中度和重度牙周炎患者龈下细菌多样性和群落结构分析

doi:10.13701/j.cnki.kqyxyj.2018.08.012

摘要/Abstract

摘要： 目的:通过16S rDNA测序探索和比较重度牙周炎和中度牙周炎患者龈下细菌多样性及群落结构。方法:选取17例中度或重度牙周炎患者作为研究对象,根据症状将其分为重度牙周炎组9例与中度牙周炎组8例。收集提取其牙周袋内龈下细菌进行16S rDNA测序,然后对每个样品的可操作分类单元(operational taxonomy unit,OTU)丰度和每个水平的分类(域、界、门、纲、目、科、属、种)进行统计并进行α多样性与β多样性分析,最后对每个样品的16S rDNA组成数据分析并预测细菌功能。结果:从龈下样本中获得的高质量的序列(555028)分为373个OTUs,代表303个独立的种,隶属于138属75科,47目,28类,12门。两组共332个OTUs,表明核心龈下微生物的存在。龈下微生物的优势菌门包括拟杆菌门、厚壁菌门、梭杆菌门、变形菌门和螺旋菌门,优势菌属包括梭形杆菌属、链球菌属、卟啉单胞菌属、普氏菌属、奈瑟氏菌属和密螺旋体属。α多样性分析结果表明,重度牙周炎患者的微生物多样性高于中度牙周炎组。β多样性分析表明两组微生物群落结构相似。所有样品中共预测到39种代谢功能,主要集中于膜转运(10.9%)、复制和修复(9.9%)、糖代谢(9.3%)、氨基酸代谢(9.1%)、翻译(6.9%)、能量代谢(5.8%)和不良性征(5.1%)。结论:牙周炎患者龈下细菌具有丰富的多样性与稳定的群落结构,龈下细菌的功能与口腔其他部位的细菌相同。牙周炎疾病的严重程度与龈下细菌的多样性有关,但与细菌的群落结构尚未发现相关性。鉴于牙周炎的微生物多样性和共生性,调控微生物相互作用及其功能发挥的治疗策略应得到进一步发展。

关键词: 牙周炎, 龈下, 细菌多样性, 16S rDNA测序

Abstract: Objectve: To investigate and compare the oral microbial community from patients suffering severe and mild periodontitis by using high throughput sequencing of 16s rDNA genes. Methods: Seventy patients with moderate or severe periodontitis were selected as the subjects. According to their symptoms, they were divided into SP (severe periodontitis, 9 cases) group and MP (moderate periodontitis, 8 cases) group. The subgingival bacteria of the patients were sequenced with 16s rDNA. Then the OUT(operational taxonomic units)abundance of each sample and the classification of each level (domain, boundary, gate, class, order, family, genus, and species) were counted and analyzed with α and β diversity. Finally, the data of 16S rRNA for each sample were analyzed and the function of bacteria was predicted. Results: The high quality sequence (555028) obtained from subgingival samples (555028) was divided into OTUs, representing 303 independent species, belonging to 138 genera, 75 families, 47 orders, 28 classes and 12 phyla. Two groups shared 332 OTUs, indicating the presence of a core subgingival microbiome. α diversity analysis showed that the microbial diversity in severe periodontitis exceeded that of moderate periodontitis. The dominant phyla of subgingival microbiota included Bacteroidetes, Firmicutes, Fusobacteria, Proteobacteria, and Spirochaetae. The dominant genera included Fusobacterium, Streptococcus, Porphyromonas, Prevotella, Neisseria, and Treponema. β diversity analysis showed that the microbial community structure was similar in two groups. All the samples were predicted 39 kinds of metabolic function, mainly concentrated in the membrane transport (10.9%),replication and repair (9.9%), glucose metabolism (9.3%), amino acid metabolism (9.1%), translation (6.9%), energy metabolism (5.8%), and bad character (5.1%). Conclusion: The subgingival bacteria of periodontitis have rich diversity and stable community structure. The function of subgingival bacteria is the same as the bacteria in other parts of the mouth. The severity of periodontitis is related to the diversity of subgingival bacteria, but not related to the community structure of the bacteria. In view of the microbial diversity and symbiosis of periodontitis, the therapeutic strategies for regulating the interaction and function of microorganism should be further developed.

Key words: Periodontitis, Subgingiva, Bacterial diversity, 16S rDNA sequencing

范华南, 黄辉, 姜庆坤, 刘漪. 中度和重度牙周炎患者龈下细菌多样性和群落结构分析[J]. 口腔医学研究, 2018, 34(8): 846-851.

FAN Hua-nan, HUANG Hui, JIANG Qing-kun, LIU Yi. Diversity and Community Structure of Subgingival Bacterial from Patients with Moderate or Severe Periodontitis[J]. Journal of Oral Science Research, 2018, 34(8): 846-851.

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