口腔医学研究

口腔医学研究 ›› 2016, Vol. 32 ›› Issue (8): 869-873.DOI: 10.13701/j.cnki.kqyxyj.2016.08.023

• 临床研究论著 • 上一篇    下一篇

钴离子对人肾小管上皮细胞毒性及Bax、Bcl-2基因表达变化的实验研究

罗新年,连文伟*   

  1. 南昌大学附属口腔医院修复科 江西 南昌 330006
  • 收稿日期:2015-12-23 出版日期:2016-08-26 发布日期:2016-08-26
  • 通讯作者: 连文伟,电话:0791-86232173
  • 作者简介:罗新年(1987~ ),男,江西人,硕士,医师,主要从事口腔修复与种植的临床治疗工作。

Effect of Co2+ on the Cytotoxicity of Human Renal Tubular Epithelial Cells and Gene Expression of Bax and Bcl-2

LUO Xin-nian, LIAN Wen-wei*   

  1. Department of Prosthodontics, Affiliated Stomatology Hospital of Nanchang University, Nanchang 330006, China
  • Received:2015-12-23 Online:2016-08-26 Published:2016-08-26

摘要： 目的:研究Co2+对人肾小管上皮细胞的毒性及对凋亡基因Bax、Bcl-2表达变化的影响。方法:体外培养肾小管上皮细胞,以5种浓度(0~10 μmol/L)的Co2+培养液培养细胞12 h、24 h、48 h。倒置显微镜下观察和记录细胞生长状态,采用MTT法检测各培养组在不同时间点的细胞增殖率,RT-PCR 法检测各培养组在不同时间点Bax、Bcl-2的表达变化,以评价Co2+的细胞毒性。结果:当Co2+作用浓度在7.5 μmol/L以下,观察时间内对细胞无毒性;在7.5 μmol/L以上时,刺激24 h以后对细胞有轻度毒性。随着Co2+作用浓度和时间的增加,各实验各组Bax mRNA表达量逐渐上调,Bcl-2 mRNA表达量逐渐下调,Bax/Bcl-2的比值升高。结论:Co2+抑制肾小管上皮细胞的增殖,低浓度Co2+对肾小管上皮细胞不存在毒性作用,高浓度时(≥7.5 μmol/L对细胞有轻度毒性,诱导细胞发生凋亡。

关键词: 钴离子, 人肾小管上皮细胞, 细胞毒性

Abstract: Objective: To study the effect of Co2+ on the cytotoxicity of human renal tubular epithelial cells and the gene expression of Bax and Bcl-2. Methods: HK-2 cells were cultured and incubated with five different concentrations of Co2+ solution(0-10 μmol/L)for 12h, 24h and 48h in vitro. Cells were observed and recorded under inverted phase contrast microscope. Cell proliferation of each culture group was detected by MTT and the expression of Bax and Bcl-2 were detected by RT-PCR at different time points. Results: Co2+ had no toxicity on HK-2 cells when the concentration was lower than 7.5 μmol/L during the observation time. While when the concentration was higher than 7.5 μmol/L, Co2+ had mild toxicity on HK-2 cells after 24h. As to the increase of Co2+ concentration and time of duration, the mRNA expression of Bax in each experimental group gradually increased, while the mRNA expression of Bcl-2 gradually decreased and the value of Bax/Bcl-2 increased. Conclusion: Co2+ inhibited the proliferation of HK-2 cells. It has no toxicity on human renal tubular epithelial cells at low concentration,but has mild toxicity and promotes cell apoptosis at high concentration(≥7.5 μmol/L).

Key words: Cobaltion, Human renal tubular epithelial cells, Cytotoxicity

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