口腔医学研究 ›› 2018, Vol. 34 ›› Issue (11): 1217-1222.DOI: 10.13701/j.cnki.kqyxyj.2018.11.017

• 口腔正畸学研究 • 上一篇    下一篇

Adrb2激动剂、拮抗剂对大鼠牙移动中牙槽骨微结构改建的影响研究

唐小莹, 龙杨, 袁小平*   

  1. 西南医科大学附属口腔医院正畸科 四川 泸州 646000
  • 收稿日期:2018-05-28 出版日期:2018-11-28 发布日期:2018-11-23
  • 通讯作者: 袁小平,E-mail: 90300297@qq.com
  • 作者简介:唐小莹(1993~ ),女,四川资阳人,硕士,主要从事口腔正畸学研究工作。
  • 基金资助:
    泸州市政府-四川医科大学联合项目[编号:2015LZCYD-S05(1/12) ]

Effects of Adrb2 Agonists and Antagonists on Alterations of Alveolar Bone Remodeling and Expression of RANKL in Rat Tooth during Tooth Movement

TANG Xiao-ying, LONG Yang,YUAN Xiao-ping*   

  1. Department of Orthodontics, Hospital of Stomatology, Southwest Medical University. Luzhou 646000, China.
  • Received:2018-05-28 Online:2018-11-28 Published:2018-11-23

摘要: 目的:讨论β-2肾上腺素受体(β-2 adrenergic receptor,Adrb2)激动剂、拮抗剂对大鼠正畸牙移动牙槽骨微结构改建、破骨细胞生成的影响。方法:64只雌性SD大鼠随机分成异丙肾上腺素组、普萘洛尔组、阴性对照组及生理盐水(stroke-physiological saline solution,SPSS)组。建立大鼠正畸牙移动模型,按体重分别于腹腔注射相应药物,0 d、7 d、14 d、21 d处死大鼠,游标卡尺测量第一磨牙移动距离, TRAP染色观察破骨细胞数量,Micro-CT评价牙槽骨微结构变化。结果:牙移动距离、破骨细胞计数均表现为:异丙肾上腺素组>生理盐水组>普萘洛尔组>阴性对照组,差异有统计学意义(P<0.05),Micro-CT扫描结果发现异丙肾上腺素能够降低骨体积分数(BV/TV)与骨小梁密度(MTPD),升高骨小梁分离度(Tb.sp),而普萘洛尔则刚好相反。结论:肾上腺素能够促进压力侧牙槽骨的吸收、破骨细胞的生成,使牙槽骨变得疏松而利于正畸牙的移动;普萘洛尔抑制破骨细胞的生成、增加牙槽骨骨密度而减缓牙齿移动。

关键词: β-2肾上腺受体, 正畸牙移动, 破骨细胞, 牙槽骨改建

Abstract: Objective: To evaluate the effect of agonist and inhibitor of Adrb2 on the alveolar bone micro-structure reconstruction and osteoclast proliferation during the movement of orthodontic teeth in rats. Methods: 64 female SD rats were randomly divided into four groups: isoproterenol group, propranolol group, negative control group, and saline group. The rat orthodontic teeth movement models were established. The rats were injected corresponding reagents according to weight and were sacrificed on day 0, 7, 14, and 21. The movement distance of first molar of rats was measured by Vernier caliper. The numbers of osteoclasts were observed through TRAP staining. The change of micro-structure of alveolar bone was evaluated by Micro-CT. Results: The trends of the distance of teeth movement and numbers of osteoclast were the same: isoproterenol group> saline group> propranolol group> negative control group (P<0.05). Absorption lacuna increasing at the pressure side of alveolars could be observed through HE staining in isoproterenol group, saline group, and propranolol group and the isoproterenol group was the most. The result of Micro-CT scan showed that isoproterenol could reduce BV/TV and MTPD, and increase Tb.sp, while propranolol was on the opposite. Conclusion: Isoproterenol could promote the resorption of alveolar bone at the pressure side and increase the osteoclast numbers so that the alveolar bone became looser which was beneficial to the movement of orthodontic tooth. On the contrary, propranolol could inhibit the proliferation of osteoclast, increase the density of alveolar, and slow down the movement of tooth.

Key words: β2 adrenergic receptor, Orthodontic tooth movement, Osteoclast, Alveolar bone remodeling