壳聚糖海藻酸钠复合海绵治疗大鼠干槽症的研究

doi:10.13701/j.cnki.kqyxyj.2021.08.019

口腔医学研究 ›› 2021, Vol. 37 ›› Issue (8): 761-765.DOI: 10.13701/j.cnki.kqyxyj.2021.08.019

壳聚糖海藻酸钠复合海绵治疗大鼠干槽症的研究

崔飞艳^1,2, 李蕾^1,3, 李娜¹, 袁壮^1,2, 孟箭^1,2,3*

1. 徐州医科大学徐州临床学院徐州市中心医院江苏徐州 221000;
2. 徐州医科大学口腔医学院江苏徐州 221000;
3. 潍坊医学院口腔医学院山东潍坊 261053

发布日期:2021-08-11
通讯作者: 孟箭,E-mail:mrocket@126.com
作者简介:崔飞艳(1995~ ),女,江苏宿迁人,硕士在读,住院医师,研究方向:牙槽外科。
基金资助:
国家自然科学基金(编号:31700814)江苏省卫生计生委科研资助项目(编号:H2017080)徐州市医学创新(技术攻关)团队(编号:XWCX201604)徐州市科技局重点研发项目(编号:KC17196)

Application of Chitosan Alginate Composite Sponge for Treatment of Dry Socket in Rats

CUI Feiyan^1,2, LI Lei^1,3, LI Na¹, YUAN Zhuang^1,2, MENG Jian^1,2,3*

1. Affiliated Xuzhou Clinical College of Xuzhou Medical University, Xuzhou Central Hospital, Xuzhou 221000, China;
2. School of Stomatology,Xuzhou Medical University, Xuzhou 221000, China;
3. Weifang Medical College Stomatology College, Weifang 261053, China

Published:2021-08-11

摘要/Abstract

摘要： 目的: 研究壳聚糖海藻酸钠复合海绵在大鼠干槽症中的治疗作用,以及与碘仿纱条对比,为临床应用提供依据。方法: 制作壳聚糖海藻酸钠复合海绵,行体外抑菌实验。将45只SD雄性大鼠拔除上颌第一磨牙感染干槽症,随机分为3组:A组空白组、B组壳聚糖海藻酸钠复合海绵组、C组:碘仿纱条组。观察并记录治疗后创口愈合情况,取上颌骨固定脱钙,HE染色进行组织病理学观察,免疫组化检测各组牙龈IL-6和IL-10的表达差异。结果: 壳聚糖海藻酸钠复合海绵结构均匀,有抑菌作用。与A组对比,肉眼观察、组织病理学观察及免疫组化结果均显示B组愈合良好,炎症控制(P＜0.05),且与C组无统计学差异(P＞0.05)。结论: 壳聚糖海藻酸钠复合海绵能够抗菌、消炎,治疗大鼠干槽症,且能达到与碘仿纱条相当的治疗效果。

关键词: 干槽症, 壳聚糖, 海藻酸钠, 复合海绵

Abstract: Objective: To study the therapeutic effect of chitosan alginate sponge in dry socket of rats and to provide basis for clinical application. Methods: Chitosan sodium alginate composite sponge was prepared and performed in vitro bacteriostatic test. 45 SD male rats were extracted the maxillary first molar and infected with dry socket, which were randomly divided into three groups: Group A, blank group, Group B, chitosan alginate composite sponge group, and Group C, iodine imitation yarn group. The wound healing was observed and recorded after treatment. The maxillary bone was removed and decalcified. HE staining was used for histopathological observation, and the expression of gingival IL-6 and IL-10 was detected by immunohistochemistry. Results: Compared with Group A, Group B had better healing and inflammation control observed by naked eye, histopathological analysis, and immunohistochemistry analysis (P<0.05), and there was no difference between Group B and Group C (P＞0.05). Conclusion: Chitosan sodium alginate composite sponge has antibacterial and anti-inflammatory property. The treatment of rat dry socket with sponge can achieve the same therapeutic effect as iodine imitation yarn.

Key words: dry socket, chitosan, sodium alginate, composite sponge

崔飞艳, 李蕾, 李娜, 袁壮, 孟箭. 壳聚糖海藻酸钠复合海绵治疗大鼠干槽症的研究[J]. 口腔医学研究, 2021, 37(8): 761-765.

CUI Feiyan, LI Lei, LI Na, YUAN Zhuang, MENG Jian. Application of Chitosan Alginate Composite Sponge for Treatment of Dry Socket in Rats[J]. Journal of Oral Science Research, 2021, 37(8): 761-765.

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壳聚糖海藻酸钠复合海绵治疗大鼠干槽症的研究

Application of Chitosan Alginate Composite Sponge for Treatment of Dry Socket in Rats

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