尼古丁促进大鼠结合上皮根向迁移的实验研究

doi:10.13701/j.cnki.kqyxyj.2021.10.006

口腔医学研究 ›› 2021, Vol. 37 ›› Issue (10): 890-893.DOI: 10.13701/j.cnki.kqyxyj.2021.10.006

尼古丁促进大鼠结合上皮根向迁移的实验研究

钟月月¹, 马肃^1,2*, 秦泽根¹, 周慧玲¹, 刘培红¹, 李延武¹

1.哈尔滨医科大学附属第一医院牙周科黑龙江哈尔滨 150001;
2.哈尔滨医科大学附属第一医院龙江学者实验室黑龙江哈尔滨 150001

收稿日期:2021-02-01 出版日期:2021-10-28 发布日期:2021-10-18
通讯作者: *马肃,E-mail:masu@yahoo.cn
作者简介:钟月月(1993~ ),女,陕西人,硕士在读,医师,研究方向:吸烟对结合上皮根向迁移的影响。
基金资助:
哈尔滨医科大学附属第一医院科研创新基金B(编号:2016B019)

Experimental Study on Nicotine Promoting Root Migration of Junctional Epithelium in Rats

ZHONG Yueyue¹, MA Su^1,2*, QIN Zegen¹, ZHOU Huiling¹, LIU Peihong¹, LI Yanwu¹

1. Department of Periodontics, First Affiliated Hospital of Harbin Medical University, Harbin 150001, China;
2. Longjiang Scholars Laboratory, First Affiliated Hospital of Harbin Medical University, Harbin 150001, China

Received:2021-02-01 Online:2021-10-28 Published:2021-10-18

摘要/Abstract

摘要： 目的:探讨尼古丁对大鼠结合上皮(junctional epithelium,JE)根向迁移的影响。方法:20只6周龄雄性Wistar大鼠随机等分为实验组(每日腹腔注射尼古丁0.73 mg/kg)和对照组(每日腹腔注射等量生理盐水),每组再等分为 30 d和90 d两个亚组;按实验设计处死后,常规制作右侧下颌磨牙区牙体-牙周联合组织的近远中向石蜡切片,Masson染色;测量第一磨牙釉牙骨质界-JE根端的长度。SPSS 26.0统计软件下各组数据行独立样本t检验。结果:30 d实验组和对照组相比,实验组大鼠JE出现轻度的根向迁移,但两组间无统计学意义(P＞0.05)。30 d组大鼠上皮下方的胶原均致密、粗大,排列整齐。对照、实验组90 d大鼠JE均有根向迁移,实验组迁移长度显著大于对照组(P＜0.01),实验组比对照组上皮下方的胶原稀疏,纤细。不同时间点间比较,实验组90 d大鼠JE根向迁移长度明显大于实验组30 d,两组间有显著统计学意义(P＜0.01)。结论:本研究表明,大鼠的JE以时间依赖的方式在顶端迁移,腹腔注射尼古丁可以增强这种迁移。

关键词: 尼古丁, 结合上皮, 胶原纤维, 根向迁移

Abstract: Objective: To investigate the effect of nicotine on root migration of junctional epithelium (JE) in rats. Methods: Twenty healthy male Wistar rats of six weeks of age were randomly divided into experimental group (intraperitoneal injection of nicotine 0.73mg/kg daily) and control group (intraperitoneal injection of the same amount of physiological saline daily). Each group was divided into two subgroups of 30 days and 90 days. After being killed according to the experimental design, the mesiodistal paraffin sections of tooth-periodontal joint tissue in the right mandibular molar area were routinely made and stained with Masson. The length of first molar enamel cementum boundary to the root apex of JE was measured. Results: On the 30th day, there was a slight root migration of JE in the experimental group compared with the control group without statistical significance (P>0.05). The collagen under the epithelium of both 30-day groups of rats was dense and neatly arranged. While the JE in the control and experimental groups had root migration at 90 days, the migration length of the experimental group was significantly longer than that of the control group (P<0.01), and the collagen under the epithelium of the experimental group was sparser and slender than the control group. In the different time points, the length of JE root migration in the experimental group at 90 days was significantly longer than that in the experimental group at 30 days (P<0.01). Conclusion: JE of rats migrated apically in a time-dependent manner, which could be enhanced by the intraperitoneal injection of nicotine.

Key words: nicotine, junctional epithelium, collagen fibers, root migration

钟月月, 马肃, 秦泽根, 周慧玲, 刘培红, 李延武. 尼古丁促进大鼠结合上皮根向迁移的实验研究[J]. 口腔医学研究, 2021, 37(10): 890-893.

ZHONG Yueyue, MA Su, QIN Zegen, ZHOU Huiling, LIU Peihong, LI Yanwu. Experimental Study on Nicotine Promoting Root Migration of Junctional Epithelium in Rats[J]. Journal of Oral Science Research, 2021, 37(10): 890-893.

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尼古丁促进大鼠结合上皮根向迁移的实验研究

Experimental Study on Nicotine Promoting Root Migration of Junctional Epithelium in Rats

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