口腔医学研究 ›› 2015, Vol. 31 ›› Issue (11): 1069-1072.

• 基金研究论著 • 上一篇    下一篇

牙齿煅烧颗粒结合富血小板纤维蛋白修复骨缺损的实验研究

郭津源,仲维剑*,柴松岭,梁欣,马国武   

  1. 大连医科大学口腔医学院口腔颌面外科 辽宁 大连 116044
  • 收稿日期:2015-07-06 出版日期:2015-11-28 发布日期:2016-03-21
  • 通讯作者: 仲维剑,Email:2061983zwj@163.com
  • 作者简介:郭津源(1988~),男,辽宁人,住院医师,硕士在读,主要从事口腔临床种植及基础研究工作。
  • 基金资助:
    辽宁省教育厅科学研究项目(编号:L2014359)

Bone Regeneration Effects of Tooth Ash in Conjunction with Platelet-rich Fibrin in an Animal Mode

GUO Jin-yuan, ZHONG Wei-jian, CHAI Song-ling, LIANG Xin, MA Guo-wu.   

  1. Stomatological College of Dalian Medical University, Dalian 116044, China
  • Received:2015-07-06 Online:2015-11-28 Published:2016-03-21

摘要: 目的:通过动物实验评价富血小板纤维蛋白(Platelet-Rich Fibrin,PRF)促进牙齿煅烧颗粒修复骨缺损的早期效果。方法:6月龄新西兰兔10只,于颅顶部制备4个骨缺损,每个骨缺损作为1个观察组。A组为空白对照,不填材料;B组为对照组,填入牙齿煅烧颗粒;C组为实验组,填入牙齿煅烧颗粒与PRF混合物;D组为阳性对照组,填入牙齿煅烧颗粒与骨髓混合物。术后6周取材,通过大体观察、X线检查和组织学分析骨缺损的修复效果。结果:各组骨缺损内均未见炎症反应,新骨生成率分别为:A组(7.5±2.2)%;B组(17.6±2.3)%;C组(21.0±4.7)%;D组(23.4%±3.2)%。统计学分析发现,B、C、D组的新骨生成率显著高于A组(P<0.05)。C组和D组的新骨生成率显著高于B组(P<0.05)。C组和D组间的新骨生成率没有显著性差异。结论:牙齿煅烧颗粒具有良好生物相容性和骨传导性,与PRF混合后可以显著加快新骨再生的速度,促进骨缺损的修复。

Abstract: Objective: To assess the initial effects of tooth ash in conjunction with platelet-rich fibrin (PRF) on reconstruction of bone defect. Methods: Ten white rabbits were selected to create an animal model with four defects on the skull. Defects in group A was used as vacant control, tooth ash particles were used solely in group B, tooth ash mixed with PRF were used in group C, and the defects in group D were filled with tooth ash combined with bone marrow. The animals were sacrificed 6 weeks later, bone samples were harvested for histological observation and statistical analysis. Results: The percentage of new bone formed in each group was 7.46%±2.21%, 17.61%±2.29%, 21.00%±4.73%, and 23.42%±3.22%, respectively. Group C and D showed a statistically greater amount of new bone formation compared with group A and B (P<0.05). No significant differences were found between group C and D. Conclusion: Teeth ash particle is a type of bone graft material with favorable biocompatibility and osteoconductivity. After in conjunction with PRF, it can facilitate the bone healing process in early stage.

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