口腔微生物与不良妊娠结局关系的研究进展

doi:10.13701/j.cnki.kqyxyj.2017.08.026

口腔医学研究 ›› 2017, Vol. 33 ›› Issue (8): 905-908.DOI: 10.13701/j.cnki.kqyxyj.2017.08.026

• 综述 • 上一篇

口腔微生物与不良妊娠结局关系的研究进展

陈智慧¹, 吕莉娟², 张亮³, 叶宁^1*

1. 广州医科大学附属广东省妇儿医院口腔医学中心广东广州 511400;
2. 广州医科大学附属广东省妇儿医院产科广东广州 511400;
3. 广州医科大学附属广东省妇儿医院转化医学中心广东广州 511400

收稿日期:2016-06-11 出版日期:2017-08-20 发布日期:2017-08-28
作者简介:陈智慧(1990~ ),女,广东人,硕士在读,主要从事孕妇牙周疾病的研究工作。
基金资助:
广东省医学科研基金(编号:B2014025)

Research Progress of Relationship Between Oral Organisms and Adverse Pregnancy Outcomes.

CHEN Zhi-hui¹, LV Li-juan², ZHANG Liang³, YE Ning^1*

1. Department of Stomatology, Guangdong Women and Children Hospital of Guangzhou Medical University, Guangzhou 511400, China;
2. Department of Obstetrics, Guangdong Women and Children Hospital, Guangzhou 511400, China;
3. Department of Translational Medicine Research Center, Guangdong Women and Children Hospital, Guangzhou 511400, China.

Received:2016-06-11 Online:2017-08-20 Published:2017-08-28

摘要/Abstract

摘要： 长久以来,胎盘一直被认为是无菌环境,但最新研究采用分子生物学技术,发现胎盘内同样存在其独特的微生物群落,且与口腔微生物群落最为接近。经血行途径散播的口腔致病菌已成为宫内感染的另一重要来源。本文就近年来国内外对口腔微生物入侵子宫的研究及其对不良妊娠结局(adverse pregnancy outcomes,APOs)的影响进行探讨,为孕妇口腔疾病的预防和临床治疗提供新的方向。

关键词: 口腔微生物, 胎盘微生物, 不良妊娠结局, 16S, rRNA基因

Abstract: The placentas of healthy pregnancies were always considered to be bacteria-free environment. But novel findings characterized a unique microbiome niche in the placenta with molecular approaches, which may be most akin to the human oral microbiome. The increased oral bacteria detection in placental tissue, suggesting a hematogenous spread of bacteria that originates from oral microflora may be associated with intrauterine infection.The article aims to review current researches about the microbe from oral cavity invade the placenta and its potential relationship with adverse pregnancy outcomes (APOs).

Key words: Oral microbiome, Placetal microbiome , Adverse pregnancy outcomes 16S , rRNA gene

中图分类号:

R780.1

陈智慧, 吕莉娟, 张亮, 叶宁. 口腔微生物与不良妊娠结局关系的研究进展[J]. 口腔医学研究, 2017, 33(8): 905-908.

CHEN Zhi-hui, LV Li-juan, ZHANG Liang, YE Ning. Research Progress of Relationship Between Oral Organisms and Adverse Pregnancy Outcomes.[J]. Journal of Oral Science Research, 2017, 33(8): 905-908.

