口腔医学研究

口腔医学研究 ›› 2018, Vol. 34 ›› Issue (4): 388-392.DOI: 10.13701/j.cnki.kqyxyj.2018.04.013

• 牙髓病学研究 • 上一篇    下一篇

5种根管冲洗液对小鼠成纤维细胞L929的毒性评价

史彦1*, 邓志鹏1, 陈浩2   

  1. 1. 江西省口腔生物重点实验室,南昌大学附属口腔医院牙体牙髓科 江西 南昌 330006;
    2. 江西省中医药大学附属医院药剂科 江西 南昌 330006
  • 收稿日期:2017-12-03 出版日期:2018-04-28 发布日期:2018-04-25
  • 通讯作者: 史彦,E-mail:shiyan1018@outlook.com
  • 作者简介:史彦(1976~ ),女,江西南昌人,博士在读,副主任医师,主要从事牙体牙髓病学研究。
  • 基金资助:
    江西省科技厅基金项目(编号:2015BBG70239)

Cytotoxicity of Five Kinds of Irrigants on Mouse Fibroblasts L929.

SHI Yan1*, DENG Zhi-peng1, CHEN Hao2.   

  1. 1. Jiangxi Provincial Key Laboratory of Oral Biology ,Department of Conservative Dentistry and Endodontics, Stomatology Hospital of Nanchang University, Nanchang 330006, China;
    2. Department of Pharmacy, The Affiliated Hospital of Traditional Chinese Medicine of Jiangxi University, Nanchang 330004, China.
  • Received:2017-12-03 Online:2018-04-28 Published:2018-04-25

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摘要： 目的:评价复方冰硼冲洗液、冰硼冲洗液、次氯酸钠溶液、金栀洁龈含漱液和复方氯己定含漱液的细胞毒性。方法:体外培养L929细胞,选用复方冰硼冲洗液(A组),冰硼冲洗液(B组),NaClO(C组),金栀洁龈含漱液(D组),复方氯己定含漱液(E组),培养基作为空白对照组,分别与L929接触6、12、24、48 h,用MTT法测细胞增殖率。结果:高浓度冲洗液一般比低浓度具有更强的细胞生长抑制作用。A组和B组对L929无明显细胞毒性, D组有中度细胞毒性,C组和E组均有较强细胞毒性。冲洗液细胞毒性由高到低排序为:E组,C组,D组,A组,B组。结论:中药制剂复方冰硼冲洗液对L929细胞无明显细胞毒性,认为可临床用于根管冲洗。

关键词: 复方冰硼冲洗液, 根管冲洗, 细胞毒性, L929细胞

Abstract: Objective: To evaluate the cytotoxicity of Fufang Bingpeng Irrigants, Bingpeng Irrigants,sodium hypochloride,jinzhi gargle, and chlorhexidine gluconatie compound gargle on mouse fibroblasts L929. Methods: L929 fibroblasts were cultured in vitro. Cells were cultured with five irrigants for 6 hours, 12 hours, 24 hours, and 48 hours. The culture medium was set as the control group. The MTT assay was employed to evaluate the cytotoxicity. Results: Higher concentrations of irrigants generally showed significantly stronger inhibitory effects. Fufang Bingpeng Irrigants and Bingpeng Irrigants showed no cytotoxicity,jinzhi gargle showed middle, while sodium hypochloride and chlorhexidine displayed higher toxicity. The rank of the irrigation cytotoxicity from the most to the least was: chlorhexidine, sodium hypochloride, jinzhi gargle, Bingpeng Irrigants, and Fufang Bingpeng Irrigants. Conclusion: The Fufang Bingpeng Irrigants showed no detectable cytotoxicity. As a root canal irrigant, Chinese medicine alteratives might be adopted.

Key words: Fufang Bingpeng irrigants, Root canal irrigant, Cytotoxicity, L929 fibroblasts