雌激素缺乏对大鼠根尖周炎病变区TLR4表达的影响

口腔医学研究 ›› 2015, Vol. 31 ›› Issue (8): 755-.

雌激素缺乏对大鼠根尖周炎病变区TLR4表达的影响

关晓月,钱华,边专^*

武汉大学口腔医学院·口腔生物医学教育部重点实验室湖北武汉 430079

收稿日期:2015-01-20 出版日期:2015-08-28 发布日期:2016-05-04
通讯作者: 边专,E-mail:bz@whuss.com
作者简介:关晓月(1988~ ),女,锡伯族,新疆人,硕士在读,主要研究牙体牙髓病学临床及基础研究工作。
基金资助:
国家自然科学基金重点项目(编号:30930099);国家自然科学基金国际(地区)合作与交流项目(编号:81120108010 )

Effect of Estrogen Deficiency on the Expression of TLR4 in Periapical Lesions of Rats

GUAN Xiao-yue, QIAN Hua, Bian Zhuan

School and Hospital of Stomatology, Key Lab for Oral Biomedical Engineering of Ministry of Education, Wuhan University, Wuhan 430079

Received:2015-01-20 Online:2015-08-28 Published:2016-05-04

摘要/Abstract

摘要： 目的:探究雌激素缺乏对大鼠根尖周炎病变区TLR4表达的影响。方法:30只大鼠随机分为两组:OVX组、SHAM组,暴露下颌第一磨牙髓腔,分别于开髓后 0、7、14、21 d处死大鼠。HE染色、酶组织化学染色、免疫组织化学法观测根尖周组织骨吸收范围,破骨细胞数及TLR4的表达。结果:与SHAM组相比,OVX组根尖周炎病变区骨吸收范围增大、破骨细胞数升高、TLR4的表达增强。结论:雌激素缺乏可增强大鼠根尖周炎病变区TLR4的表达。

关键词: 雌激素缺乏, 破骨细胞, TLR4, 根尖周炎

Abstract: Objective: To investigate the effects of estrogen deficiency on the expression of TLR4 in periapical lesions of rats. Methods: Thirty female rats were randomly divided into 2 groups: OVX group and SHAM group. The pulp cavities of mandibular first molars of rats were exposed. The rats were sacrificed at 0, 7, 14 and 21days after the exposure. Histologic examination for periapical bone loss area, enzyme histochemical examination for tartrate-resistant acid phosphatase (TRAP), and immunohistochemical examination for TLR4 were performed. Results: The alveolar bone loss area, osteoclasts number, and the expression of TLR4 in OVX group were higher than those of SHAM group. Conclusion: Estrogen deficiency increased the expression of TLR4 in the periapical lesions of rats.

Key words: Estrogen deficiency, Osteoclasts, TLR4, Periapical lesions

中图分类号:

R781.3

关晓月,钱华,边专. 雌激素缺乏对大鼠根尖周炎病变区TLR4表达的影响[J]. 口腔医学研究, 2015, 31(8): 755-.

GUAN Xiao-yue, QIAN Hua, Bian Zhuan. Effect of Estrogen Deficiency on the Expression of TLR4 in Periapical Lesions of Rats[J]. Journal of Oral Science Research, 2015, 31(8): 755-.

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