased. Cell apoptosis were promoted. The protein expression of cleaved Caspase3 was increased, but the expressions of β-catenin and Cyclin D1 were decreased after XBP1-siRNA transfection (P<0.01). Conclusion: Silencing XBP1 gene expression by RNA interference can inhibit the proliferation of Tca8113 and induce apoptosis by inhibiting the Wnt/ β-catenin signaling pathway.

Key words: RNA interference , XBP1 gene, Oral squamous cell carcinoma, Proliferation, Apoptosis Wnt/β-catenin signaling pathway

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