口腔医学研究

口腔医学研究 ›› 2018, Vol. 34 ›› Issue (9): 960-963.DOI: 10.13701/j.cnki.kqyxyj.2018.09.010

• 口腔正畸学研究 • 上一篇    下一篇

牙槽骨微穿孔术促进大鼠正畸牙移动的机制研究

赵云鹤,李娇,雷浪*,李厚轩   

  1. 南京大学医学院附属口腔医院/南京市口腔医院正畸科,
    南京大学医学院附属口腔医院中心实验室 江苏 南京 210008
  • 收稿日期:2018-03-08 出版日期:2018-09-28 发布日期:2018-09-25
  • 通讯作者: 雷浪,E-mail:leilangdental@163.com
  • 作者简介:赵云鹤(1989~ ),女,山西人,硕士研究生,主要从事正畸临床及基础相关研究。
  • 基金资助:
    南京市医学科技发展项目(编号:YK15155、JQX15036)

Mechanism of Micro-osteoperforation-accelerated Orthodontic Tooth Movement in Rats.

ZHAO Yun-he, LI Jiao, LEI Lang*, LI Hou-xuan.   

  1. Department of Orthodontics, Nanjing Stomatological Hospital, Medical School of Nanjing University, Central Laboratory of Stomatology, Institute and Hospital of Stomatology, Nanjing University Medical School, Nanjing University, Nanjing 350002, China.
  • Received:2018-03-08 Online:2018-09-28 Published:2018-09-25

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摘要： 目的: 研究牙槽骨微穿孔技术(micro-osteoperforation,MOP)对牙移动过程中牙周组织改建的影响及其机制。方法: 以16只8周龄、无特定病原体(specific pathogen free,SPF)级健康雌性SD大鼠为研究对象,在一侧上颌第一磨牙近中行MOP,对侧不行MOP作为对照;以上颌中切牙为支抗,30 g力牵引上颌第一磨牙近中移动。7 d后处死大鼠,micro-CT测量上颌第一磨牙牙齿移动距离和牙槽骨的骨密度及骨体积分数;行苏木精-伊红染色(hematoxylin-eosin staining,HE)和免疫组织化学染色分析大鼠牙周组织的改建。结果: MOP加速了正畸牙移动,MOP+正畸加力组上颌第一磨牙移动距离为(420.82±175.58)μm,明显高于单纯正畸加力组(224.87±85.64)μm;对第一磨牙牙根周围骨密度及骨体积分数的分析结果显示,MOP+正畸加力组低于正畸组;HE染色结果显示,正畸加力后,第一磨牙压力侧牙周膜压缩变窄,张力侧牙周膜增宽;免疫组织化学染色结果显示,MOP+正畸加力组牙周组织内白细胞介素-6(interleukin-6,IL-6)表达水平高于单纯正畸加力组。结论: 牙槽骨MOP增强了牙周组织促炎细胞因子表达水平,促进了正畸牙移动速度。

关键词: 微穿孔, 正畸牙移动, 细胞因子

Abstract: Objective: To study the effect and mechanism of alveolar bone micro-osteoperforation (MOP) technique on periodontal tissue remodeling during tooth movement. Methods: Sixteen female, eight-week-old SPF Sprague Dawley rats were included in the study. MOP was performed on one side but not on the other control side. Upper 1st molar was protracted by 30 g orthodontic force with incisors being anchorage teeth. After 7 days, animals were sacrificed and the micro-CT scanning was performed to measure the tooth movement distance and bone density as well as bone volume fraction around the maxillary first molar. HE staining and immunohistochemical staining were utilized to analyze the alveolar bone remodeling in the periodontal tissue. Results: Orthodontic tooth movement was accelerated after performing MOP (420.82±175.58) μm mesial movement in the MOP + orthodontic force group and (224.87±85.64) μm in orthodontic force alone group. In addition, a more decreased bone mineral density and bone volume/total volume was observed in the MOP + orthodontic force group. Narrow periodontal membrane was observed on the pressure side while wide periodontal membrane was found on the tension side. Moreover, increased IL-6 level was observed in the periodontal tissues of MOP + orthodontic force group. Conclusion: MOP enhances the expression of proinflammatory cytokine in periodontal tissues and promotes the orthodontic tooth movement.

Key words: Micro-osteoperforation, Orthodontic tooth movement , Cytokine