16S rDNA测序分析微酸性电解水对牙周炎患者口腔菌群的影响

doi:10.13701/j.cnki.kqyxyj.2020.11.007

口腔医学研究 ›› 2020, Vol. 36 ›› Issue (11): 1012-1015.DOI: 10.13701/j.cnki.kqyxyj.2020.11.007

16S rDNA测序分析微酸性电解水对牙周炎患者口腔菌群的影响

曹程¹, 李平², 胡贞卿³, 李自良^3*

1.昆明医科大学附属口腔医院前兴路门诊部云南昆明 650000;
2.昆明市中医医院云南昆明 650000;
3.昆明医科大学云南昆明 650000

收稿日期:2020-05-14 出版日期:2020-11-28 发布日期:2020-11-27
通讯作者: *李自良,E-mail:1752114604@qq.com
作者简介:曹程(1990~ ),男,云南昆明人,硕士,医师,研究方向:口腔医学。
基金资助:
云南省卫生和计划生育委员会医学后备人才项目(H-2017054)云南省应用基础研究(昆医联合专项)项目[2017FE467(-085)]

Effect of Slightly Acidic Electrolyzed Water on Oral Flora in Patients with Periodontitis by 16S rDNA Sequencing Analysis

CAO Cheng¹, LI Ping², HU Zhenqing³, LI Ziliang^3*

1. Qianxing Road Clinic, Affiliated Stomatological Hospital of Kunming Medical University,Kunming 650000, China;
2. Kunming Traditional Chinese Medicine Hospital, Kunming 650000, China;
3. Kunming Medical University, Kunming 650000, China

Received:2020-05-14 Online:2020-11-28 Published:2020-11-27

摘要/Abstract

摘要： 目的:探讨微酸性电解水含漱液对牙周炎患者口腔菌群的影响。方法:选择10例进入支持治疗的牙周炎患者,收集其使用微酸性电解水含漱7 d前、后的唾液样本,通过16S rDNA测序法对样本进行检测,分析口腔菌群的变化。结果:用微酸性电解水含漱7 d后,门水平,拟杆菌门、梭菌门、变形菌门物种相对丰度降低。属水平,奈瑟菌属、梭杆菌属、卟啉单胞菌属、普雷氏菌属物种相对丰度降低。α多样性分析:Simpson指数增加,Shannon指数增加(P<0.05),Chao1指数、ACE指数、PD_whole_tree指数均升高(P<0.01)。β多样性指数分析显示组间差异性显著(P<0.01)。结论:含漱微酸性电解水可以改变口腔菌群的结构,增加菌群种类及多样性。

关键词: 微酸性电解水, 16S rDNA, 口腔菌群, 牙周炎

Abstract: Objective: To investigate the effect of slightly acidic electrolyzed water (SAEW) gargle on oral flora in patients with periodontitis. Methods: The saliva samples gargled with slightly acidic electrolyzed water of 10 patients with periodontitis who entered the supportive periodontal therapy were collected before and after 7 days. The total DNA of oral flora was extracted and sequenced by 16S rDNA sequencing, and the changes of oral flora diversity and structure were analyzed. Results: After gargled with slightly acidic electrolyzed water for 7 days, at phylum level, the relative abundance of proteobacteria, bacteroidetes and fusobacteria species was decreased. At the genus level, the relative abundance of periodontal pathogen genera-neisseria, porphyromonas, clostridium, and pretenella was decreased. Analysis of α-diversity: the Simpson index and Shannon index increased (P<0.05), Chao1 index, ACE index, and PD_whole_tree index were all increased (P<0.01). The analysis of the β-diversity index showed significant differences between the groups (P<0.01). Conclusion: The use of slightly acidic electrolyzed water can change the structure of oral flora and increase the abundance and diversity of oral flora in patients with periodontitis, which is conducive to the restoration of oral flora to the normal range.

Key words: slightly acidic electrolyzed water, 16S rDNA, oral flora, periodontitis

曹程, 李平, 胡贞卿, 李自良. 16S rDNA测序分析微酸性电解水对牙周炎患者口腔菌群的影响[J]. 口腔医学研究, 2020, 36(11): 1012-1015.

CAO Cheng, LI Ping, HU Zhenqing, LI Ziliang. Effect of Slightly Acidic Electrolyzed Water on Oral Flora in Patients with Periodontitis by 16S rDNA Sequencing Analysis[J]. Journal of Oral Science Research, 2020, 36(11): 1012-1015.

