C57BL/6J小鼠腭发育过程中电信号的时空变化规律

doi:10.13701/j.cnki.kqyxyj.2021.08.018

口腔医学研究 ›› 2021, Vol. 37 ›› Issue (8): 755-760.DOI: 10.13701/j.cnki.kqyxyj.2021.08.018

C57BL/6J小鼠腭发育过程中电信号的时空变化规律

段世均¹, 董书侠¹, 郑谦¹, 伍俊^2*

1. 口腔疾病研究国家重点实验室,国家口腔疾病临床医学研究中心,四川大学华西口腔医院唇腭裂外科四川成都 610041;
2. 口腔疾病研究国家重点实验室,国家口腔疾病临床医学研究中心,四川大学华西口腔医院口腔颌面外科四川成都 610041

发布日期:2021-08-11
通讯作者: 伍俊,E-mail:934963765@qq.com
作者简介:段世均(1985~ ),男,重庆人,博士,助理研究员,研究方向:唇腭裂遗传机制研究。
基金资助:
国家自然科学基金(编号:81200447)

Spatio-temporal Variational Rules of Electric Signal in Palate Development of C57BL/6J Mouse

DUAN Shijun¹, DONG Shuxia¹, ZHENG Qian¹, WU Jun^2*

1. State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Department of Cleft Lip and Plate, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China;
2. State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Department of Oral and Maxillofacial Surgery, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China

Published:2021-08-11

摘要/Abstract

摘要： 目的: 探讨C57BL/6J小鼠腭发育过程中生物电信号的时空变化规律,以及膜电位变化对腭发育的影响。方法: 在E12.5、E13.5、E14.5、E15.5断颈处死孕鼠获取腭胚突,使用DiBAC4(3)进行染色,观察腭发育过程中电信号的变化。然后进行体外培养,10 μmol/L HMR1098连续培养24、48、72 h后,观察腭融合的形态改变,通过免疫荧光染色检测TGF-β3的表达。结果: 腭胚突的口腔上皮、鼻腔上皮、中嵴上皮在腭发育的4个时期中均存在电信号;中嵴上皮的电信号强度随着腭形成逐渐减弱,上抬期最强,至融合期最终消失。除上抬期外,口腔上皮的电信号强度始终强于鼻腔上皮。HMR1098处理组的腭胚突仍能接触融合,但其形态较其余两组细长、尖锐;处理24 h后,腭胚突TGF-β3的表达明显高于其余两组,随着处理时间的延长,TGF-β3表达逐渐减弱直至消失。结论: 腭发育过程中存在电信号的时空变化,内源性电场的改变可以影响腭的发育。

关键词: 内源性电场, 腭发育, DiBAC4(3), HRM1098, 转化生长因子-β3

Abstract: Objective: To explore the spatio-temporal variational rules of electric signal in palate development of C57BL/6J mice, and assess the membrane potential changes of palate after selective inhibited KATP channel. Methods: DiBAC4(3) was used to trace the electric signal of embryonic palate through conversion from biological signal to optical signal with histological examination. Then the embryonic palate was cultured in vitro and treated with a selective K(ATP) channel inhibitor (HMR1098) for 24, 48, and 72 hours. The expression levels of TGF-β3 during palatogenesis were examined by immunohistochemistry analysis. Results: The oral, nasal, and medial edge epithelium existed obvious electrical signal during the four palatal development phases. The electrical signal intensity of medial edge epithelium decreased gradually along with palatogenesis. After treated by HMR1098, the palatal process could still elevate, contact, and fuse in vitro, however, the form of palatal process was slenderer and sharper. The TGF-β3 was strongly expressed in the oral, nasal, and medial edge epithelium after treated for 24h. With the increase of the HMR1098 treatment time, the expression of TGF-β3 trended to decline, eventually disappear. Conclusion: There were spatio-temporal variation of electric signal during palatogenesis, and the endogenous electrical fields affected the development of palate.

Key words: electrical signal, palatal developmen, DiBAC4(3), HRM1098, TGF-β3

段世均, 董书侠, 郑谦, 伍俊. C57BL/6J小鼠腭发育过程中电信号的时空变化规律[J]. 口腔医学研究, 2021, 37(8): 755-760.

DUAN Shijun, DONG Shuxia, ZHENG Qian, WU Jun. Spatio-temporal Variational Rules of Electric Signal in Palate Development of C57BL/6J Mouse[J]. Journal of Oral Science Research, 2021, 37(8): 755-760.

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C57BL/6J小鼠腭发育过程中电信号的时空变化规律

Spatio-temporal Variational Rules of Electric Signal in Palate Development of C57BL/6J Mouse

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