口腔医学研究

口腔医学研究 ›› 2017, Vol. 33 ›› Issue (10): 1048-1051.DOI: 10.13701/j.cnki.kqyxyj.2017.10.007

• 基础研究论著 • 上一篇    下一篇

傅里叶红外变换光谱(FT-IR)技术用于口腔微生物的初步鉴别

蔡叶，杨卫东*   

  1. 南京大学医学院附属口腔医院牙体牙髓科 江苏 南京 210000
  • 收稿日期:2017-04-25 出版日期:2017-10-20 发布日期:2017-10-24
  • 通讯作者: 杨卫东,E-mail:ywdong2001@163.com
  • 作者简介:蔡叶(1984~ ),男,江苏人,硕士,主治医师,主要从事牙体牙髓科的临床治疗工作。
  • 基金资助:
    江苏省重点研发计划(社会发展)项目(编号:BE2016623)

Preliminary Identification of Oral Microorganisms by Fourier Transform Infrared Spectroscopy (FT-IR)

CAI Ye, YANG Wei-dong*   

  1. Nanjing Stomatological Hospital of Nanjing University Medical School, Nanjing University, Nanjing 210000, China.
  • Received:2017-04-25 Online:2017-10-20 Published:2017-10-24

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摘要： 目的:应用傅里叶红外光谱技术(FT-IR)对健康青年口腔微生物进行初步的鉴别。方法:用傅里叶红外光谱对30个健康青年口腔分离提取到的50株典型菌株进行指纹图谱数据采集,采用求平均、一阶导数、矢量归一化处理图谱后进行聚类分析与标准菌株进行比较。结果:在特征谱区:3000～2800,1500～1400,1200～900 cm-1下,分离菌株与各标准菌株相比得到很好地归类。结论:傅里叶红外变换光谱具有准确,快速,方便,时效等优点,可以为口腔微生物的初步鉴别提供一个可靠地的方法,对临床许多可能由微生物引发的感染性疾病具有指导意义,以便建立口腔微生物群落疾病预警系统。

关键词: 傅里叶红外变换光谱, 口腔微生物

Abstract: Objective: To identify oral microorganisms extracted from healthy young people by Fourier transform infrared spectroscopy (FT-IR). Methods: FT-IR fingerprint data of 50 typical strains, which were extracted from oral cavity of 30 healthy people, were analyzed by a series of methods like averaging, the first derivative, and vector normalization. They were analyzed by the cluster methods and compared with the standard strains. Results: Isolated strains were easily to be compared with the standard strains in the characteristic spectrum area at 3000-2800, 1500-1400, and 1200-900cm-1. Conclusion: As a technique which was accurate, fast, convenient, and time-effect, FT-IR was a reliable method for preliminary evaluation of oral microorganisms. It has guiding significance in establishing an early warning system for diseases caused by oral microorganisms.

Key words: FT-IR , Oral microorganisms

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