口腔医学研究

口腔医学研究 ›› 2016, Vol. 32 ›› Issue (9): 893-896.DOI: 10.13701/j.cnki.kqyxyj.2016.09.001

• 基础研究论著 •    下一篇

不同强度静磁场促进成骨细胞增殖和分化的研究

禹怡君1,孙卫斌2,任双双2,刘超3,苗雷英1*   

  1. 1. 南京大学医学院附属口腔医院牙体牙髓科 江苏 南京 210008;
    2. 南京大学医学院附属口腔医院牙周病科 江苏 南京 210008;
    3. 南京大学医学院附属口腔医院正畸科 江苏 南京 210008
  • 收稿日期:2016-01-25 出版日期:2016-09-26 发布日期:2016-09-26
  • 通讯作者: 苗雷英,E-mail:miaoleiying80@163.com
  • 作者简介:禹怡君(1992~ ),女,江苏省盐城市人,硕士在读,主要从事磁性材料促进骨再生方面的研究工作。
  • 基金资助:
    国家自然科学基金项目(编号:51472115,81300852,81570952)
    江苏省自然科学基金青年基金(编号:BK20130079)
    南京市六大人才高峰项目(编号:WSW-049)
    南京市医学科技发展项目(编号: ykk14109)

Effects of Static Magnetic Field at Different Intensity on Proliferation and Differentiation of Osteoblast Cells.

YU Yi-jun1, SUN Wei-bin2, REN Shuang-shuang2, LIU Chao3, MIAO Lei-ying1*   

  1. 1. Department of Endodontics, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing 210008, China;
    2. Department of Periodontology, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing 210008, China;
    3. Department of Orthodontics, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing 210008, China
  • Received:2016-01-25 Online:2016-09-26 Published:2016-09-26

PDF (PC)

331

被引次数

2

摘要： 目的:研究不同强度磁场对成骨细胞增殖与分化的影响,为寻求合适的静磁场作用强度提供实验基础。方法:体外培养Wistar大鼠颅骨成骨细胞,分别置于0Gs,300Gs、1400Gs、2000Gs不同磁场强度的静磁场中作用。在1、3、5、7 d用CCK-8法检测成骨细胞的增殖情况,流式细胞仪测定不同磁场强度下48h时细胞所处的周期,q-PCR检测磁场作用下7 d成骨细胞内成骨因子OPN,Runx2表达情况。结果:结果显示各组均出现不同程度的促增殖作用,尤以300Gs组作用7 d时显著。300Gs磁场作用组S期和G2/M期细胞百分比显著增加,推测一定磁场强度可促使静止态的G0/G1期细胞活跃向S 期转变,细胞增殖加速。在300Gs磁场作用7 d后,成骨因子OPN,Runx2表达都显著增高。结论:实验采用的300Gs强度磁场可有效促进成骨细胞的增殖与分化。

关键词: 成骨细胞, 静磁场, 细胞增殖, 成骨分化

Abstract: Objective: To observe the effects of different intensity static magnetic fields (SMFs) on the proliferation and differentiation of rat osteoblast cells. Methods: Rat calvarias osteoblasts were cultured and incubated in the SMFs of 0Gs, 300Gs, 1400Gs and 2000Gs. CCK-8 method was used to detect osteoblast proliferation at day 1, 3, 5 and 7. Flow cytometry was employed to detect the cell cycle of osteoblasts under different intensity magnetic fields. Expressions of osteogenic marker genes like OPN and Runx2 were measured by quantitative polymerase chain reaction. Results: The proliferation of osteoblasts exposed to 300Gs SMF for 7 days increased obviously with significant difference as compared with 0Gs group (P<0.05). Moreover, after magnetic exposure with intensity of 300Gs, G0/G1 phase percentage decreased, with S phase and G2 phase percentage significantly increased as compared with the control. The expression of OPN and Runx2 in osteoblast exposure to 300Gs and 2000Gs were significantly higher than the control group, which suggested that magnetic field of intensity of 300Gs had the ability to induce osteogenic differentiation of osteoblasts. Conclusion: Static magnetic field of intensity of 300Gs could increase the proliferation and differentiation of osteoblasts.

Key words: Osteoblast Static magnetic field, Cell proliferation, Osteogenic differentiation

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