静磁场对大鼠下颌骨骨折愈合的研究

doi:10.13701/j.cnki.kqyxyj.2020.06.011

口腔医学研究 ›› 2020, Vol. 36 ›› Issue (6): 548-553.DOI: 10.13701/j.cnki.kqyxyj.2020.06.011

静磁场对大鼠下颌骨骨折愈合的研究

刘阳¹, 李善昌^1*, 王长振^2*, 周红梅², 董国福², 赵雪龙², 刘琦²

1.佳木斯大学附属口腔医院黑龙江佳木斯 154002;
2.军事科学院军事医学研究院辐射医学研究所北京 100850

收稿日期:2019-10-31 出版日期:2020-07-03 发布日期:2020-07-06
通讯作者: ^*李善昌,E-mail:lsc6930@163.com;王长振,E-mail:wangcz2002@aliyun.com
作者简介:刘阳(1990~ ),男,山东枣庄人,硕士,主要从事口腔颌面外科方向研究。
基金资助:
国家自然科学基金(编号:31800053);北京市自然科学基金(编号:5194029、4194097)

Effect of Static Magnetic Field on Mandibular Fracture Healing in Rats

LIU Yang¹, LI Shanchang^1*, WANG Changzhen^2*, ZHOU Hongmei², DONG Guofu², ZHAO Xuelong², LIU Qi

1. Department of Affiliated Stomatological Hospital, Jiamusi University, Jiamusi 154002, China;
2. Department of Institute of Radiation Medicine, Military Academy of Military Sciences, Beijing 100850, China

Received:2019-10-31 Online:2020-07-03 Published:2020-07-06

摘要/Abstract

摘要： 目的: 研究静磁场(Static Magnetic Field,SMF)对大鼠下颌骨骨折愈合过程的影响。方法: SD大鼠60只,随机分为3组:骨折手术(C组);骨折手术+骨折处覆盖陶瓷(S组);骨折手术+骨折处覆盖磁场强度为0.1T的钕磁铁(L组)。术后每周考察SD大鼠的体重、饮食、饮水;在2、4、6、8周取材。X线片检查骨折区愈合情况;ELISA检测血清中BMP-2(bone morphogenetic protein-2,BMP-2)、IL-1(Interleukin-1,IL-1)表达情况;偶氮胂Ⅲ法检测血清钙值;HE(hematoxylin-eosin staining)观察骨折区;免疫组化检测成骨相关基因BMP-2、OPG(osteoprotegerin,OPG)、OCN(osteocalcin,OCN)。结果: X线结果显示L组的骨折愈合效果最好;L组血清中的BMP-2、IL-1、钙值表达高于S、C组;HE染色观察L组新生骨小梁数量明显多于C组和S组;免疫组化结果显示静磁场可以促进BMP-2、OPG、OCN的合成和分泌。结论: 静磁场可有效促进骨折的愈合。

关键词: 静磁场, 骨折愈合, 成骨因子

Abstract: Objective: To study the effect of static magnetic field on the healing process of mandibular fracture in rats. Methods: 60 SD rats were randomly divided into three groups: fracture operation (group C), fracture operation + fracture covered ceramic (group S), fracture surgery + neodymium magnet with 0.1T covering magnetic field at fracture site (Group L). The body weight, diet, and drinking water of SD rats were investigated every week after operation, and the samples were taken after 2, 4, 6, and 8 weeks. Bone fracture healing was observed by X-ray examination. Expression of BMP-2 and IL-1 in serum was detected by ELISA. Serum calcium was determined by arsenazo Ⅲ method. Fracture area was observed by HE staining. Osteogenesis related genes BMP-2, OPG, and OCN were immunohistochemical detected. Results: X-ray results showed that group L had the best effect of fracture healing, the expressions of BMP-2, IL-1, and calcium in group L were higher than those in group S and C. HE staining showed that the number of new bone trabeculae in group L was significantly more than that in group C and group S. Immunohistochemical results showed that magnetostatic field could promote the synthesis and secretion of BMP-2, OPG, and OCN. Conclusion: Static magnetic field can effectively promote fracture healing.

Key words: static magnetic field, fracture healing, osteogenic factor

刘阳, 李善昌, 王长振, 周红梅, 董国福, 赵雪龙, 刘琦. 静磁场对大鼠下颌骨骨折愈合的研究[J]. 口腔医学研究, 2020, 36(6): 548-553.

LIU Yang, LI Shanchang, WANG Changzhen, ZHOU Hongmei, DONG Guofu, ZHAO Xuelong, LIU Qi. Effect of Static Magnetic Field on Mandibular Fracture Healing in Rats[J]. Journal of Oral Science Research, 2020, 36(6): 548-553.

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静磁场对大鼠下颌骨骨折愈合的研究

Effect of Static Magnetic Field on Mandibular Fracture Healing in Rats

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