口腔医学研究

口腔医学研究 ›› 2018, Vol. 34 ›› Issue (2): 169-171.DOI: 10.13701/j.cnki.kqyxyj.2018.02.015

• 口腔材料学研究 • 上一篇    下一篇

不同锌含量Zn/PLGA/HA复合支架对人牙髓干细胞粘附能力影响的体外研究

刘旭妍, 吴兆玉, 王冉旭, 何丽娜, 潘爽, 牛玉梅*   

  1. 哈尔滨医科大学口腔医学院牙体牙髓病科 黑龙江 哈尔滨 150001;
  • 收稿日期:2017-11-09 出版日期:2018-02-28 发布日期:2018-02-26
  • 通讯作者: 牛玉梅,E-mail:yumeiniu@163.com
  • 作者简介:刘旭妍(1992~ ),女,黑龙江北安人,硕士,医师,主要从事牙体牙髓病科临床治疗工作。
  • 基金资助:
    国家自然科学基金资助项目(编号:81570963)

Adhesion of Dental Pulp Stem Cells on Zn/polylactic-co-glycolic acid/hydroxylapatite Composite Scaffolds with Different Zinc Contents in Vitro

LIU Xu-yan, WU Zhao-yu, WANG Ran-xu, HE Li-na, PAN Shuang, NIU Yu- mei*   

  1. Department of Endodontics, College of Stomatology, Harbin Medical University, Harbin 150001, China.
  • Received:2017-11-09 Online:2018-02-28 Published:2018-02-26

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摘要： 目的:制备含有不同锌含量的Zn/PLGA/HA复合支架并检测其机械性能;探讨不同锌含量的Zn/PLGA/HA复合支架在体外对人牙髓干细胞粘附能力的影响。方法:通过溶液浇铸/粒子沥滤法制备含锌质量分数0.5%、1%及2%的Zn/PLGA/HA复合支架,每组HA含量均为20%质量分数,对照组为不含锌的20%HA/PLGA支架。使用扫描电镜观察支架的表面形态,电子万能试验机检测支架的拉伸强度。将人牙髓干细胞接种在不同锌含量的Zn/PLGA/HA复合支架上培养,采用DAPI染色细胞计数法检测细胞粘附能力。结果:含锌质量分数1%和2%的Zn/PLGA/HA复合支架组拉伸强度均高于对照组(P<0.05)。扫描电镜观察到各组支架表面孔隙率均较高(87.6%),孔径158～372 μm,并具有良好的孔间连通性。0.5%Zn组支架与对照组相比具有较好的促牙髓干细胞粘附能力(P<0.05)。结论:本实验中,含1%,2% Zn(wt%)的Zn/PLGA/HA支架具有较好的机械性能,含0.5%Zn(wt%)的Zn/PLGA/HA支架在体外对人牙髓干细胞粘附具有更好的促进作用,为含锌支架作为支架材料用于组织工程提供了理论基础。

关键词: 锌, PLGA, HA, 人牙髓干细胞, 组织工程

Abstract: Objective: To prepare Zn/polylactic-co-glycolic acid/hydroxylapatite(Zn/PLGA/HA) composite scaffolds with different zinc contents and evaluate the adhesion of dental pulp stem cells on Zn/PLGA/HA composite scaffolds. Methods: Zn/PLGA/HA composite scaffolds with different zinc contents (0.5, 1 and 2wt%) and HA(20wt%) content were fabricated by solvent casting and particulate leaching technology.The control group was fabricated with PLGA and 20% HA without Zinc.The surface structure of the scaffolds was observed by scanning electron microscopy (SEM).The tensile strength of the scaffolds was detected by an electro-mechanical universal testing machine.Human dental pulp stem cells were cultured with Zn/PLGA/HA composite scaffolds with different zinc contents.The cell adhesion ability was measured by DAPI staining. Results: The tensile strengths of 1% and 2% Zn groups were higher than that of control group(P<0.05). The scaffolds showed high porosity (87.6%) and the pores were highly interconnected.The pore size was 158~372 μm. The cell adhesion ability in 0.5% Zn group was better than the control group (P<0.05). Conclusion: One and two percent Zn groups show a higher tensile strength, while the adhesion ability of hDPSCs in 0.5% Zn group is better in vitro, which provides a theoretical basis for zinc-containing scaffold in tissue engineering.

Key words: Zinc, PLGA, HA, Human dental pulp stem cells, Tissue engineering

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