参考文献

[1] Aagaard K, Ma J, Antony K M, et al. The placenta harbors a unique microbiome [J]. Science Translational Medicine, 2014, 6(237)∶237ra65-237ra65
[2] Wei Z, Tsompana M, Ruscitto A, et al. An accurate and efficient experimental approach for characterization of the complex oral microbiota [J]. Microbiome, 2014, 3(1)∶1-11
[3] 高岚,熊浩山,彭莉,等.以16S rRNA为基础的检测技术在肠道菌群研究中的应用[J].中国畜牧杂志,2008,44(15)∶56-60
[4] 车宇薇,王燕一.宏基因组学在口腔微生物研究中的应用进展[J].中华老年口腔医学杂志,2015,13(4)∶249-252
[5] Digiulio DB. Diversity of microbes in amniotic fluid [J]. Seminars in Fetal & Neonatal Medicine, 2012, 17(1)∶2-11
[6] Funkhouser LJ, Bordenstein SR. Mom knows best: the universality of maternal microbial transmission [J]. Plos Biology, 2013, 11(8)∶318-329
[7] Stout MJ, Conlon B, Landeau M, et al. Identification of intracellular bacteria in the basal plate of the human placenta in term and preterm gestations [J]. American Journal of Obstetrics & Gynecology, 2013, 208(3)∶1-7
[8] Dethlefsen L, Mcfall-Ngai M, Relman D A. An ecological and evolutionary perspective on human-microbe mutualism and disease [J]. Nature, 2007, 449(7164)∶811-8
[9] Prince AL, Ma J, Kannan PS, et al. The Placental Microbiome is Altered Among Subjects with Spontaneous Preterm Birth with and without Chorioamnionitis [J]. American Journal of Obstetrics and Gynecology, 2016, 214(5)∶627.e1-627.e16
[10] Fardini Y, Chung P, Dumm R, et al. Transmission of diverse oral bacteria to murine placenta: evidence for the oral microbiome as a potential source of intrauterine infection [J]. Infection & Immunity, 2010, 78(4)∶1789-96
[11] Han Y W. Oral health and adverse pregnancy outcomes - what's next [J]. Journal of Dental Research, 2010, 90(3)∶289-293
[12] 时春艳,樊尚荣.羊膜腔感染的诊断和处理[J].中华产科急救电子杂志,2013,2(2)∶33-36
[13] Han Y W. Fusobacterium nucleatum: a commensal-turned pathogen [J]. Current Opinion in Microbiology, 2015, 23∶141-147
[14] Yann Fardini, Xiaowei Wang, Témoin S, et al. Fusobacterium nucleatum, adhesin FadA binds vascular endothelial cadherin and alters endothelial integrity [J]. Africa, 2011, 82(6)∶1468-1480
[15] Ikegami A, Chung P, Han YW. Complementation of the fadA mutation in Fusobacterium nucleatum demonstrates that the surface-exposed adhesin promotes cellular invasion and placental colonization [J]. Infection and Immunity,2009,77(7)∶3075-3079
[16] Gauthier S, Tetu A, Himaya E, et al. The origin of Fusobacterium nucleatum involved in intra-amniotic infection and preterm birth [J]. J Matern Fetal Neonatal Med, 2011, 24(11)∶1329-1332
[17] Saito A, Inagaki S, Kimizuka R, et al. Fusobacterium nucleatum, enhances invasion of human gingival epithelial and aortic endothelial cells by Porphyromonas gingivalis [J]. Fems Immunology & Medical Microbiology, 2008, 54(3)∶349-355
[18] James J L, Stone P R, Chamley L W. The regulation of trophoblast differentiation by oxygen in the first trimester of pregnancy [J]. Human Reproduction Update, 1990, 43(1)∶124-6
[19] Katz J, Chegini N, Shiverick KT, et al. Localization of P. gingivalis in preterm delivery placenta [J]. Journal of Dental Research, 2009, 88(6)∶575-8
[20] León R, Silva N, Ovalle A, et al. Detection of Porphyromonas gingivalis in the amniotic fluid in pregnant women with a diagnosis of threatened premature labor [J]. Journal of Periodontology, 2007, 78(7)∶1249-55
[21] Yiping W. Han, Akihiko Ikegami, Nabil F. Bissada, Melissa Herbst, Raymond W, et al. Transmission of an Uncultivated Bergeyella Strain from the Oral Cavity to Amniotic Fluid in a Case of Preterm Birth [J]. Journal of Clinical Microbiology, 2006, 44(4)∶1475-83
[22] Madianos PN, Bobetsis YA, Offenbacher S. Adverse pregnancy outcomes (APOs) and periodontal disease: pathogenic mechanisms [J]. Journal of Periodontology, 2013, 84(4 Suppl)∶170-80
[23] Digiulio DB, Romero R, Kusanovic JP, et al. Prevalence and Diversity of Microbes in the Amniotic Fluid, the Fetal Inflammatory Response, and Pregnancy Outcome in Women with Preterm Pre-Labor Rupture of Membranes [J]. American Journal of Reproductive Immunology, 2010, 64(1)∶38-57
[24] Stockham S, Stamford JE, Roberts CT, et al. Abnormal pregnancy outcomes in mice using an induced periodontitis model and the haematogenous migration of Fusobacterium nucleatum sub-species to the murine placenta [J]. Plos One, 2015, 10(3)∶
[25] Bohrer JC, Kamemoto LE, Almeida PG, et al. Acute chorioamnionitis at term caused by the oral pathogen Fusobacterium nucleatum [J]. Hawaii Journal of Medicine & Public Health A Journal of Asia Pacific Medicine & Public Health, 2012, 71(10)∶280-1
[26] Barak S, Oettinger-Barak O, Machtei EE, et al. Evidence of periopathogenic microorganisms in placentas of women with preeclampsia [J]. Journal of Periodontology, 2007, 78(4)∶670-6
[27] Gonzalesmarin C, Spratt DA, Allaker RP. Maternal oral origin of Fusobacterium nucleatum in adverse pregnancy outcomes as determined using the 16S-23S rRNA gene intergenic transcribed spacer region [J]. Journal of Medical Microbiology, 2013, 62(1)∶133-44
[28] Han YW, Fardini Y, Chen C, et al. Term stillbirth caused by oral Fusobacterium nucleatum [J]. Obstetrics & Gynecology, 2010, 115(2 Pt 2)∶442-445
[29] Wang X, Buhimschi CS, Temoin S, et al. Comparative Microbial Analysis of Paired Amniotic Fluid and Cord Blood from Pregnancies Complicated by Preterm Birth and Early-Onset Neonatal Sepsis [J]. Plos One, 2013, 8(2)∶e56131
[30] Collado MC, Rautava S, Aakko J, et al. Human gut colonisation may be initiated in utero by distinct microbial communities in the placenta and amniotic fluid [J]. Scientific Reports, 2016, 6∶p. 23129

口腔微生物与不良妊娠结局关系的研究进展

Research Progress of Relationship Between Oral Organisms and Adverse Pregnancy Outcomes.

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