口腔医学研究

口腔医学研究 ›› 2015, Vol. 31 ›› Issue (11): 1064-1068.

• 基金研究论著 • 上一篇    下一篇

复方黄芩片对人牙周膜细胞及牙周炎牙槽骨OPG/RANKL表达的影响

张江琳,王浩宇#,李丹,杨相笛,石磊,罗宁,陈悦*   

  1. 西安交通大学口腔医学院牙周黏膜科 陕西 西安 710004
  • 收稿日期:2015-06-16 出版日期:2015-11-28 发布日期:2016-03-21
  • 通讯作者: 陈悦,电话:13572892935
  • 作者简介:张江琳(1989~),女,山东人,硕士在读,主要从事牙周病学研究。

Effects of Compound Skullcap Tablets on the Expression of OPG/RANKL in Human Periodontal Ligament Cells and Alveolar with Periodontitis.

ZHANG Jiang-lin, WANG Hao-yu, LI Dan, YANG Xiang-di, SHI Lei, LUO Ning, CHEN Yue.   

  1. Department of Periodontal and Oral Mucosal Diasease, Stomatology Hospital of Xi'an Jiaotong University College of Medicine, Xi'an 710004, China
  • Received:2015-06-16 Online:2015-11-28 Published:2016-03-21

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摘要： 目的:探讨复方黄芩片对人牙周膜细胞表面以及对实验性牙周炎大鼠牙槽骨OPG/RANKL表达的影响。方法:采用酶消化组织块法培养hPDLCs,通过ELISA法测定复方黄芩片对LPS诱导下hPDLCs表面OPG/RANKL表达的影响;LPS局部注射法建立大鼠牙周炎模型,免疫组化法测定复方黄芩片对牙周炎大鼠牙槽骨OPG/RANKL表达的影响。结果:0.2 mg/L复方黄芩片溶液对10 mg/L LPS诱导下的hPDLCs表面OPG表达无明显影响,而hPDLCs表面RANKL的表达显著降低(P<0.05),且实验组 hPDLCs表面OPG/RANKL的比值较空白对照组显著降低(P<0.05);复方黄芩片治疗3周后各组大鼠OPG/RANKL免疫组化染色阳性细胞计数结果显示牙周炎组大鼠牙周组织中RANKL的表达水平明显高于其他各组,治疗组OPG表达水平明显高于其他各组。结论:复方黄芩片可以上调牙周膜细胞表面OPG/RANKL的比值;按黄芩苷2.85 g/kg·d-1的剂量给予牙周炎大鼠复方黄芩片灌胃治疗,复方黄芩片可以抑制牙周炎牙槽骨中RANKL的表达,促进OPG的表达。提示复方黄芩片有望用于牙周炎的临床治疗。

Abstract: Objective: To investigate effects of compound skullcap tablets on the expression of OPG/RANKL in human periodontal ligament cells (hPDLCs) and alveolar with periodontitis in SD rats. Methods: hPDLCs were isolated and identified in vitro. ELISA was used to detect the effects of compound skullcap tablets on the expression of OPG/RANKL. Rat periodontitis model was constructed by injecting LPS locally, and immunohistochemistry was used to detect effects of compound skullcap tablets on the expression of OPG/RANKL of alveolar bone in vivo. Results: Compound skullcap tablets at the concentration of 0.2μg/ml could significantly reduce the expression of RANKL in hPDLCs in vitro (P<0.05), while the expression of OPG was not significantly changed, which led to an increased ratio of OPG/RANKL (P<0.05). Immunohistochemistry showed the highest expression level of OPG in treatment group (P<0.05) and the highest expression of RANKL in periodontitis rats. Conclusion: Compound skullcap tablets can increase the ratio of OPG/RANKL and may be used to treat periodontitis clinically in the future.

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