氯喹对MT01促进大鼠骨髓间充质干细胞增殖作用的影响

doi:DOI

口腔医学研究 ›› 2015, Vol. 31 ›› Issue (11): 1061-1063.DOI: DOI

• 基金研究论著 • 下一篇

氯喹对MT01促进大鼠骨髓间充质干细胞增殖作用的影响

李维婷¹,李博²,丁子清²,崔野³,高涵²,刘引²,申玉芹^2*

1. 北京大学口腔医院第二门诊部牙周科北京 100000;
2. 吉林大学口腔医院牙周病科吉林长春 130021;
3. 吉林大学口腔医院正畸科吉林长春 130021

收稿日期:2015-05-29 出版日期:2015-11-28 发布日期:2016-03-21
通讯作者: 申玉芹,电话:0431-88796039
作者简介:李维婷(1977~),女,吉林省吉林市人,主治医师,硕士,主要从事牙周病感染病因学研究及牙周临床工作。
基金资助:
国家自然科学基金面上项目(编号:81371153)
吉林省科技厅自然科学基金(20150101173JC)

Effect of Chloroquine on the Proliferation of Rats Bone Marrow Mesenchymal Stem Cells Irritated by MT01.

LI Wei-ting¹, LI Bo², DING Zi-qing², CUI Ye³, GAO Han², LIU Yin², SHEN Yu-qin².

1. Department of Periodontology, The Second Outpatient, Stomatology Hospital, Peking University, Beijing 10000, China; 2. Department of Periodontology, Stomatology Hospital, Jilin University, Changchun 130021, China; 3. Department of Orthodontics, Stomatology Hospital, Jilin University, Changchun 130021, China

Received:2015-05-29 Online:2015-11-28 Published:2016-03-21

摘要/Abstract

摘要： 目的:通过检测氯喹(chloroquine,CQ) 对MT01促进Wistar大鼠骨髓间充质干细胞(BMSCs)增殖的影响,证实MT01可能经由内吞小体对BMSCs细胞生物学性能产生影响。方法:不同工作浓度的CQ与第3代Wistar大鼠BMSCs共培养24、48、72、96 h,检测CQ对BMSCs增殖影响的量效和时效关系;在此基础上,加入工作浓度为1 mg/L的MT01,MTT法检测不同浓度、不同时间点CQ对MT01作用下BMSCs增殖的影响。结果:适当浓度CQ(4mg/L)具有促进BMSCs增殖的作用;48 h CQ刺激BMSCs增殖最明显;低浓度CQ(1 mg/L、2 mg/L)对MT01促进BMSCs增殖具有抑制作用。结论:CQ对MT01促进BMSCs增殖具有抑制作用,提示MT01可能通过进入BMSCs内吞小体中发挥作用。

Abstract: Objective: To confirm that MT01 may influence the biological properties of bone marrow mesenchymal stem cells (BMSCs) via endosome by detecting the effect of chloroquine (CQ) on the promotion of Wistar rat BMSCs proliferation by MT01. Methods: The third generation of Wistar rat BMSCs were cultured with different working concentrations of CQ for 24,48,72 and 96 hours, in order to detect the effect of CQ on BMSCs proliferation in dose-response relationship and aging relationship. Then, MTT assay was employed to detect the influence of CQ on MT01-stimulated BMSCs proliferation at different concentrations and different time points by adding the 1mg/L working concentration of MT01. Results: Appropriate concentration of CQ (4mg//L) could promote BMSCs proliferation. CQ exhibited the strongest promotion to BMSCs proliferation after 48 hours. Low concentrations of CQ (1mg/L and 2mg/L) inhibited the MT01-mediated promotion of BMSCs proliferation. Conclusion: CQ has an inhibitory effect on the MT01-mediated promotion of BMSCs proliferation, suggesting that MT01 may play a role by entering the endosome in BMSCs.

中图分类号:

R780.1

李维婷,李博,丁子清,崔野,高涵,刘引,申玉芹. 氯喹对MT01促进大鼠骨髓间充质干细胞增殖作用的影响[J]. 口腔医学研究, 2015, 31(11): 1061-1063.

