口腔医学研究

口腔医学研究 ›› 2020, Vol. 36 ›› Issue (12): 1162-1167.DOI: 10.13701/j.cnki.kqyxyj.2020.12.018

• 颌面外科学研究 • 上一篇    下一篇

基质血管成分联合PRF辅助自体脂肪移植的动物实验研究

龙静, 黄谢山*, 潘小萌, 廖军   

  1. 中南大学湘雅医学院附属海口医院口腔中心,海南省口腔医学口腔颌面外科 海南 海口 570208
  • 收稿日期:2020-07-09 出版日期:2020-12-28 发布日期:2020-12-28
  • 通讯作者: *黄谢山,E-mail:hnsun3@126.com
  • 作者简介:龙静(1993~ ),女,湖南岳阳人,硕士,医师,主要从事于口腔整形修复方面工作。
  • 基金资助:
    海南省自然科学基金面上项目(编号:818MS138)

Effect of Combination of Stromal Vascular Fraction and PRF on Autogenous Fat Transplantation: An Animal Experimental Study

LONG Jing, HUANG Xieshan*, PAN Xiaomeng, LIAO Jun   

  1. Stomatology Center, Haikou Hospital Affiliate to Central South University Xiangya Medical School & Hainan Provincial Stomatology Center, Haikou 570208,China
  • Received:2020-07-09 Online:2020-12-28 Published:2020-12-28

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摘要： 目的:探讨基质血管成分联合PRF对兔自体脂肪吸收率的影响及脂肪颗粒与PRF的最佳质量比例。方法:采用双侧兔耳软组织缺损模型。在不同观察点比较各组移植物体积、成活率、质量等,对标本行HE染色、电镜扫描等比较各组移植物中脂肪细胞排列、形态以及数量的差别。结果:单纯脂肪移植组有2例术区表皮呈瘢痕愈合,余未见明显异常;随着观察时间的增加,移植物体积、成活率、质量均逐渐减少,当脂肪颗粒与PRF质量比为3∶1时,移植物存活率、质量及体积均最大;随着时间的增加,PRF缓慢释放,组织学观察显示各组脂肪细胞呈不同程度改建,B组脂肪细胞形态大小更均一,排列更紧密且相同视野完整脂肪细胞数量最多。结论:基质血管成分联合PRF能够提高自体脂肪组织移植后的存活率;脂肪颗粒与PRF的质量比为3∶1时其移植物存活率最高。

关键词: PRF, 基质血管成分, 自体脂肪移植, 组织工程

Abstract: Objective: To investigate the effect of stromal vascular fraction combined with PRF on the absorptivity of autogenous fat transplantation and the best ratio of adipose granules to PRF. Methods: The soft tissue defect model of bilateral rabbit ears was used. The volume, survival rate, and quality of the grafts were compared at different observation points. The arrangement, morphology, and quantity of adipocytes in each group were compared by HE staining and electron microscope scanning. Results: In the simple fat tissue transplantation group, there were 2 cases with cicatrization of the epidermis in the operation area, and no obvious abnormality was found in the rest. With the increase of observation time, the survival rate, mass, and volume of the grafts were gradually reduced. When the mass ratio of fat particles to PRF was 3∶1, the survival rate, mass, and volume of the grafts were the largest. With the increase of time, the PRF was slowly released, and histological observation showed that the fat cells in each group were not abnormal. With the same degree of reconstruction, the number of intact adipocytes in group B was the most, and the shape and size of adipocytes in group B were more uniform. Conclusion: The combination of PRF and stromal vascular fraction can reduce the absorption rate of autogenous adipose tissue and improve the survival rate of the graft. When the mass ratio of adipose granules to PRF was 3∶1, the graft survival rate was the highest.

Key words: PRF, stromal vascular fraction, autogenous fat transplantation, tissue engineering