口腔医学研究

口腔医学研究 ›› 2018, Vol. 34 ›› Issue (5): 558-562.DOI: 10.13701/j.cnki.kqyxyj.2018.05.023

• 其他研究 • 上一篇    下一篇

同种异体骨复合骨髓间充质干细胞用于髁突缺损修复的实验性研究

黄硕1, 李永锋2, 谭新颖3, 王宏4, 张安东2, 胡敏2, 刘昌奎1*   

  1. 1. 西安医学院 陕西 西安 710021;
    2. 中国人民解放军总医院 北京 100853;
    3. 解放军总医院三〇四临床部 北京 100000;
    4. 辽宁医学院附属第二医院 辽宁 锦州 121000
  • 收稿日期:2018-01-25 出版日期:2018-05-28 发布日期:2018-05-29
  • 通讯作者: 刘昌奎,E-mail:2727510502@qq.com
  • 作者简介:黄硕(1987~ ),男,河北保定人,硕士,助教,主要 从事口腔颌面外科颞下颌关节疾病的研究。
  • 基金资助:
    国家自然科学基金(编号:81470726)
    西安医学院2015年青年科研基金项目(编号:
    2015QN12)

Study on Repair of Condyle Defect by Allograft Bone Combined with Marrow Mesenchymal Stem Cells.

HUANG Shuo1, LI Yong-feng2, TAN Xin-ying3, WANG Hong4, ZHANG An-dong2, HU Min2, LIU Chang-kui1*   

  1. 1. Xi'an Medical College, Xi'an 710021, China;
    2. General Hospital of PLA, Beijing 100853, China;
    3. Clinical Department, 304 General Hospital of PLA, Beijing 100000, China;
    4. Affiliated Second Hospital, Liaoning Medical College. Jinzhou 121000, China
  • Received:2018-01-25 Online:2018-05-28 Published:2018-05-29

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摘要： 目的:运用组织工程的方法,以同种异体冻干骨支架复合骨髓间充质干细胞修复髁突缺损的重建方案,为临床单侧髁突缺损患者的修复提供实验依据。方法:12只12月龄比格犬,自髁突颈部切除所有动物左侧髁突,制造单侧髁突缺损的动物模型,随机分为两组,实验组用同种异体冻干骨支架复合骨髓间充质干细胞修复重建,对照组仅用同种异体冻干骨修复重建。分别于术后4、12、24周观察开口度变化,24周时进行螺旋CT扫描,然后处死动物,取标本行大体观察、Micro-CT扫描和组织学检测。结果:实验组和对照组所有动物同种异体冻干骨均能与自体骨愈合,24周时2组动物张口度基本正常,髁突形态无明显改变,实验组动物同种异体骨几乎全部被自体骨替代,对照组中,新骨主要在同种异体骨与自体骨结合部形成,实验组骨密度及成骨质量较对照组好(P<0.05)。结论:同种异体骨支架复合骨髓间充质干细胞能加速成骨,术后24周髁突形态变化不大,功能恢复良好,为进一步研究同种异体骨支架复合BMMSCs对髁突缺损的临床修复打下基础。

关键词: 同种异体冻干骨, 骨髓间充质干细胞, 髁突缺损, 组织工程, 修复重建

Abstract: Objective: To reconstruct condyle defect by allogeneic freeze-dried bone scaffold combined with bone marrow mesenchymal stem cells so as to provide experimental evidence for repairing of unilateral condyle defect. Methods: The left condyles from the condyle neck of 12 one year old beagles were cut off to prepare animal model of unilateral condyle defect. Then the animals were divided into two groups randomly: the experimental group with allergenic freeze-dried bone scaffold combined with bone marrow mesenchymal stem cells and the control group only with allergenic freeze-dried bone. The changes of opening degree were evaluated 4, 12, and 24 weeks after operation, and the morphological changes of condyle were observed. The animals were killed 24 weeks after the operation. The general observation, spiral CT scanning, micro-CT scanning, and histological examination were performed. Results: All allogeneic freeze-dried bone in the experimental group and control group could heal. After 24 weeks, the mouth opening degree of two groups of animals became almost normal. The condyle shape had no obvious change. The allograft bone in the experimental group was almost completely replaced by autogenous bone. In the control group, the new bone was formed mainly in the joint of the allograft bone and the autogenous bone. The bone mineral density and the quality of osteogenesis in the experimental group were better than those of the control group (P<0.05). Conclusion: Allogeneic bone scaffold combined with bone marrow mesenchymal stem cells can accelerate bone formation. Condylar shape changes little and the function recovery is good after 24 weeks. It lays a foundation for further study of allograft bone combined with bone marrow mesenchymal stem cells for clinical repair　of condyle defects.

Key words: Allogeneic freeze-dried bone, Bone marrow mesenchymal stem cells, Condyle defect, Tissue engineering, Reconstruction