参考文献

[1] 束蓉,倪靖.2018牙周病和植体周病国际新分类——牙周炎分期分级疾病定义系统临床应用体会[J/OL].口腔医学,2020(01):1-6.
[2] Chen C, Hemme C, Beleno J, et al. Oral microbiota of periodontal health and disease and their changes after nonsurgical periodontal therapy [J]. The ISME Journal, 2018, 12(5).
[3] Hajishengallis, George. Dysbiosis and inflammation in periodontitis: synergism and implications for treatment [J]. Journal of Oral Microbiology, 2017, 9(sup1):1325198.
[4] Supranoto S, Slot D, Addy M, et al. The effect of chlorhexidine dentifrice or gel versus chlorhexidine mouthwash on plaque, gingivitis, bleeding and tooth discoloration: a systematic review [J]. International Journal of Dental Hygiene, 2015, 13(2):83-92.
[5] Coelho AS, Laranjo M, Gonçalves AC, et al. Cytotoxic effects of a chlorhexidine mouthwash and of an enzymatic mouthwash on human gingival fibroblasts [J]. Odontology 108, 260–270 (2020).
[6] Kim Hyun-Ji, Tango Charles Nkufi, Chelliah Ramachandran, et al. Sanitization Efficacy of Slightly Acidic Electrolyzed Water against pure cultures of Escherichia coli, Salmonella enterica, Typhimurium, Staphylococcus aureus and Bacillus cereus spores, in Comparison with Different Water Hardness [J]. Scientific reports,2019,9(1).
[7] You HS, Fadriquela A, Sajo MEJ, et al. Wound Healing Effect of Slightly Acidic Electrolyzed Water on Cutaneous Wounds in Hairless Mice via Immune-Redox Modulation [J]. Biological & Pharmaceutical Bulletin, 2017, 40(9):1423-1431.
[8] 植原,治,舞田,健夫,齊藤,正人.顔面皮膚および口腔粘膜に対する微酸性電解水の消毒効果[J].日本口腔検査学会雑誌,2014,6:38－43.
[9] Kashiwabara T, Y oneyama T, Nakamichi A, et al. In vitroefficacy of electrolyzed dilute sodium hypochlorite solution on candidal biofilm [J]. Ronen Shika Igaku, 2014, 28:277-283.
[10] 徐静,熊纪敏,辛鹏举,等.微酸性电解水的性能与口腔应用研究[J].中华医院感染学杂志,2018,28(12):1913-1916.
[11] Hall MW, Singh N, Ng KF, et al. Inter-personal diversity and temporal dynamics of dental, tongue, and salivary microbiota in the healthy oral cavity [J]. npj Biofilms and Microbiomes, 2017, 3(1):2.
[12] 周学东,徐健,施文元.人类口腔微生物组学研究:现状、挑战及机遇[J].微生物学报,2017,57(06):806-821+792.
[13] 熊雄,易书瀚,赵紫薇,等.基于高通量测序分析健康人咀嚼槟榔前后口腔菌群多样性[J].中国微生态学杂志,2018,30(04):419-423.
[14] Shaikh H, Patil SH, Pangam TS. Polymicrobial synergy and dysbiosis: an overview [J]. J Indian Soc Periodontol, 2018, 22(2): 101-106.
[15] 葛林虎,苏汉福.口腔菌群多样性与菌株基因组多态性[J].口腔医学研究,2019,35(10):915-918.
[16] Lakshman, Samaranayake, Victor, et al. Normal Oral Flora and the Oral Ecosystem [J]. Dental Clinics of North America, 2017.
[17] Karpiński TM. Role of oral microbiota in cancer development [J]. Microorganisms, 2019, 7(1): E20.
[18] Dominy SS, Lynch C, ErminiF, et al. Porphyromonas gingivalis in Alzheimer's disease brains:Evidence for disease causation and treatment with small-molecule inhibitors [J]. Sci Adv,2019,5(1):eaau3333.
[19] Perricone C, Ceccarelli F, MatteoS, et al. Porphyromonas gingivalis and rheumatoid arthritis [J]. CurrOpin Rheumatol, 2019,31(5):517-524.
[20] Alhathloul, S. and Ali, A. (2020) Effect of Using Mouthwash Solution on Commensal Flora of the Oral Cavity among Female Students in Qassim University [J]. Journal of Biosciences and Medicines, 8, 135-142.

16S rDNA测序分析微酸性电解水对牙周炎患者口腔菌群的影响

Effect of Slightly Acidic Electrolyzed Water on Oral Flora in Patients with Periodontitis by 16S rDNA Sequencing Analysis

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