MMP-9基因敲除小鼠磨牙牙胚中釉原蛋白和成釉蛋白表达的研究

doi:10.13701/j.cnki.kqyxyj.2016.11.002

口腔医学研究 ›› 2016, Vol. 32 ›› Issue (11): 1117-1121.DOI: 10.13701/j.cnki.kqyxyj.2016.11.002

MMP-9基因敲除小鼠磨牙牙胚中釉原蛋白和成釉蛋白表达的研究

李桐,袁国华,聂敏,杨国斌^*

武汉大学口腔医学院口腔生物医学工程教育部重点实验室湖北武汉 430079

收稿日期:2016-06-27 出版日期:2016-11-25 发布日期:2016-11-25
通讯作者: 杨国斌,E-mail: Guobin.yang@whu.edu.cn
作者简介:李桐(1991~ ),女,山西人,硕士在读,主要从事牙体牙髓病学研究工作。
基金资助:
国家自然科学基金资助项目(编号:81371105,81570942)

Expression of Amelogenin and Ameloblastin in the Molar Tooth Germs of MMP-9 Knock-out Mice

LI Tong, YUAN Guo-hua, NIE Min, YANG Guo-bin^*

School and Hospital of Stomatology, Key Lab for Oral Biomedical Engineering of Ministry of Education, Wuhan University, Wuhan 430079, China.

Received:2016-06-27 Online:2016-11-25 Published:2016-11-25

摘要/Abstract

摘要： 目的:检测MMP-9基因敲除小鼠(MMP-9^-/-)和野生型小鼠磨牙牙胚中釉原蛋白和成釉蛋白的表达。方法:使用免疫组化方法检测出生后2 d(P2)、5 d(P5)、7 d(P7)野生型小鼠和MMP-9^-/-小鼠下颌磨牙牙胚中釉原蛋白和成釉蛋白的表达分布。结果:在P2时,釉原蛋白主要表达在成熟的成釉细胞胞、及其相邻的成牙本质细胞内,敲除MMP-9后,釉原蛋白的表达可扩散至牙乳头间充质;在P5和P7时,釉原蛋白的表达分布在野生型小鼠和MMP-9^-/-小鼠中无差异,但在MMP-9^-/-小鼠中釉原蛋白在成釉细胞中的表达量明显增强。在P2、P5和P7时,成釉蛋白的表达分布和表达量在野生型小鼠和MMP-9^-/-小鼠中均无差异。结论:MMP-9可能通过酶切釉原蛋白而影响其表达分布。

关键词: 釉原蛋白, 成釉蛋白, 磨牙牙胚, 基质金属蛋白酶9

Abstract: Objective: To detect the expressions of Amelogenin and Ameloblastin in wild-type mice and MMP-9 gene knock-out mice (MMP-9^-/-). Methods: The expression levels of Amelogenin and Ameloblastin in tooth germs were detected with immunohistochemistry in wild-type and MMP-9^-/- mice at post-natal day 2 (P2), 5 (P5), and 7 (P7). Results: At P2, Amelogenin was mainly expressed in matured ameloblasts and the underlying odontoblasts. However, in MMP-9^-/- mice, the expression of amelogenin was expanded to the dental mesenchyme. No difference was found in the expression pattern of Amelogenin between wild-type mice and MMP-9^-/- mice at P5 and P7. However, the expression level of Amelogenin in ameloblasts was highly increased in MMP-9^-/- mice compared with that in wild-type mice. No obvious differences were detected in the expression pattern or expression level of Ameloblastin between wild-type mice and MMP-9^-/- mice at P2, P5, and P7. Conclusion: MMP-9 might be able to degrade Amelogenin, and further influence the expression pattern of Amelogenin.

Key words: Amelogenin, Ameloblastin, Molar germ, Matrix, Metalloproteinase-9

中图分类号:

Q132

李桐,袁国华,聂敏,杨国斌. MMP-9基因敲除小鼠磨牙牙胚中釉原蛋白和成釉蛋白表达的研究[J]. 口腔医学研究, 2016, 32(11): 1117-1121.

LI Tong, YUAN Guo-hua, NIE Min, YANG Guo-bin. Expression of Amelogenin and Ameloblastin in the Molar Tooth Germs of MMP-9 Knock-out Mice[J]. Journal of Oral Science Research, 2016, 32(11): 1117-1121.

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MMP-9基因敲除小鼠磨牙牙胚中釉原蛋白和成釉蛋白表达的研究

Expression of Amelogenin and Ameloblastin in the Molar Tooth Germs of MMP-9 Knock-out Mice

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