口腔医学研究

口腔医学研究 ›› 2018, Vol. 34 ›› Issue (8): 828-831.DOI: 10.13701/j.cnki.kqyxyj.2018.08.008

• 龋病牙髓病学研究 • 上一篇    下一篇

羧甲基壳聚糖-溶菌酶纳米凝胶稳定无定形磷酸钙促进脱矿牙釉质再矿化

宋金花1, 刘宗仁2, 刘文超3, 张旭1, 李彦秋1*   

  1. 1. 天津医科大学口腔医院综合科 天津 300070;
    2. 天津医科大学总医院滨海医院口腔科 天津 300070;
    3. 武警后勤学院附属医院口腔科 天津 300162
  • 收稿日期:2017-12-11 出版日期:2018-08-28 发布日期:2018-08-23
  • 通讯作者: 李彦秋,E-mail:13642074786@163.com
  • 作者简介:宋金花(1991~ ),女,山东人,硕士,主要从事口腔材料学研究。
  • 基金资助:
    国家自然科学基金(编号:81571016)

Nanogels of Carboxymethyl Chitosan and Lysozyme Encapsulated Amorphous Calcium Phosphate to Promote the Remineralization of Demineralized Enamel

SONG Jin-hua1, LIU Zong-ren2, LIU Wen-chao3, ZHANG Xu1, LI Yan-qiu1*   

  1. 1. Oral Comprehensive Department, Stomatology Hospital, Tianjin Medical University, Tianjin 300070, China;
    2. Department of Stomatology, Binhai Hospital, Tianjin Medical University, Tianjin 300070, China;
    3. Department of Stomatology, Affiliated Hospital of Armed Police Logistics College, Tianjin 300162, China
  • Received:2017-12-11 Online:2018-08-28 Published:2018-08-23

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摘要： 目的:制备羧甲基壳聚糖(CMC)-溶菌酶(LYZ)凝胶,稳定无定形磷酸钙(ACP)进而促进脱矿牙釉质再矿化。方法:首先制备CMC/LYZ-ACP纳米凝胶,利用透射电镜(TEM)观察凝胶的形貌,X射线能谱元素成像分析技术(EDX-Mapping)进行关键元素的定量及分布的分析。然后将纳米凝胶涂抹于酸蚀的牙釉质表面,利用扫描电镜(SEM)、纳米压痕实验(nano-idention)对脱矿牙釉质再矿化效果进行评估,并与人工唾液组进行对照。结果:CMC/LYZ-ACP纳米凝胶为粒径约50~300 nm的致密的球形颗粒。扫描电镜下可见脱矿后的釉质表面有新的晶体形成,与对照组相比,实验组的硬度及弹性模量更接近正常牙釉质。结论:CMC/LYZ-ACP纳米凝胶可以用于脱矿牙釉质的再矿化,属于新型的仿生再矿化材料。

关键词: 釉质矿化, 羧甲基壳聚糖, 溶菌酶, 无定形磷酸钙

Abstract: Objective: To stabilize amorphous calcium phosphate (ACP) and further promote the remineralization of demineralized enamel by preparation of carboxymethyl chitosan (CMC)-lysozyme (LYZ) gel. Methods: Firstly, CMC/LYZ-ACP nanogels were prepared. The morphology was characterized by transmission electron microscopy (TEM). Distribution and quantity of elements in CMC/LYZ-ACP nanogels were determined by element mapping and energy dispersive X-Ray spectroscopy (EDX). Then, the nanogel was applied to the surface of the etched enamel. Scanning electron microscopy (SEM) images and nanoindentation were applied to check the efficacy of remineralization. The result was compared with the artificial saliva group. Result: The CMC/LYZ-ACP nanogels were dense spherical particles with the particle size approximately 50-300 nm. SEM images showed that there were new crystals formed on the enamel surface after demineralization. Compared with the control group, the hardness and elastic modulus of the experimental group were closer to the normal enamel. Conclusion: CMC/LYZ-ACP nanogels are novel biomimetic materials that can be used for remineralization of demineralized enamel.

Key words: Enamel mineralized, Carboxymethyl chitosan, Lysozyme, Amorphous calcium phosphate