口腔医学研究

口腔医学研究 ›› 2020, Vol. 36 ›› Issue (9): 871-874.DOI: 10.13701/j.cnki.kqyxyj.2020.09.016

• 口腔抗菌材料研究 • 上一篇    下一篇

二氧化钛纳米管/抗菌肽控释系统的构建及初步性能研究

苗强1,2, 郑亚飞1, 夏雨凝1, 张薇1, 王菁1, 马赛1*, 马楚凡1*   

  1. 1.军事口腔医学国家重点实验室,口腔疾病国家临床医学研究中心,陕西省口腔医学重点实验室,空军军医大学第四军医大学口腔医院修复科 陕西 西安 710032;
    2.解放军总医院第六医学中心口腔科 北京 100048
  • 收稿日期:2019-12-20 出版日期:2020-09-28 发布日期:2020-09-15
  • 通讯作者: *马赛,E-mail: drmasai@fmmu.edu.cn;马楚凡,E-mail: machufan@fmmu.edu.cn
  • 作者简介:苗强(1992~),男,北京人,医师,硕士,研究方向:口腔种植材料,现工作于解放军总医院第六医学中心口腔科。
  • 基金资助:
    国家自然科学基金(编号:31470929)

Construction and Characterization of TiO2 Nanotube Coating with Controlled Releasing Antibacterial Peptide

MIAO Qiang1,2, ZHENG Yafei1, XIA Yuning1, ZHANG Wei1, WANG Jing1, MA Sai1*, MA Chufan1*   

  1. 1. State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shanxi Key Laboratory of Stomatology, Department of Prosthodontics, School of Stomatology, The Fourth Military Medical University, Xi'an 710032, China;
    2. Department of Stomatology, The Sixth Medical Center, PLA General Hospital, Beijing 100048, China
  • Received:2019-12-20 Online:2020-09-28 Published:2020-09-15

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摘要： 目的: 为了解决经皮种植体的高感染率问题,在纯钛表面构建二氧化钛纳米管涂层并通过聚乳酸羟基乙酸共聚物(poly lactic-co-glycolic acid, PLGA)微球复合抗菌肽HHC-36实现抗菌药物的加载。对实验材料抗菌肽缓释性能及抗菌性能进行初步探讨。方法: 阳极氧化法在纯钛表面构建二氧化钛纳米管涂层,随后以PLGA做为抗菌肽的缓释剂,采用双重溶剂乳化挥发法在二氧化钛纳米管表面加载抗菌物质。扫描电镜观察试件微观形貌,测定抗菌肽缓释曲线,抑菌环法检测实验材料的抗菌性能。结果: 扫描电镜下可见纯钛试件表面形成排列整齐的、管径为(100±20) nm的二氧化钛纳米管涂层。加载PLGA微球后可见有空隙的微球结构。载药试件可实现15 d以上的抗菌肽持续、稳定释放。抑菌实验证实,载药试件可形成直径14 mm的抑菌环,且该抑菌环在10 d内无显著变化。结论: 本实验成功在纯钛表面构建了二氧化钛纳米管复合抗菌肽缓释微球的纳米抗菌涂层,该载药涂层表现出良好的抗菌性能。

关键词: TiO2纳米管, PLGA缓释微球, 抗菌涂层, 抗菌肽HHC-36

Abstract: Objective: To construct a TiO2 nanotube coating that can release antibacterial peptide in a controlled manner on the surface of titanium. Methods: TiO2 nanotubes were constructed on the surface of titanium by anodizing. The antibacterial peptide HHC-36 was combined with poly lactic-co-glycolic acid (PLGA) microspheres, and they were loaded on the TiO2 nanotubes by double solvent emulsification and volatilization technology. The morphology of the specimens was observed with SEM. Release curve of the antibacterial peptide was investigated. The antibacterial activity of the experimental material was tested by observing and measuring the inhibition zone. Results: TiO2 nanotubes with diameter of (100±20) nm were constructed on the surface of titanium. After PLGA-HHC-36 was loaded on the TiO2 nanotube, microspheres with small pores could be observed with SEM. The experimental specimens showed sustained release of HHC-36 that lasted for at least 15 days. An inhibition zone with a diameter of 14 mm was observed around the experimental specimen and the inhibition zone showed no visible change after 10 days. Conclusion: A TiO2 nanotube coating that can release antibacterial peptide in a controlled manner was successfully constructed on the surface of titanium. The experimental material revealed long-lasting antibacterial property.

Key words: TiO2 nanotube, PLGA microsphere, antimicrobial coating, antibiotic peptide HHC-36