LI Wei-ting, LI Bo, DING Zi-qing, CUI Ye, GAO Han, LIU Yin, SHEN Yu-qin.. Effect of Chloroquine on the Proliferation of Rats Bone Marrow Mesenchymal Stem Cells Irritated by MT01.[J]. Journal of Oral Science Research, 2015, 31(11): 1061-1063.

参考文献

[1] Yang G, Wan M, Zhang Y, et al. Inhibition of a Crich oligodeoxynucleotide on activation of immune cells in vitro and enhancement of antibody response in mice [J]. Immunology, 2010, 131(4)∶501-512
[2] Yasuda K, Rutz M, Schlatter B, et al. CpG motifindependent activation of TLR9 upon endosomal translocation of "natural" phosphodiester DNA [J]. Eur J Immunol, 2006, 36(2)∶431-436
[3] Shen Y, Feng Z, Lin C, et al. An oligodeoxynucleotide that induces differentiation of bone marrow mesenchymal stem cells to osteoblasts in vitro and reduces alveolar bone loss in rats with periodontitis [J]. Int J Mol Sci, 2012, 13(3)∶2877-2892
[4] 申玉芹,孙新华,于丽,等.不同 CpG ODN对大鼠骨髓间充质干细胞增殖作用的研究[J].口腔医学研究,2009,25(6)∶723-725
[5] Nagar J, Ranade S, Kamath V, et al. Therapeutic potential of chloroquine in a murine model of inflammatory bowel disease [J]. Int Immunopharmacol, 2014, 21(2)∶328-335
[6] Bijker EM, Nganou-Makamdop K, van Gemert GJ, et al. Studying the effect of chloroquine on sporozoite-induced protection and immune responses in Plasmodium berghei malaria [J]. Malar J, 2015 Mar 26;14:130. doi: 10.1186/s12936-015-0626-2
[7] Joshi P, Chakraborti S, Ramirez-Vick JE, et al. The anticancer activity of chloroquine-gold nanoparticles against MCF-7 breast cancer cells [J]. Colloids Surf B Biointerfaces, 2012, 95∶195-200
[8] Tuomela J, Sandholm J, Kauppila JH, et al. Chloroquine has tumor inhibitory and tumor promoting effects in triple negative breast cancer [J]. Oncol Lett, 2013, 6(6)∶1665-1672
[9] Juge N, Moriyama S, Miyaji T, et al. Plasmodium falciparum chloroquine resistance transporter is a H+-coupled polyspecific nutrient and drug exporter [J]. Proc Natl Acad Sci U S A, 2015, 112(11)∶3356-3361
[10] Zhou D, Liu Y, Xu L H, et al. Chloroquine Differentially Modulates Inflammatory Cytokine Expression in RAW 264.7 Cells in Response to Inactivated Staphylococcus aureus [J]. Inflammation, 2015, 38(2)∶745-755
[11] Rungsihirunrat K, Muhamad P, Chaijaroenkul W, et al. Plasmodium vivax Drug Resistance Genes; Pvmdr1 and Pvcrt-o Polymorphisms in Relation to Chloroquine Sensitivity from a Malaria Endemic Area of Thailand [J]. Korean J Parasitol, 2015, 53(1)∶43-49
[12] van Kasteren SI, Overkleeft HS. Endo-lysosomal proteases in antigen presentation [J]. Curr Opin Chem Biol, 2014, 23∶8-15
[13] Chopra A, Saluja M, Venugopalan A. Effectiveness of chloroquine and inflammatory cytokine response in patients with early persistent musculoskeletal pain and arthritis following chikungunya virus infection [J]. Arthritis Rheumatol, 2014, 66(2)∶319-326
[14] Lee BL, Barton GM. Trafficking of endosomal Toll-like receptors [J]. Trends Cell Biol, 2014, 24(6)∶360-369
[15] Hazeki K, Nigorikawa K, Hazeki O. The Role of PIKfyve in Toll-Like Receptor-Mediated Responses[M]//Inflammation and Immunity in Cancer. Springer Japan, 2015∶49-62

氯喹对MT01促进大鼠骨髓间充质干细胞增殖作用的影响

Effect of Chloroquine on the Proliferation of Rats Bone Marrow Mesenchymal Stem Cells Irritated by MT01.

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